人血丙种球蛋白和阿司匹林治疗对川崎病患儿循环内皮祖细胞功能的影响

徐明国,门丽娜,祖莹,赵春玉,孟祥春

中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (12) : 966-969.

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中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (12) : 966-969.
论著·临床研究

人血丙种球蛋白和阿司匹林治疗对川崎病患儿循环内皮祖细胞功能的影响

  • 徐明国,门丽娜,祖莹,赵春玉,孟祥春
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Effects of intravenous immunoglobulin and aspirin treatment on the functions of circulating endothelial progenitor cells in children with Kawasaki disease

  • XU Ming-Guo, MEN Li-Na, ZU Ying, ZHAO Chun-Yu, MENG Xiang-Chun
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摘要

目的:研究人血丙种球蛋白(IVIG)和阿司匹林(ASP)治疗对川崎病患儿循环内皮祖细胞功能的影响及其可能的机制。方法:抽取10例川崎病患儿IVIG和ASP治疗前及治疗7 d后的外周血,将单个核细胞诱导培养内皮祖细胞。利用MTT法、改良Boyden小室法、细胞培养板贴壁法测定内皮祖细胞的增殖、迁移和贴壁功能。同时测定血浆中肿瘤坏死因子-α(TNF-α)和超敏C反应蛋白(hs-CRP)的浓度。结果:IVIG和ASP治疗7 d后循环内皮祖细胞的功能较治疗前显著提高。IVIG和ASP治疗7 d后血浆中TNF-α和hs-CRP 的浓度显著降低。治疗后TNF-α和hs-CRP 浓度的降低与内皮祖细胞功能的提高呈显著正相关关系。结论:IVIG和ASP治疗可显著改善川崎病患儿循环内皮祖细胞的功能,其机制可能与TNF-α和hs-CRP的浓度降低有关。

Abstract

OBJECTIVE: To study the effects of intravenous immunoglobulin (IVIG) and aspirin treatment on the functions of circulating endothelial progenitor cells (EPCs) in children with Kawasaki disease (KD) and possible mechanisms. METHODS: Blood samples were obtained in 10 children with KD before and 7 days after the treatment by IVIG and aspirin. MTT method, modified Boyden chamber method and cell culture plate adhesion method were used to assess the functions of EPCs, including proliferation, adhension and migration activities. The plasma levels of tumor necrosis factor-α (TNF-α) and high-sensitivity C reactive protein (hs-CRP) were also measured. RESULTS: The functions of circulating EPCs 7 days after IVIG and aspirin treatment were significantly improved. IVIG and aspirin treatment significantly reduced plasma TNF-α and hs-CRP concentrations. There was a significant linear regression relationship between the reduced plasma TNF-α and hs-CRP levels and the increased functions of circulating EPCs.ConclusionsIVIG and aspirin treatment can improve the functions of circulating EPCs, possibly through reducing plasma concentrations of TNF-α and hs-CRP.

关键词

川崎病 / 内皮祖细胞 / 肿瘤坏死因子-α / 超敏C反应蛋白 / 儿童

Key words

Kawasaki disease / Endothelial progenitor cell / Tumor necrosis factor-α / High-sensitivity C reaction protein / Child

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徐明国,门丽娜,祖莹,赵春玉,孟祥春. 人血丙种球蛋白和阿司匹林治疗对川崎病患儿循环内皮祖细胞功能的影响[J]. 中国当代儿科杂志. 2011, 13(12): 966-969
XU Ming-Guo, MEN Li-Na, ZU Ying, ZHAO Chun-Yu, MENG Xiang-Chun. Effects of intravenous immunoglobulin and aspirin treatment on the functions of circulating endothelial progenitor cells in children with Kawasaki disease[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(12): 966-969
中图分类号: R725   

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