miRNA-126/miRNA-126*在大鼠正常胎肺发育过程中的作用

杨洋; 阚清; 邱洁; 浦晓丹; 张盼; 张晓群; 周晓玉

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中国当代儿科杂志 ›› 2012, Vol. 14 ›› Issue (09) : 708-712.

论著·实验研究

miRNA-126/miRNA-126*在大鼠正常胎肺发育过程中的作用

杨洋，阚清，邱洁，浦晓丹，张盼，张晓群，周晓玉

作者信息 +

Role of miRNA-126/miRNA-126* in the fetal lung development of rats

YANG Yang, KAN Qing, QIU Jie, PU Xiao-Dan, ZHANG Pan, ZHANG Xiao-Qun, ZHOU Xiao-Yu

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文章历史 +

摘要

目的：探讨miRNA-126/miRNA-126*在大鼠胎肺发育中的表达水平及其作用。方法：12只孕鼠随机分为3组，每组4只，3组仔鼠分别于胎龄16 d、19 d组和21 d时取出，运用苏木精-伊红染色在仔鼠胎肺发育的这3个关键时期对胎肺进行形态学观察，随后通过建立microRNA（miRNA）芯片，了解miRNA-126/miRNA-126*在3组仔鼠胎肺中的基本表达趋势，进而挑选出miRNA-126*并应用real-time PCR对其表达水平行进一步研究。结果：miRNA-126在3组芯片间表达无明显差异，而miRNA-126*随着肺发育进程表达量逐渐上升；采用real-time PCR对miRNA-126*表达进行进一步检测，结果亦显示miRNA-126*随着肺发育进程表达量逐渐上升，组间两两比较差异均有统计学意义（P<0.05）。结论：miRNA-126*可能在大鼠胎肺发育中起着重要作用。

Abstract

OBJECTIVE: To investigate the expression and role of miRNA-126/miRNA-126* in the fetal lung development of rats. METHODS: Twelve pregnant Sprague-Dawley rats were randomly divided into 3 groups and the fetal rats were removed at 16, 19 and 21 days of gestation respectively. Hematoxylin and eosin staining was performed to observe lung morphology of fetal rats. Then microRNA (miRNA) microarray was used to study the expression patterns of miRNA-126/miRNA-126* in fetal lungs at the three time points. And miRNA-126* was selected for further study by real-time PCR. RESULTS: There was no evident difference in the expression of miRNA-126 among the three groups, however the expression level of miRNA-126* increased gradually as the fetal lung developed. The real-time PCR result further showed that expression of miRNA-126* increased gradually with lung development, displaying significant differences among the three groups (P<0.05). CONCLUSIONS: miRNA-126* may play an important role in development of the fetal lung in rats.

导出引用

杨洋，阚清，邱洁，浦晓丹，张盼，张晓群，周晓玉. miRNA-126/miRNA-126*在大鼠正常胎肺发育过程中的作用[J]. 中国当代儿科杂志. 2012, 14(09): 708-712

YANG Yang, KAN Qing, QIU Jie, PU Xiao-Dan, ZHANG Pan, ZHANG Xiao-Qun, ZHOU Xiao-Yu. Role of miRNA-126/miRNA-126* in the fetal lung development of rats[J]. Chinese Journal of Contemporary Pediatrics. 2012, 14(09): 708-712

中图分类号： R-33

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