Abstract：OBJECTIVE: To investigate the relationship of the promoter of matrix metalloproteinase-9 (MMP-9) gene polymorphisms with the susceptibility and clinical features of Helicobacter pylori (H. pylori)-related chronic gastritis and duodenal ulcer in children. METHODS: One hundred children with chronic gastritis, 32 children with duodenal ulcer and 102 healthy children were enrolled．The promoter of MMP-9-1562C/T gene polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and sequencing. MMP-9 mRNA expression in gastric mucosa was confirmed by reverse transcription polymerase chain reaction. RESULTS: The genotype distributions and allele frequencies of MMP-9-1562C/T gene polymorphisms were similar in gastric upper gastrointestinal disease and healthy subjects. The relative risk for H.pylori infection in C/C genetype carriers was 3.1 times as high as that in T allele (C/T+T/T) carriers in children with chronic gastritis. MMP-9-1562 C/T gene polymorphisms did not affect MMP-9 mRNA expression level. CONCLUSIONS: These data suggest that MMP-9-1562 C/T gene polymorphisms are not associated with susceptibility to chronic gastritis and duodenal ulcer in children. The C/C genotype of MMP-9-1562 C/T gene polymorphism might be associated with H.pylori infection.［Chin J Contemp Pediatr, 2010, 12 (4):262-266］
SHAN Qing-Wen,JING Cheng-Xue,WANG Lin-Lin et al. Relationship between gene polymorphisms in MMP-9 and Helicobacter pylori-related upper gastrointestinal disease in children[J]. CJCP, 2010, 12(04): 262-266.
［4］Dixon MF, Genta RM, Yardley JH, Correa P. Classification and grading of gastritis: The updated Sydney System［J］. Am J Surg Pathol, 1996, 20(10):1161-1181.
［6］Kubben FJ, Sier CF, Meijer MJ, van den Berg M, van der Reijden JJ, Griffioen G, et al. Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer［J］.Br J Cancer, 2006,95(6):744-751.
［7］Shan K, Lian-Fu Z, Hui D, Wei G, Na W, Xia J, et al. Polymorphisms in the promoter regions of the matrix metalloproteinases-7,-9 and the risk of endometriosis and adenomyosis in China［J］. Mol Human Reprod,2006,12(1): 35-39.
［8］Matsumura S,Oue N,Nakayama H,Kitadai Y,Yoshida K,Yamaguchi Y, et al. A single nucleotide polymorphism in the MMP-9 promoter affects tumor progression and invasive phenotype of gastric cancer［J］. J Cancer Res Clin Oncol,2005,131(1): 19-25.
［9］Granstrom M, Tindberg Y, Blennow M. Seroepidemiology of Helicobacter pylori infection in a cohort of children monitored from 6 monthsto 11 years of age［J］. J Clin Microbiol,1997, 35(2):468-470.
［10］Thomas JE, Dale A, Harding M, Coward WA, Cole TJ, Weaver LT. Helicobacter pylori colonization in early life［J］. Pediatr Res, 1999, 45(2):218-223.
［11］Berginl PJ, Raghavan1 S,Svensson H,Starckx S,Van-Aelst I,Gjertsson I,et al.Gastric gelatinase B/matrixmetalloproteinase-9 is rapidly increased in Helicobacter felis-induced gastritis［J］. FEMS Immunol Med Microbiol, 2008, 52(1):88-98.
［12］Zhang B,Ye S, Herrmann SM, Eriksson P, de Maat M, Evans A, et al. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis［J］. Circulation,1999,99(14):1788-1794.
［14］Mori N, Sato H, Hayashibara T, Senba M, Geleziunas R, Wada A, et al. Helicobacter pylori induces matrix metalloproteinase-9 through activation of nuclear factor κB ［J］.Gastroenterology, 2003, 124 (4):983-992.
［15］Bergin PJ, Anders E, Sicheng W,Erik J,Jennie A,Hans L,et al. Increased production of matrix metalloproteinases in Helicobacter pyloriassociated human gastritis［J］.Helicobacter,2004, 9(3): 201-210.
