Effect of human cytomegalovirus on proliferation of hematopoietic progenitor cells of cord blood
LIU Wen-Jun, JIN Run-Ming, FU Xiao-Dong, LIU Bin, GUO Qu-Lian, DENG Zheng-Hua
Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430022, China. lwjlyfy@yahoo.com.cn
Abstract：OBJECTIVE: This study was designed to investigate the effect of human cytomegalovirus(HCMV) on the proliferation of colony forming unit granulocyte-macrophage(CFU-GM),CFU-erythroid(CFU-E),burst forming unit-erythroid(BFU-E),CFU-multipotential(CFU-Mix) and CFU-megakaryocytic(CFU-Mk) progenitor cells of cord blood in vitro as well as the possible mechanism. METHODS: Twenty cord blood specimens were collected from the umbilical vein of normal full-term neonates delivered spontaneously.This study consisted of five groups: 3 Infection groups in which 0.1 mL 10~3,10~4 and 10~5 plague forming unit(PFU) HCMV-AD_(169) virus solution was added to the culture system,an Inactivated control group in which the equal volume of inactivated virus solution was added,and a Blank control group(normal progenitor cells culture system without HCMV virus infection).Colony forming unit-assay was applied to detect the effects of HCMV-AD_(169) strain on the colony formation,inhibition rate and colony-maintaining duration of CFU-GM,CFU-E,BFU-E,CFU-Mix and CFU-Mk of cord blood.PCR technique was used to demonstrate the existence of HCMV-DNA in the colony cells of cultured CFU-GM,CFU-E,CFU-Mix and CFU-Mk. RESULTS: HCMV-AD_(169)(10~3 PFU) in low concentration had inhibition effects on colony formation of the CFU-Mix and CFU-MK(P<0.05),whereas 10~5 PFU and 10~4 PFU HCMV-AD_(169) lead to decreased colonies in CFU-GM,CFU-E,BFU-E,CFU-Mix and CFU-MK compared with the Blank control and the Inactivated control groups(P<0.05).The suppression effect of HCMV on the colony formation was dose-dependant.The colony-maintaining duration of the CFU-GM,CFU-E,BFU-E,CFU-Mix and CFU-Mk in the 10~5 PFU and 10~4 PFU HCMV infection groups was significantly shorter than that in the two control groups(P<0.01).The low concentration of HCMV-AD_(169)(10~3 PFU) infection resulted in a shortened colony-maintaining duration of the CFU-Mix and CFU-Mk(P<0.01),but had no effects on the colony-maintaining duration of CFU-GM,CFU-E and BFU-E.PCR amplification demonstrated the existence of HCMV-AD_(169) DNA in the colony cells of the three Infection groups. CONCLUSIONS: HCMV-AD_(169) strain can infect hematopoietic progenitors of cord blood and inhibit the proliferation of hematopoietic progenitors,associated with anemia,neutropenia and thrombocytopenia in HCMV patients.
LIU Wen-Jun,JIN Run-Ming,FU Xiao-Dong et al. Effect of human cytomegalovirus on proliferation of hematopoietic progenitor cells of cord blood[J]. CJCP, 2006, 8(2): 85-89.
