维生素A调节小鼠肠黏膜淋巴细胞免疫功能的作用

杨凌云; 储波; 蒋丽蓉

PDF(930 KB)

中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (12) : 976-978.

论著·实验研究

维生素A调节小鼠肠黏膜淋巴细胞免疫功能的作用

杨凌云，储波，蒋丽蓉

作者信息 +

Effects of vitamin A on the immune function of intestinal mucosa lymphocytes in mice

YANG Ling-Yun, CHU Bo, JIANG Li-Rong

Author information +

文章历史 +

摘要

目的：探讨维生素A对小鼠外周血及肠黏膜T淋巴细胞亚群发育及肠黏膜分泌细胞因子水平的影响。方法：幼鼠共20只，随机分为高维生素A组（维生素A 250 IU/g）及对照组（维生素A 4 IU/g），每组10只，干预3周后应用流式细胞术测定小鼠外周血及肠黏膜CD4+ CD25+ T细胞亚群，应用ELISA法测定小鼠粪便中细胞因子IFN-γ、IL-4、IL-17及IL-23水平。结果：高维生素A组外周血及肠黏膜CD4+CD25+T细胞水平高于对照组(P<0.05)，粪便中IL-4水平高于对照组(P<0.05)，IL-23水平低于对照组(P<0.05)。结论：维生素A对小鼠外周血及小肠CD4+ CD25+ T细胞发育有促进作用,可能通过调节IL-4及IL-23等细胞因子参与肠黏膜相关免疫反应。［中国当代儿科杂志，2010，12(12)：976-978］

Abstract

OBJECTIVE: To study the effects of vitamin A on the development of T lymphocytes in peripheral blood and small intestine and on the cytokine response of intestinal mucosa in mice. METHODS: Twenty young mice were randomly fed with forage containing vitamin A 250 or 4 IU/g (n=10 each). Three weeks later, the levels of CD4+ CD25+ T subsets in peripheral blood and intestinal mucosa were measured by flow cytometry. The levels of cytokines IFN-γ, IL-4, IL-17 and IL-23 in stool were measured using ELISA. RESULTS: The levels of CD4+ CD25+ T subsets in peripheral blood and intestinal mucosa in the 250 IU/g vitamin A group were significantly higher than those in the 4 IU/g vitamin A group (P<0.05). The IL-4 level in stool increased, in contrast, the IL-23 level in stool decreased significantly in the 250 IU/g vitamin A group when compared with the 4 IU/g vitamin A group (P<0.05). CONCLUSIONS: vitamin A may promote the development of CD4+ CD25+ T lymphocytes in peripheral blood and small intestine. Moreover, it may be involved in intestinal mucosa-associated immune response by regulating cytokines IL-4 and IL-23.［Chin J Contemp Pediatr, 2010, 12 (12):976-978］

导出引用

杨凌云, 储波, 蒋丽蓉. 维生素A调节小鼠肠黏膜淋巴细胞免疫功能的作用[J]. 中国当代儿科杂志. 2010, 12(12): 976-978

YANG Ling-Yun, CHU Bo, JIANG Li-Rong. Effects of vitamin A on the immune function of intestinal mucosa lymphocytes in mice[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(12): 976-978

中图分类号： R-33

参考文献

［1］Strober W. Vitamin A rewrites the ABCs of oral tolerance［J］. Mucosal Immunol, 2008, 1(2): 92-95.
［2］Barnes MJ, Powrie F. Regulatory T cells reinforce intestinal homeostasis［J］. Immunity, 2009, 31(3): 401-411.
［3］Mottet C, Uhlig HH, Powrie F. Cutting edge: cure of colitis by CD4+ CD25+ regulatory T cells［J］. J Immunol, 2003, 170(8): 3939-3943.
［4］Kim CH. Roles of retinoic acid in induction of immunity and immune tolerance［J］. Endocr Metab Immune Disord Drug Targets, 2008, 8(4): 289-294.
［5］Kobayashi T, Okamoto S, Hisamatsu T, Kamada N, Chinen H, Saito R, et al. IL23 differentially regulates the Th1/Th17 balance in ulcerative colitis and Crohn′s disease［J］. Gut, 2008, 57(12):1682-1689.
［6］Dawson HD, Collins G, Pyle R, Key M, Weeraratna A, Deep-Dixit V, et al. Direct and indirect effects of retinoic acid on human Th2 cytokine and chemokine expression by human T lymphocytes［J］. BMC Immunol, 2006, 7:27.
［7］Xiao S, Jin H, Korn T, Liu SM, Oukka M, Lim B, etal. Retinoic acid increases Foxp3+ regulatory T cells and inhibits development of Th17 cells by enhancing TGF-beta-driven Smad3 signaling and inhibiting IL-6 and IL-23 receptor expression［J］. J Immunol, 2008, 181(4):2277-2284.