目的：观察黄芩苷（BAC）对自身免疫性脑脊髓炎（EAE）大鼠的疗效及其对脊髓炎症细胞凋亡的影响。方法：44 只Wistar大鼠随机分为正常对照组（NC, n=10）、EAE 组(n=12)、地塞米松治疗组（DXM, n=12）、BAC 治疗组(n=10)。 DXM 组和 BAC 组在抗原免疫后1周分别予以DXM (1 mg/kg) 和BAC (200 mg/kg) 治疗7 d；观察各组动物脊髓病理变化及髓鞘碱性蛋白（MBP）表达的情况和脊髓炎症细胞凋亡情况。结果：EAE 组、DXM 组和 BAC 组较NC组体重均下降，BAC 组体重较 EAE 组及DXM组明显上升（P<0.05）。DXM 组和 BAC 组大鼠神经功能评分明显优于EAE组（P<0.05）；DXM 组和 BAC 组脊髓 MBP 阳性数均较 EAE 组显著增多（P<0.05）。DXM 组和 BAC 组脊髓炎症细胞凋亡数均较EAE 组显著增多（P<0.05）。 结论：BAC对EAE大鼠有治疗作用，并且能促进大鼠脊髓炎症细胞凋亡。
Abstract
OBJECTIVE: To study the therapeutic efficacy of baicalin and its effect on apoptosis of inflammatory cells in spinal cords in Wistar rats with autoimmune encephalomyelitis (EAE). METHODS: Forty-four rats were randomly divided into four groups: normal control group (control, n=10), EAE group (n=12), and two intervention groups with dexamethasone (DXM) or baicalin. Seven days after immunization, the two intervention groups were injected intraperitoneally with DXM (1 mg/kg) and baicalin (200 mg/kg) for 1 week, respectively. The spinal cords were removed 14 days after immunization, and stained with hematoxylin and eosin. MBP expression in spinal cords was detected by immunohistochemistry. The apoptosis of inflammatory cells in spinal cords was detected by TUNEL. RESULTS: The weight gain rate in the untreated EAE and the DXM or baicalin intervention groups were significantly lower than that in the control group (P<0.05). The weight gain rate in the baicalin intervention group was significantly higher than that in the untreated EAE and the DXM intervention groups (P<0.05). The scores of neurological function in the two intervention groups were significantly higher than that in the untreated EAE group (P<0.05). DXM or baicalin treatment significantly increased the MBP expression compared with the untreated EAE group (P<0.05). The apoptosis of inflammatory cells increased more in the DXM and the baicalin intervention groups compared with the untreated EAE groups (P<0.05). CONCLUSIONS: Baicalin has protective effects against EAE in rats. It can promote the apoptosis of inflammatory cells in spinal cords.
关键词
实验性自身免疫性脑脊髓炎 /
细胞凋亡 /
地塞米松 /
黄芩苷 /
大鼠
Key words
Autoimmune encephalomyelitis /
Apoptosis /
Dexamethasone /
Baicalin /
Rats
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参考文献
［1］Bauer J , Wekerle H, Lassmann H. Apoptosis in brain-specific autoimmune disease［J］. Curr Opin Immunol, 1995, 7(6): 839-843.
［2］Nguyen KB, McCombe PA , Pender MP. Increase apoptosis of T lymphocytes and macrophages in the central and peripheral nervous systems of Lewis rats with experimental autoimmune encephalomyelitis treated with dexamethasone［J］. J Neuropathol Exp Neurol, 1997, 56(1): 58-69.
［3］Chao JI, Su WC, Liu HF. Baicalein induces cancer cell death and proliferation retardation by the inhibition of CDC2 kinase and survivin associated with opposite role of p38mitogen-activated protein kinase and AKT Mol［J］. Cancer Ther, 2007, 6(11): 3039-3048.
［4］匡菊香,雷沛鸿 ,石江.黄芩苷对人黑色素瘤A375细胞增殖的影响［J］.中华中医药学刊，2008,26(11):2510-2512.
［5］Zhang XP, Tian H, Lai YH, Chen L, Zhang L, Cheng QH, et al. Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis［J］. World J Gastroenterol, 2007,13（38）：5079-5089.
［6］曹翠丽,王惠,马常升,杨天祝．实验性变态反应性脑脊髓炎大鼠模型的建立［J］.解剖学杂志,2001,24(1 ):77-80.
［7］Maccioni M, Riera CM, Rivero VE. Peritoneal antigen-presenting cells pulsed in vivo with myelin basic protein induce the suppression of experimental autoimmune encephalomyelitis (EAE) in Wistar rats［J］. J Neuroimmunol, 1999, 96（1）:46-56.
［8］陈慧芳,吕佩源,陈竞清,林嘉友.EAE大鼠中枢神经系统与外周免疫器官细胞凋亡的研究［J］.中国神经免疫学和神经病学杂志,2004,11(5):280-283.
［9］McCombe PA, Nickson I, Tabi Z, Pender MP. Corticosteroid treatment of experimental autoimmune encephalomyelitis in the Lewis rat results in loss of V beta 8.2+ and myelin basic protein-reactive cells from the spinal cord, with increased total T-cell apoptosis but reduced apoptosis of V beta 8.2+ cells［J］. J Neuroimmunol, 1996, 70(2): 93-101.
［10］林小娟,杨于嘉, 祁伯祥,王霞,宋健辉.黄芩苷预处理对新生大鼠缺氧缺血性脑损伤的保护作用［J］. 中国当代儿科杂志，2006, 8 (3) : 221-224.
［11］朱伟,孙红光,朱迅.黄芩苷元对炎症反应的影响［J］.中国药理学通报,2009,25(2):194-197.
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