Department of Pediatrics, Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China. xrguo@njmu.edu.cn
Abstract：MicroRNA (miRNA) is a class of non-coding endogenous small molecule single strand RNA which is found in human body fluids. In recent years, miRNAs have been found in breast milk and parts of miRNAs are related to immune organ development and regulation of the immune function in infants. This article summarizes the functions of miRNA in breast milk and evidence-based clinical practice, and the differences between microRNA content and species in breast milk and cow milk. Understandng the role of miRNA can bring new opportunities for childhood nutrition research.
［1］Gibson RA, Neumann MA, Makrides M. Effect of increasing breast milk docosahexaenoic acid on plasma and erythrocyte phospholipid fatty acids and neural indices of exclusively breast fed infants［J］. Eur J Clin Nutr, 1997, 51(9): 578-584.
［2］Sokolov AV, Pulina MO, Zakharova ET, Susorova AS, Runova OL, Kolodkin NI, et al. Identification and isolation from breast milk of ceruloplasmin-lactoferrin complex［J］. Biochemistry (Mosc), 2006, 71(2): 160-166.
［3］Rodriguez A, Raederstorff D, Sarda P, Lauret C, Mendy F, Descomps B. Preterm infant formula supplementation with alpha linolenic acid and docosahexaenoic acid［J］. Eur J Clin Nutr, 2003, 57(6): 727-734.
［4］Yen MH, Chiu CH, Huang YC, Lin TY. Effects of lactoferrin-containing formula in the prevention of enterovirus and rotavirus infection and impact on serum cytokine levels: a randomized trial［J］. Chang Gung Med J, 2011, 34(4): 395-402.
［5］Marchbank T, Weaver G, NilsenHamilton M, Playford RJ. Pancreatic secretory trypsin inhibitor is a major motogenic and protective factor in human breast milk［J］. Am J Physiol Gastrointest Liver Physiol, 2009, 296(4):G697-G703.
［6］Mossberg AK, Puchades M, Halskau O, Baumann A, Lanekoff I, Chao Y, et al. HAMLET interacts with lipid membranes and perturbs their structure and integrity［J］. PLoS One, 2010, 5(2): e9384.
［7］Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14［J］. Cell, 1993, 75(5): 843-854.
［8］Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans［J］. Nature, 2000, 403(6772): 901-906.
［9］Ruvkun G. Molecular biology. Glimpses of a tiny RNA world［J］. Science, 2001, 294(5543): 797-799.
［11］Krol J, Loedige I, Filipowicz W. The widespread regulation of microRNA biogenesis, function and decay［J］. Nat Rev Genet, 2010, 11(9): 597-610.
［12］Sandhu S, Garzon R. Potential applications of microRNAs in cancer diagnosis, prognosis, and treatment［J］. Semin Oncol, 2011, 38(6): 781-787.
［13］Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, et al. The microRNA spectrum in 12 body fluids［J］. Clin Chem, 2010, 56(11): 1733-1741.
［14］Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases［J］. Cell Res, 2008, 18(10): 997-1006.
［15］Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection［J］. Proc Natl Acad Sci U S A, 2008, 105(30):10513-10518.
［16］Park NJ, Zhou H, Elashoff D, Henson BS, Kastratovic DA, Abemayor E, et al. Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection［J］. Clin Cancer Res, 2009, 15(17): 5473-5477.
［17］Hanke M, Hoefig K, Merz H, Feller AC, Kausch I, Jocham D, et al. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer［J］. Urol Oncol, 2010, 28(6): 655-661.
［18］Montenegro D, Romero R, Pineles BL, Tarca AL, Kim YM, Draghici S, et al. Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes［J］. Am J Obstet Gynecol, 2007, 197(3): 289.e1-6.
［19］Iguchi H, Kosaka N, Ochiya T. Secretory microRNAs as a versatile communication tool［J］. Commun Integr Biol, 2010, 3(5): 478-481.
