穿孔素和颗粒酶B在哮喘大鼠肺组织中的表达及重组人生长激素的干预作用

邹丽萍,杜文丽,张艳,王利霞

中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (3) : 223-226.

PDF(1297 KB)
PDF(1297 KB)
中国当代儿科杂志 ›› 2011, Vol. 13 ›› Issue (3) : 223-226.
论著·实验研究

穿孔素和颗粒酶B在哮喘大鼠肺组织中的表达及重组人生长激素的干预作用

  • 邹丽萍,杜文丽,张艳,王利霞
作者信息 +

Expression of perforin and granzyme B in asthmatic rats and intervention of recombinant human growth hormone

  • ZOU Li-Ping, DU Wen-Li, ZHANG Yan, WANG Li-Xia
Author information +
文章历史 +

摘要

目的:探讨哮喘大鼠肺组织中穿孔素(PFP)、颗粒酶B(GzmB)的表达变化及重组人生长激素(rhGH)的干预作用。方法:30只雄性Sprague-Dawley大鼠随机分为正常对照组、哮喘模型组及rhGH干预组。通过苏木精-伊红染色观察卵蛋白激发作用及rhGH干预后哮喘大鼠气道的病理变化。TUNEL原位凋亡检测支气管上皮细胞凋亡,RT-PCR方法检测肺组织中PFP及GzmB mRNA的表达。结果:哮喘组支气管上皮细胞凋亡指数明显增高,而rhGH干预组的凋亡指数则明显低于哮喘组(P<0.05);PFP和GzmB mRNA在对照组中呈低表达;在哮喘组的表达明显强于对照组(P<0.05);rhGH进行干预后,PFP和GzmB mRNA表达明显低于哮喘组(0.48±0.08 vs 0.63±0.08;0.44±0.13 vs 0.71±0.15;均P<0.05)。PFP和GzmB mRNA表达与支气管上皮细胞凋亡率呈正相关(r分别为 0.800 和 0.806,P<0.05)。结论:PFP和GzmB参与了哮喘发病过程中的重要环节;rhGH可能通过降低PFP和 GzmB的表达抑制支气管上皮细胞的凋亡从而抑制气道重塑。

Abstract

OBJECTIVE: To study the expression of perforin and granzyme B (GzmB) in the lungs of asthmatic rats and the effect of recombinant human growth hormone (rhGH) on the expression. METHODS: Thirty Sprague-Dawley male rats were randomly divided into a normal control group and asthma groups with and without rhGH treatment. An asthma model was prepared by repeated sensitization with ovalbumin and aluminium hydroxide. The morphological changes of the airway were observed by hematoxylin and eosin staining. Terminal deoxyribonucleotide transferase-mediated Dutp-bintin (TUNLE) was used to detect the apoptosis of epithelial cells in the airway. RT-PCR was used to detect the mRNA transcripts of perforin and GzmB in the lung tissues. RESULTS: A significantly increased apoptosis rate of airway epithelial cells was noted in the untreated asthma group. The apoptosis rate was significantly ruduced in the rhGH-treated asthma group (P<0.05). Compared with the control group, perforin and GzmB expression in the lungs in the untreated asthma group increased significantly. The rhGH-treated asthma group demonstrated significantly decreased perforin (0.48±0.08 vs 0.63±0.08; P<0.05) and GzmB (0.44±0.13 vs 0.71±0.15; P<0.05) expression in the lungs compared with the untreated asthma group. Both PFP (r=0.800, P<0.05) and GzmB (r=0.806, P<0.01) were positively correlated with the apoptosis rate of airway epithelial cells. CONCLUSIONS: Perforin and GzmB may play important roles in the pathogenesis of asthma. rhGH treatment can inhibit apoptosis of airway epithelial cells and airway remodeling, possibly through a reduction in perforin and GzmB expression.

关键词

哮喘 / 重组人生长激素 / 穿孔素 / 颗粒酶B / 大鼠

Key words

Asthma / Recombinant human growth hormone / Perforin / Granzyme B / Rats

引用本文

导出引用
邹丽萍,杜文丽,张艳,王利霞. 穿孔素和颗粒酶B在哮喘大鼠肺组织中的表达及重组人生长激素的干预作用[J]. 中国当代儿科杂志. 2011, 13(3): 223-226
ZOU Li-Ping, DU Wen-Li, ZHANG Yan, WANG Li-Xia. Expression of perforin and granzyme B in asthmatic rats and intervention of recombinant human growth hormone[J]. Chinese Journal of Contemporary Pediatrics. 2011, 13(3): 223-226
中图分类号: R-33   

参考文献

[1]Davies DE. The role of the epithelium in airway remodeling in asthma[J]. Proc Am Thorac Soc, 2009, 6(8): 678-682.

[2]郭春艳,韩波.穿孔素抗体对颗粒酶B在病毒性心肌炎中表达的影响[J].实用儿科临床杂志,2008,23(22):1754-1756.

[3]Pardo J, Wallich R, Ebnet K, Iden S, Zentgraf H, Martin P, et al.  Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin[J]. Cell Death Differ, 2007, 14(10): 1768-1779.

[4]毛海英,马敏,曹兰芳.哮喘患儿CD8+T淋巴细胞穿孔素与颗粒霉素B表达的研究[J]. 临床儿科杂志, 2008,26(12):1060-1062.

[5]Bratke K, B-ttcher B, Leeder K, Schmidt S, Küpper M, Virchow JC Jr, et al. Increase in granzyme B+lymphocytes and soluble granzyme B in bronchoalveolar lavage of allergen challenged patients with atopic asthma[J].Clin Exp Immunol, 2004, 136(3): 542-548.

[6]Kuwano K. Epithelial cell apoptosis and lung remodeling[J]. Cell Mol  Immunol, 2007,4(6):419-429.

[7]Benayoun L, Pretolani M. Airway remodeling in asthma: mechanisms and therapeutic perspectives[J]. Med Sci (Paris), 2003, 19(3): 319-326.

[8]舒玲华,陈新山.病毒性心肌炎猝死心肌Perforin和Desmin表达的研究[J].中华中西医杂志,2005,6(19):2587-2588.

[9]孙健,张建明,刘群,冯世海,施耘,赵永健. 重组人生长激素对烟雾吸入性损伤大鼠肺泡Ⅱ型细胞的增殖及 Bcl-2和 Bax蛋白表达的影响[J]. 中华损伤与修复杂志, 2008,3 (6):683-691.

[10]郑仰明,李昌崇,张维溪,管小俊.哮喘气道重塑大鼠气道上皮细胞凋亡及布地奈德的干预作用[J]. 中国循证科杂志,2006,1(2):106-112.

[11]孔祥永,栾斌,贾天明,冯建飞,王秀芳,王西阁,等.地塞米松对实验性哮喘大鼠气道细胞DNA合成和气道重塑的影响[J].中国当代儿科杂志,2002,4(2):109-113.

PDF(1297 KB)

Accesses

Citation

Detail

段落导航
相关文章

/