Relationship between telomerase activity and expression of p15INK4b gene in children with acute leukemia
WEN Fei-Qiu, CHEN Yi-Xin, CAO Li-Zhi, LIU Feng, ZHOU Ke-Ying, CHEN Ting
Department of Pediatrics, Second Affiliated Hospital, Shenzhen People Hospital, Jinan Medical College,Jinan Univesity, Shenzhen,Guangdong 518020, China
Abstract：OBJECTIVE: To study the relationship between telomerase activity and p15INK4b (p15) expression in the development of childhood acute leukemia (AL). METHODS: Telomerase activity and p15 expression in bone marrow (BM) of 27 cases of childhood AL were evaluated using a modified telomeric repeat amplification protocol assay and reverse-polymerase chain reaction respectively. For comparative analysis, BM from control donors (n = 9) were analyzed. RESULTS: At diagnosis, the telomerase activity detected in BM from AL children was significantly higher than that from control donors (34.5±37.0 TPG vs 2.4±2.2 TPG, P < 0.001). The p15 expression in BM of AL children was significantly lower than that of the controls (9.8±16.2% vs 45.8±16.9%, P < 0.001). No significant correlation was observed between p15 expression and telomerase activity (r = - 0.01304, P >0.05). CONCLUSIONS: The telomerase up-regulation and lower expression of p15 gene may play an important role in the development of AL by different mechanisms.
WEN Fei-Qiu,CHEN Yi-Xin,CAO Li-Zhi et al. Relationship between telomerase activity and expression of p15INK4b gene in children with acute leukemia[J]. CJCP, 2005, 7(2): 112-114.
