Abstract：OBJECTIVE: To measure levels of interleukin-4 (IL-4) and interferon-gamma (INF-γ) in the bronchoalveolar lavage fluid (BALF) of children with refractory Mycoplasma pneumoniae pneumonia (RMPP), and to investigate changes in local Th1-Th2-type cytokine levels in children with RMPP and their significance. METHODS: A total of 42 children with RMPP were divided into atopic (n=11) and non-atopic groups (n=31) according to whether they had eczema, allergic rhinitis, urticaria, and family history of allergic disease. The study also included a control group of 12 children with bronchial foreign bodies who underwent foreign body removal and were re-examined by fiberoptic bronchoscopy four weeks later. The different cells in BALF from all children were analyzed, and the levels of IL-4 and INF-γ in BALF were measured using enzyme-linked immunosorbent assay. RESULTS: Compared with the control group, the total number of cells in BALF from children with RMPP increased significantly (P0.05). CONCLUSIONS: Significant increase in cell numbers, especially neutrophils, as well as IL-4 and INF-γ levels, can be seen in BALF from children with RMPP, but there is no change to the INF-γ/IL-4 ratio. This indicates a significant local inflammatory response in children with RMPP, but there is no evidence of Th2-dominated inflammatory response.
HUANG Han,ZHONG Li-Li,PENG Li et al. Levels of interleukin-4 and interferon-gamma in the bronchoalveolar lavage fluid of children with refractory Mycoplasma pneumoniae pneumonia[J]. CJCP, 2012, 14(08): 581-584.
［1］Ou CY, Tseng YF, Chiou YH, Nong BR, Huang YF, Hsieh KS. The role of Mycoplasma pneumoniae in acute exacerbation of asthma in children［J］. Acta Paediatr Taiwan, 2008, 49(1): 14-18.
［2］Kraft M, Cassell GH, Henson JE, Watson H, Williamson J, Marmion BP, et al. Detection of Mycoplasma pneumoniae in the airways of adults with chronic asthma［J］. Am J Respir Crit Care Med, 1998, 158(3): 998-1001.
［3］Waites KB, Balish MF, Atkinson TP. New insights into the pathogenesis and detection of Mycoplasma pneumoniae infections［J］. Future Microbiol, 2008, 3(6): 635-648.
［4］Waites KB. New concepts of Mycoplasma pneumonia infections in children［J］. Pediatric Pulmonol, 2003, 36(4): 267-278.
［8］Stelmach I, Podsiadlowicz-Borzecka M, Grzelewski T, Majak P, Stelmach W, Jerzyńska J, et al. Humoral and cellular immunity in children with Mycoplasma pneumoniae infection: a 1-year prospective study［J］. Clin Diagn Lab Immunol, 2005, 12(10): 1246-1250.
［12］Koh YY, Park Y, Lee HJ, Kim CK. Levels of interleukin-2, interferon-g, and interleukin-4 in bronchoalveolar lavage fluid from patients with mycoplasma pneumonia: implication of tendency toward increased immunoglobulin E production［J］. Pediatrics, 2001, 107(3): E39.
［14］Choi IS, Byeon JH, Yoo Y, Lee KC, Choung JT. Increased serum interleukin5 and vascular endothelial growth factor in children with acute mycoplasma pneumonia and wheeze［J］. Pediatr Pulmonol, 2009, 44(5): 423-428.
［16］Chu HW, Honour JM, Rawlinson CA, Harbeck RJ, Martin RJ. Effects of respiratory Mycoplasma pneumoniae infection on allergen-induced bronchial hyperresponsiveness and lung inflammation in mice［J］. Infect Immun, 2003, 71(3): 1520-1526.
