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基质金属蛋白酶-9基因多态性与儿童幽门螺杆菌相关性胃十二指肠疾病的关系
单庆文, 经承学, 王琳琳, 吕自力, 唐清, 云翔, 连淑君
中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (04) : 262-266.
PDF(1127 KB)
PDF(1127 KB)
基质金属蛋白酶-9基因多态性与儿童幽门螺杆菌相关性胃十二指肠疾病的关系
Relationship between gene polymorphisms in MMP-9 and Helicobacter pylori-related upper gastrointestinal disease in children
目的：研究基质金属蛋白酶-9（MMP-9）基因启动子多态性与儿童幽门螺杆菌（Hp）相关性慢性胃炎、十二指肠溃疡的易感性和临床特征的关系。方法：采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)和测序方法，对100例慢性胃炎及32例十二指肠溃疡儿童和102例健康对照组儿童的MMP-9 基因-1562C/T多态性进行检测。采用反转录聚合酶链反应(RT-PCR)方法检测胃黏膜MMP-9 mRNA的表达。结果：MMP-9基因-1562 C/T多态性位点的基因型和等位基因频率在病例组和对照组儿童中的分布无明显差别。在慢性胃炎组中，C/C基因型携带者发生Hp感染的风险是T等位基因（C/T+T/T）携带者的3.1倍。MMP-9 基因-1562C/T多态性不影响MMP-9 mRNA在胃黏膜中的表达水平。结论：MMP-9 基因-1562C/T多态性与儿童慢性胃炎和十二指肠溃疡的发生无相关性，该基因位点的C/C基因型携带者可能与Hp易感性相关。［中国当代儿科杂志，2010，12（4）：262-266］
OBJECTIVE: To investigate the relationship of the promoter of matrix metalloproteinase-9 (MMP-9) gene polymorphisms with the susceptibility and clinical features of Helicobacter pylori (H. pylori)-related chronic gastritis and duodenal ulcer in children. METHODS: One hundred children with chronic gastritis, 32 children with duodenal ulcer and 102 healthy children were enrolled．The promoter of MMP-9-1562C/T gene polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and sequencing. MMP-9 mRNA expression in gastric mucosa was confirmed by reverse transcription polymerase chain reaction. RESULTS: The genotype distributions and allele frequencies of MMP-9-1562C/T gene polymorphisms were similar in gastric upper gastrointestinal disease and healthy subjects. The relative risk for H.pylori infection in C/C genetype carriers was 3.1 times as high as that in T allele (C/T+T/T) carriers in children with chronic gastritis. MMP-9-1562 C/T gene polymorphisms did not affect MMP-9 mRNA expression level. CONCLUSIONS: These data suggest that MMP-9-1562 C/T gene polymorphisms are not associated with susceptibility to chronic gastritis and duodenal ulcer in children. The C/C genotype of MMP-9-1562 C/T gene polymorphism might be associated with H.pylori infection.［Chin J Contemp Pediatr, 2010, 12 (4):262-266］
基质金属蛋白酶-9 / 基因多态性 / 幽门螺杆菌 / 胃十二指肠疾病 / 儿童
Matrix metalloproteinase-9 / Gene polymorphism / Helicobacter pylori / Upper gastrointestin disease / Child
［1］Kuipers EJ, Thijs JC, Festen HP. The prevalence of Helicobacter pylori in peptic ulcer disease［J］. Aliment Pharmacol Ther, 1995, 9(Suppl 2):59-69.
［2］卢燕鸣,曹兰芳,孔宪明.小儿幽门螺杆菌相关性疾病［J］.中国当代儿科杂志, 2002, 4(6):567-568.
［3］中华医学会儿科学分会感染消化学组.小儿慢性胃炎、消化性溃疡胃镜诊断标准［J］.中华儿科杂志,2003,41(3):189.
［4］Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis: The updated Sydney System［J］. Am J Surg Pathol, 1996, 20(10):1161-1181.
［5］中华医学会消化病学分会.幽门螺杆菌共识意见(2003·安徽桐城) ［J］.现代消化及介人诊疗, 2004,9(1):59-60.
［6］Kubben FJ, Sier CF, Meijer MJ, van den Berg M, van der Reijden JJ, Griffioen G, et al. Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer［J］.Br J Cancer, 2006,95(6):744-751.
［7］Shan K, Lian-Fu Z, Hui D, Wei G, Na W, Xia J, et al. Polymorphisms in the promoter regions of the matrix metalloproteinases-7,-9 and the risk of endometriosis and adenomyosis in China［J］. Mol Human Reprod,2006,12(1): 35-39.
［8］Matsumura S,Oue N,Nakayama H,Kitadai Y,Yoshida K,Yamaguchi Y, et al. A single nucleotide polymorphism in the MMP-9 promoter affects tumor progression and invasive phenotype of gastric cancer［J］. J Cancer Res Clin Oncol,2005,131(1): 19-25.
［9］Granstrom M, Tindberg Y, Blennow M. Seroepidemiology of Helicobacter pylori infection in a cohort of children monitored from 6 monthsto 11 years of age［J］. J Clin Microbiol,1997, 35(2):468-470.
［10］Thomas JE, Dale A, Harding M, Coward WA, Cole TJ, Weaver LT. Helicobacter pylori colonization in early life［J］. Pediatr Res, 1999, 45(2):218-223.
［11］Berginl PJ, Raghavan1 S,Svensson H,Starckx S,Van-Aelst I,Gjertsson I,et al.Gastric gelatinase B/matrixmetalloproteinase-9 is rapidly increased in Helicobacter felis-induced gastritis［J］. FEMS Immunol Med Microbiol, 2008, 52(1):88-98.
［12］Zhang B,Ye S, Herrmann SM, Eriksson P, de Maat M, Evans A, et al. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis［J］. Circulation,1999,99(14):1788-1794.
［13］Kubben FJ, Sier CF, Schram MT,Witte AM,Veenendaal RA,van Duijn W,et al.Eradication of Helicobacter pylori infection favourably affects altered gastric mucosal MMP-9 levels［J］. Helicobacter,2007,12(5):498-504.
［14］Mori N, Sato H, Hayashibara T, Senba M, Geleziunas R, Wada A, et al. Helicobacter pylori induces matrix metalloproteinase-9 through activation of nuclear factor κB ［J］.Gastroenterology, 2003, 124 (4):983-992.
［15］Bergin PJ, Anders E, Sicheng W,Erik J,Jennie A,Hans L,et al. Increased production of matrix metalloproteinases in Helicobacter pyloriassociated human gastritis［J］.Helicobacter,2004, 9(3): 201-210.
