Abstract：OBJECTIVE: It was aimed to determine the concentration of urine 8-iso-PGF_ 2α in normal neonates and neonates with hypoxic-ischemic encephalopathy (HIE) and to study the value of urine 8-iso-PGF_ 2α in the assessment of the severity of neonatal HIE. METHODS: Urine samples from normal (n=126) and HIE (n=151) neonates were collected on the 1st, 3rd and 7th days after birth. ELISA was used to determine the urine 8-iso-PGF_ 2α contents. RESULTS: 1) Urine 8-iso-PGF_ 2α contents from normal neonates were 29.9 ± 7.9, 27.7 ± 9.8 and 27.5 ± 10.5 ng/mmol·Cr on the 1st, 3rd and 7th days after birth, respectively. There was no significant difference among the three days for the urine 8-iso-PGF_ 2α contents. 2) On the 1st day of HIE onset, neonates with mild, moderate and severe HIE had higher levels of urine 8-iso-PGF_ 2α (65.3 ± 13.2, 154.6 ± 31.6 and 241.7 ± 41.0 ng/mmol·Cr, respectively) compared with the normal neonates (P﹤0.001 ). 3) On the 3rd day, the urine 8-iso-PGF_ 2α content in neonates with moderate and severe HIE remained higher (34.2 ± 10.3 and 50.8 ± 12.8 ng/mmol·Cr, respectively) than the normal neonates (P﹤0.001 ), while that of the neonates with mild HIE regressed to normal. 4) On the 7th day, there was no significant difference in the urine 8-iso-PGF_ 2α level between all HIE and normal neonates. 5) The boundaries of 45.5, 89.9 and 217.5 ng/mmol·Cr of urine 8-iso-PGF_ 2α were defined to distinguish from normal to mild HIE, from mild to moderate HIE and from moderate to severe HIE. As defined, the corresponding sensitivity and specificity were 95.2% and 99.2%, 100% and 95.2%, 65.8% and 100%. CONCLUSIONS: The urine 8-iso-PGF_ 2α levels in neonates were stable within 7 days after birth. The urine 8-iso-PGF_ 2α contents in HIE neonates reached the peak within 24 hours after onset. There was a correlation between the urine 8-iso-PGF_ 2α contents and the severity of HIE, suggesting that urine 8-iso-PGF_ 2α may be a reliable and convenient index for the assessment of the severity of HIE.
CHEN Ling-Ling,XIAO Xin,XIONG Ai-Hua et al. Value of urine 8-iso-PGF2αin the assessment of the severityof neonatal hypoxic-ischemic encephalopathy[J]. CJCP, 2005, 7(2): 103-106.
