Abstract：Objective To study the effects of umbilical cord monoculcear cells (UCBMC) transplantation combined with hyperbaric oxygen (HBO) therapy on the long-term behaviors and histology in neonatal rats after hypoxic-ischemic brain damage (HIBD). Methods Seven-day-old Sprague-Dawley rats were randomly assigned to four groups: normal control (CON), HIBD, UCBMC and UCBMC+HBO. HIBD was induced according to the Rice-Vannucci method. The rats in the UCBMC+HBO group were treated with HBO 3 hours after HIBD, followed by UCBMC transplantation 24 hours after HIBD. IL-1β and TNF-α protein levels were examined by Western blot analysis in the 4 groups. T-maze test and radial arm maze test were used to detect the long-term learning memory capability. Nissl staining was used to examine the histological changes of the hippocampal CA1 region. Results Twenty-four hours after transplantation, IL-1β and TNF-α protein levels in the UCBMC+HBO group were significantly reduced compared with the HIBD (P<0.01) and UCBMC groups (P<0.05). The study and memory capabilities were impaired, and the number of the pyramidal cells in the hippocampal CA1 region was reduced in the HIBD group. The study and memory capabilities were greatly improved and the number of pyramidal cells increased significantly in the UCBMC+HBO group compared with the UCBMC and HIBD groups (P<0.05). Conclusions UCBMC transplantation combined with HBO therapy could reduce the expression of IL-1β and TNF-α protein, improve long-term behaviors and alleviate brain damages in the hypoxic ischemic neonatal rats.
MA Ze,YAN Shao-Zhen,WANG Xiao-Li et al. Effects of umbilical cord mononuclear cells transplantation combined with hyperbaric oxygen therapy on hypoxic-ischemic brain damage in neonatal rats[J]. CJCP, 2015, 17(7): 736-740.
Wang XL, Zhao YS, Hu MY, et al. Umbilical cord blood cells regulate endogenous neural stem cell proliferation via hedgehog signaling in hypoxic ischemic neonatal rats[J]. Brain Res, 2013, 1518: 26-35.
[3]
Yang YJ, Wang XL, Yu XH, et al. Hyperbaric oxygen induces endogenous neural stem cells to proliferate and differentiate in hypoxic-ischemic brain damage in neonatal rats[J]. Undersea Hyperb Med, 2008, 35(2): 113-129.
[4]
Wang Y, Zhang S, Luo M, et al. Hyperbaric oxygen therapy improves local microenvironment after spinal cord injury[J]. Neural Regen Res, 2014, 9(24): 2182-2188.
[5]
Qian L, Shen J, Zhao D, et al. Successful treatment of hemorrhagic cystitis after HLA-mismatched allogeneic hematopoietic stem cell transplantation by hyperbaric oxygen[J]. Transplantation, 2014, 97(7): e41-e42.
Wang X, Zhang J, Si D, et al. Progesterone inhibits the expression of cycloxygenase-2 and interleukin-1β in neonatal rats with hypoxic ischemic brain damage[J]. Int J Neurosci, 2014, 124(1): 42-48.
[10]
Wang XL, Zhao YS, Yang YJ, et al. Therapeutic window of hyperbaric oxygen therapy for hypoxic-ischemic brain damage in newborn rats[J]. Brain Res, 2008, 1222: 87-94.
[11]
Zhu Y, Guan YM, Huang HL, et al. Human umbilical cord blood mesenchymal stem cell transplantation suppresses inflammatory responses and neuronal apoptosis during early stage of focal cerebral ischemia in rabbits[J]. Acta Pharmacol Sin, 2014, 35(5): 585-591.
[12]
Stigger F, Lovatel G, Marques M, et al. Inflammatory response and oxidative stress in developing rat brain and its consequences on motor behavior following maternal administration of LPS and perinatal anoxia[J]. Int J Dev Neurosci, 2013, 31(8): 820-827.
[13]
Chen LF, Tian YF, Lin CH, et al. Repetitive hyperbaric oxygen therapy provides better effects on brain inflammation and oxidative damage in rats with focal cerebral ischemia[J]. J Formos Med Assoc, 2014, 113(9): 620-628.
[14]
Lee YS, Chio CC, Chang CP, et al. Long course hyperbaric oxygen stimulates neurogenesis and attenuates inflammation after ischemic stroke[J]. Mediators Inflamm, 2013, 2013: 512978.
