支气管肺发育不良新生儿支气管肺泡灌洗液IL-8 SP-A和TGF-β1的表达

刘冬云; 吴静; 张小英; 封志纯

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中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (06) : 444-446.

论著·临床研究

支气管肺发育不良新生儿支气管肺泡灌洗液IL-8 SP-A和TGF-β1的表达

刘冬云，吴静，张小英，封志纯

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Expression of IL-8, SP-A and TGF-β1 in bronchoalveolar lavage fluid of neonates with bronchopulmonary dysplasia

LIU Dong-Yun, WU Jing, ZHANG Xiao-Ying, FENG Zhi-Chun

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摘要

目的：了解支气管肺发育不良（BPD）新生儿肺灌洗液IL-8、SP-A和TGF-β1的表达。方法：将我院2007年12月至2009年10月诊断为BPD的30名新生儿作为实验组。另外选同期30例非BPD患儿作为对照组，两组胎龄、性别、出生体重无差异。采用非纤维支气管镜支气管肺泡灌洗法进行肺灌洗，用ELISA法测定IL-8、SP-A和TGF-β1的含量。结果：BPD组肺泡灌洗液中TGF-β1、IL-8含量分别为47±15和54±16 μg/mL明显高于对照组的34±13和28±13 μg/mL（P<0.01）。BPD组SP-A含量明显低于对照组(35±16 μg/mL vs 42±14 μg/mL; P<0.05)。结论：TGF-β1和IL-8在BPD患儿肺灌洗液中有过高表达，可能与BPD患儿肺的异常发育与成熟有关。BPD组SP-A含量较低，提示给予SP-A外源性治疗有可能为BPD的治疗手段之一。［中国当代儿科杂志，2010，12（6）：444-446］

Abstract

OBJECTIVE: To investigate the expression of interleukin 8 (IL-8), surfactant protein-A (SP-A) and transforming growth factor β1 (TGF-β1) in bronchoalveolar lavage fluid (BALF) of neonates with bronchopulmonary dysplasia (BPD). METHODS: Thirty neonates with BPD and 30 gestational age-, gender-, and birth weight-matched neonates without BPD (control group) were enrolled from December 2007 to October 2009. Non-brochoscopic bronchoalveolar lavage was performed. The levels of IL-8, SP-A and TGF-β1 in BALF were measured using ELISA. RESULTS: The levels of TGFβ1 (47±15 μg/mL vs 34±13 μg/mL) and IL-8 (54±16 μg/mL vs 28±13 μg/mL) in the BPD group were significantly higher than those in the control group (P<0.01). In contrast, the contents of SP-A in the BPD group were significantly lower than those in the control group (35±16 μg/mL vs 42±14 μg/mL;P<0.05). CONCLUSIONS: The increased expression of TGF-β1 and IL-8 in BALF may be involved in abnormal lung development and maturation in neonates with BPD. The low expression of SP-A in the BPD group suggests that the exogenous SP-A administration may be an option for the treatment of BPD.［Chin J Contemp Pediatr, 2010, 12 (6):444-446］

导出引用

刘冬云, 吴静, 张小英, 封志纯. 支气管肺发育不良新生儿支气管肺泡灌洗液IL-8 SP-A和TGF-β1的表达[J]. 中国当代儿科杂志. 2010, 12(06): 444-446

LIU Dong-Yun, WU Jing, ZHANG Xiao-Ying, FENG Zhi-Chun. Expression of IL-8, SP-A and TGF-β1 in bronchoalveolar lavage fluid of neonates with bronchopulmonary dysplasia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(06): 444-446

中图分类号： R722

参考文献

［1］Taeusch HW, Ballard RA, Gleason CA. Avery's diseases of the newborn［M］.8th ed.Philadelphia:Elsevier Saunders, 2004:723-736.
［2］Jobe AH, Bancalari E. Bronchopulmonary dysplasia［J］.Am J Respir Crit Care Med, 2001, 163（7）:1723-1729.
［3］Been JV, Zimmermann LJ, Debeer A, Kloosterboer N, van Iwaarden JF.Bronchoalveolar lavage fluid from preterm infants with chorioamnionitis inhibits alveolar epithelial repair［J］.Respir Res, 2009, 23(10):116.
［4］Jonsson B, Li YH, Noack G, Brauner A, Tullus K. Downregulatory cytokines in tracheobronchial aspirate fluid from infants with chronic lung disease of prematurity ［J］. Acta Paediatr, 2000, 89(11):1375-1380.
［5］Gaede KI, Amicosante M, Schurmann M, Fireman E, Saltini C, Müller-Quernheim J. Function associated transforming growth factor-beta gene polymorphism in chronic beryllium disease［J］.J Mol Med, 2005, 83(5)： 397-405.
［6］Kunzmann S, Speer CP, Jobe AH, Kramer BW. Antenatal inflammation induced TGF-beta1 but suppressed CTGF in preterm lungs［J］.Am J Physiol Lung Cell Mol Physiol, 2007, 292(1)：223-231.
［7］Ichiba H, Saito M, Yamano T. Amniotic fluid transforming growth factor-beta(1) and the risk for the development of neonatal bronchopulmonary dysplasia［J］.Neonatology, 2009, 96(3):156-161.
［8］Harris WT, Muhlebach MS, Oster RA, Knowles MR, Noah TL .Transforming growth factor-beta(1) in bronchoalveolar lavage fluid from children with cystic fibrosis［J］.Pediatr Pulmonol, 2009, 44(11):1057-1064.
［9］Vicencio AG, Lee CG, Cho SJ, Eickelberg O, Chuu Y, Haddad GG, et al . Conditional overexpression of bioactive transforming growth factor-beta1 in neonatal mouse lung: a new model for bronchopulmonary dysplasia?［J］. Am J Respir Cell Mol Biol, 2004, 31(6)：650-656.
［10］Saunders FD, Westphal M, Enkhbaatar P, Wang J, Pazdrak K, Nakano Y, et al .Molecular biological effects of selective neuronal nitric oxide synthase inhibition in ovine lung injury［J］.Am J Physiol Lung Cell Mol Physiol, 2010, 298(3): 427-436.
［11］Brander L, Sinderby C, Lecomte F, Leong-Poi H, Bell D, Beck J, et al. Neurally adjusted ventilatory assist decreases ventilator-induced lung injury and non-pulmonary organ dysfunction in rabbits with acute lung injury［J］.Intensive Care Med, 2009, 35(11):1979-1989.
［12］Su BH, Chiu HY, Lin TW, Lin HC. Interleukin-8 in bronchoalveolar lavage fluid of premature infants at risk of chronic lung disease［J］.J Formos Med Assoc, 2005,104(4):244-248.
［13］Haczku A. Protective role of the lung collectins surfactant protein A and surfactant protein D in airway inflammation［J］.J Allergy Clin Immunol, 2008, 122(5):861-879.
［14］Todd DA, Marsh MJ, George A, Henderson NG, Barr H, Sebastian S, et al.Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children［J］.Pediatr Crit Care Med, 2010, 11(1):82-91.
［15］Yu ZW, Zhang JH. Effect of inhaled budesonide on surfactant protein expression in asthmatic mice［J］.Allergy Asthma Proc, 2008, 29(5):486-492.