目的：探讨哮喘免疫调节基因Tim-4启动子区和内含子区多态性与中国西南地区儿童变应性哮喘的遗传易感性的关联。方法：选取哮喘组儿童579例，平均年龄7.2岁；对照组儿童524例，平均年龄7.6岁。研究TIM-4基因启动子区的RS6882076和内含子区RS4704727两个位点，采用PCR-RFLP检测所有研究对象的两个多态性位点的基因型，进行病例对照研究分析。结果：PCR-RFLP检测结果显示，TIM-4基因启动子区的RS6882076和内含子区RS4704727这两个多态性位点在检测人群中均存在。哮喘组和对照组RS4704727位点基因型分布频率差异有统计学意义（P0.05）。结论：TIM-4基因内含子区RS4704727位点与儿童变应性哮喘易感性相关，为中国西南地区儿童哮喘的发病机制研究提供了参考。
Abstract
OBJECTIVE: To elucidate whether the polymorphism of asthma immune regulator gene TIM-4 is associated with the risk of childhood allergic asthma in the southwest region of China. METHODS: TIM-4 gene promoter region RS6882076 and intron RS4704727 were studied. PCR-RFLP was used to test the genotypes of two polymorphism loci among 579 cases (average 7.2 years old) of asthma and 524 controls (average 7.6 years old) in a case-control study. RESULTS: There were significant differences in the frequency of gene types at RS4704727 site between the asthma and the control groups (P0.05). CONCLUSIONS: RS4704727 polymorphism of TIM-4 gene may be associated with childhood asthma, providing a better understanding of the pathogenesis of childhood asthma in the Southwest region of China.
关键词
哮喘 /
单核苷酸多态性 /
TIM-4 /
儿童
Key words
Asthma /
Single nucleotide polymorphism /
TIM-4 /
Child
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