毛细支气管炎患儿ICAM-1和RANTES检测的意义

邹丽萍; 张松林

PDF(947 KB)

中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (3) : 181-183.

论著·临床研究

毛细支气管炎患儿ICAM-1和RANTES检测的意义

邹丽萍，张松林

作者信息 +

Detection of ICAM-1 and RANTES in infants with bronchiolitis

ZOU Li-Ping, ZHANG Song-Lin

Author information +

文章历史 +

摘要

目的：探讨毛细支气管炎（简称毛支炎）患儿细胞间黏附分子-1（ICAM-1）和激活调节正常T细胞表达和分泌因子（RANTES）检测的意义及相关性。方法：采用流式细胞术检测28例毛支炎患儿、23例支气管肺炎患儿及24例健康婴儿外周血淋巴细胞ICAM-1表达情况，ELISA法测定其血清RANTES水平，并进行相关性分析。结果：毛支炎组ICAM-1表达(35.0±10.3%)高于肺炎组（29.9±8.6%; P<0.05）及对照组（24.6±6.9%; P<0.01），肺炎组ICAM-1表达高于对照组（P<0.05）。毛支炎组和肺炎组RANTES水平分别为32.1±6.0和 30.6±6.2 ng/mL，高于对照组的27.1±5.1 ng/mL（分别P<0.01，<0.05），毛支炎组与肺炎组差异无统计学意义。ICAM-1与RANTES在毛支炎组呈正相关（r=0.675，P<0.01）。结论：ICAM-1和 RANTES参与了毛支炎的发病过程且具有协同作用。［中国当代儿科杂志，2010，12（3）：181-183］

Abstract

OBJECTIVE: To explore the role of intercellular adhesion molecule-1 (ICAM-1) and regulated upon activation normal T cell expressed and secreted (RANTES) in bronchiolitis and their correlation in the pathogenesis of this disorder. METHODS: The expression of ICAM-1 was detected by flow cytometry on lymphocytes of peripheral blood in 28 infants with bronchiolitis, 23 infants with bronchopneumonia and 24 healthy infants. Serum level of RANTES was assayed using ELISA. The correlation between ICAM-1 and RANTES levels was evaluated using Pearson correlation coefficient. RESULTS: The ICAM-1 level in the bronchiolitis group (35.0±10.3%) was much higher than that in the bronchopneumonia (29.9±8.6%; P<0.05) and the control groups (24.6±6.9%; P<0.01). The bronchopneumonia group had higher ICAM-1 level than the control group (P<0.05). The RANTES level in the bronchiolitis (32.1±6.0 ng/mL) and the bronchopneumonia groups (30.6±6.2 ng/mL) was significantly higher than that in the control group (27.1±5.1 ng/mL) (P<0.01, P<0.05, respectively), however, no significant difference was found between the bronchopneumonia and bronchiolitis groups. There was a positive correlation between ICAM-1 and RANTES levels in the bronchiolitis group (r=0.675, P＜0.01). CONCLUSIONS: ICAM-1 and RANTES are involved in the pathogenesis of bronchiolitis and show a synergistic effect.［Chin J Contemp Pediatr, 2010, 12 (3):181-183］

导出引用

邹丽萍, 张松林. 毛细支气管炎患儿ICAM-1和RANTES检测的意义[J]. 中国当代儿科杂志. 2010, 12(3): 181-183

ZOU Li-Ping, ZHANG Song-Lin. Detection of ICAM-1 and RANTES in infants with bronchiolitis[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(3): 181-183

中图分类号： R725.6

参考文献

［1］Reijonen TM, Kotaniemi-Syrjanen A, Korhonen K, Korppi M. Predictors of asthma three years after hospital admission for wheezing in infancy［J］. Pediatrics, 2000, 106(6):1406-1412．
［2］朱萍，邹丽萍.毛细支气管炎患儿外周血单个核细胞CD40/CD40L的表达［J］.中国当代儿科杂志，2009，11（5）：354-356.
［3］Kim HH, Lee MH, Lee JS. Eosinophil cationic protein and chemokines in nasopharyngeal secretions of infants with respiratory syncytial virus (RSV) bronchiolitis and non-RSV bronchiolitis［J］. J Korean Med Sci, 2007, 22(1):37-42.
［4］胡亚美，江载芳.诸福棠实用儿科学［M］.第7版.北京：人民卫生出版社，2002：1175-1201.
［5］Lai CC, Tai HY, Shen HD, Chung WT, Chung RL,Tang RB.Elevated levels of soluble adhesion molecules in sera of patients with acute bronchiolitis［J］.J Microbiol Immunol Infect, 2004, 37（3）:153-156.
［6］Arnold R, Konig W.Respiratory syncytial virus infection of human lung endothelial cells enhances selectively intercellular adhesion molecule-1 expression［J］．J Immunol, 2005, 174(3):7359-7367.
［7］陈叶,肖启亮,胡晓燕,邓莉莉.儿童毛细支气管炎血清sICAM-1和Eotaxin水平测定的临床意义［J］.南华大学学报·医学版，2008，36（1）：75-78.
［8］Uchida O, Kajiwara N, Hayashi A, Miyajima K, Nagatsuka T, Hayashi H, et al. Met-RANTES ameliorates fibrous airway obliteration and decreases ERK expression in a murine model of bronchiolitis obliterans［J］. Ann Thorac Cardiovasc Surg, 2007, 13（2）:82-86.
［9］陈小芳，楼永良，董琳,吕建新. 趋化因子RANTES在RSV感染中的表达及作用［J］.国外医学儿科学分册, 2005, 32(3):162-164.
［10］董琳，陈小芳，周晓聪，林剑，李锦燕，张伟．RSV毛细支气管炎RANTES MIP-lα IL-8水平变化及相关性研究［J］．浙江临床医学，2007，9(10)：1307-1308.
［11］Tian M, Liu F, Wen GY, Shi SY, Chen RH, Zhao DY. Effect of variation in RANTES promoter on serum RANTES levels and risk of recurrent wheezing after RSV bronchiolitis in children from Han, Southern China［J］. Eur J Pediatr, 2009, 168(8)：963-967.