Abstract：OBJECTIVE: Suppressors of cytokine signaling (SOCS) have been shown to play an important role in regulating cytokines, such as intracellular interferon (IFN) and interleukin (IL), in the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway. At present, the association between SOCS and asthma is still under study. The aim of this study is to explore the relationship of SOCS-1 and SOCS-3 mRNA expression in peripheral blood mononuclear cells (PBMCs) with the intracellular IFN-γ/IL-4 ratio in CD4+ T cells and specific IgE (sIgE) level in children with asthma. METHODS: BMCs were collected from 44 children with allergic asthma (4-14 years) and 30 healthy children. The intracellular IFN-γ/IL-4 ratio in CD4+ T cells was measured by flow cytometry. Total RNAs were extracted from the PBMCs and SOCS-1 and SOCS-3 mRNA expression was measured by SYBR Green I quantitative RT-PCR. RESULTS: Compared with the healthy children, children with allergic asthma showed a lower level of intracellular IFN-γ in peripheral blood ［(15.7 ± 2.0)% vs (19.1 ± 2.7)%］ and IFN-γ/IL-4 ratio (3.4 ± 1.5 vs 4.8 ± 2.9) and higher SOCS-1 mRNA expression (-Ct, 11.1 ± 1.9 vs 12.6 ± 2.8）.There was a negative relationship between SOCS-1 mRNA expression and the percentage of IFN-γ-producing CD4+ T cells in peripheral blood in both asthmatic and healthy children (P<0.05). No correlation was found between SOCS-1 and SOCS-3 expression and sIgE level. CONCLUSIONS: Children with allergic asthma have elevated levels of SOCS-1 mRNA in PBMCs, which is associated with Th2-skewed immune response.
HUI Yu,XIE Juan-Juan,LI Ling et al. Association between suppressors of cytokine signaling mRNA expression and Th1/Th2 balance in children with asthma[J]. CJCP, 2012, 14(10): 755-758.
［3］Knisz J, Rothman PB. Suppressor of cytokine signaling in allergic Inflammation［J］. J Allergy Clin Immunol, 2007, 119(3): 739-745.
［4］Egwuagu CE, Yu CR, Zhang M, Mahdi RM, Kim SJ, Gery I. Suppressors of cytokine signaling proteins are differentially expressed in Th1 and Th2 cells: implications for Th cell lineage commitment and maintenance［J］. J Immunol, 2002,168(7): 3181-3187.
［7］Daegelmann C, Herberth G, Roder S, Herbarth O, Giese T, Krmer U, et al. Association between suppressors of cytokine signalling, T-helper type 1/T-helper type 2 balance and allergic sensitization in children［J］. Clin Exp Allergy, 2008, 38(3): 438-448.
［8］Yoshimura A, Naka T, Kubo M. SOCS proteins, cytokine signalling and immune regulation［J］. Nat Rev Immunol, 2007, 7(6): 454-465.
［9］Cassel SL, Rothman PB. Chapter 3: Role of SOCS in allergic and innate immune responses［J］. Adv Immunol, 2009, 103: 49-76.
［10］Fukuyama S, Nakano T, Matsumoto T, Oliver BG, Burgess JK, Moriwaki A, et al. Pulmonary suppressor of cytokine signaling-1 induced by IL-13 regulates allergic asthma phenotype［J］. Am J Respir Crit Care Med, 2009, 179(11): 992-998.
［11］Seki Y, Inoue H, Nagata N, Hayashi K, Fukuyama S, Matsumoto K, et al. SOCS-3 regulates onset and maintenance of T(H)2-mediated allergic responses［J］. Nat Med, 2003, 9(8): 1047-1054.
