OBJECTIVE: To study the changes of serum TNF-α and NO in neonatal rats with endotoxic shock and its relationship with myocardial cells damge and to explore the protection effect of dexamethasone (DXM) on neonatal rats with endotoxic shock. METHODS: Nine of the 117 seven-day-old healthy neonatal Wistar rats were used as the pre-experimental base value control group, and the other 108 rats were randomly divided into control group, endotoxic shock group (LPSgroup, LPS 5 nig/kg) and treatment group (DXM group, LPS 5 mg/kg + DXM 5 mg/kg). Before and after injection of LPS (2, 4, 6 and 24 hs), 9 rats in each group were sacrificed and blood samples were collected to detect TNF-a by ELISA and NO by nitrate reductase. Myocardial super-microstructure was observed under an electron microscope. RESULTS: ① In the LPS group, the concentration of serum TNF-α peaked at 2 h, and it decreased to the level of control group after 6 hs. In the DXM group, the level of TNF-α at 2 h was higher than that of control group ( P < 0.05), but lower than that of the LPS group ( P < 0.05). At 4 hs the level TNF-α in DXM group was lower than that of the LPS group ( P <0.05), but was not different than that of the control group ( P >0.05). From 6 h after injection, the differences of TNF-α levels in 3 groups were not obvious ( P >0.05). ② In the LPS group, the concentration of NO rose after 2 h ( P <0. 01), and peaked at 24 h ( P <0. 01). After 2 hs, the levels of NO in the DXM group were lower than those of the LPS group ( P < 0.01). ③ Six hours after the injection of LPS, a little mitochondria of myocardial cells in the LPS group appeared to develop vacuolae-like degeneration. At 24 h, most mitochondria of myocardial cells in the LPS group presententecl with vaculea-like degeneration and necrosis. The myocardial fibers were broken. While in the DXM group, the changes in the super-microstructure at 24 h were not as serious as those which talk place in the LPS group. CONCLUSIONS: TNF-α and No were involved in the damage of myocardial cells in neonatal rats with endotoxic shock. DXM could partly protect the myocardial cells from damage by inhibiting the production of TNF-α and NO."/>
新生大鼠内毒素休克时血清TNF-α和NO对心肌超微结构的影响(英文)
Effects of Serum TNF-α and NO on Morphology of Myocardial Tissues in Neonatal Rats with Endotoxic Shock
WANG Hong, WU Yu-Bin, DU Xiu-Hua, PAN Jing-Kun
Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shengyang 110004, China; 2. Department of Nursing, Shenyang Medical College, Shenyang 110015, China
Abstract：OBJECTIVE: To study the changes of serum TNF-α and NO in neonatal rats with endotoxic shock and its relationship with myocardial cells damge and to explore the protection effect of dexamethasone (DXM) on neonatal rats with endotoxic shock. METHODS: Nine of the 117 seven-day-old healthy neonatal Wistar rats were used as the pre-experimental base value control group, and the other 108 rats were randomly divided into control group, endotoxic shock group (LPSgroup, LPS 5 nig/kg) and treatment group (DXM group, LPS 5 mg/kg + DXM 5 mg/kg). Before and after injection of LPS (2, 4, 6 and 24 hs), 9 rats in each group were sacrificed and blood samples were collected to detect TNF-a by ELISA and NO by nitrate reductase. Myocardial super-microstructure was observed under an electron microscope. RESULTS: ① In the LPS group, the concentration of serum TNF-α peaked at 2 h, and it decreased to the level of control group after 6 hs. In the DXM group, the level of TNF-α at 2 h was higher than that of control group ( P < 0.05), but lower than that of the LPS group ( P < 0.05). At 4 hs the level TNF-α in DXM group was lower than that of the LPS group ( P <0.05), but was not different than that of the control group ( P >0.05). From 6 h after injection, the differences of TNF-α levels in 3 groups were not obvious ( P >0.05). ② In the LPS group, the concentration of NO rose after 2 h ( P <0. 01), and peaked at 24 h ( P <0. 01). After 2 hs, the levels of NO in the DXM group were lower than those of the LPS group ( P < 0.01). ③ Six hours after the injection of LPS, a little mitochondria of myocardial cells in the LPS group appeared to develop vacuolae-like degeneration. At 24 h, most mitochondria of myocardial cells in the LPS group presententecl with vaculea-like degeneration and necrosis. The myocardial fibers were broken. While in the DXM group, the changes in the super-microstructure at 24 h were not as serious as those which talk place in the LPS group. CONCLUSIONS: TNF-α and No were involved in the damage of myocardial cells in neonatal rats with endotoxic shock. DXM could partly protect the myocardial cells from damage by inhibiting the production of TNF-α and NO.
WANG Hong,WU Yu-Bin,DU Xiu-Hua et al. Effects of Serum TNF-α and NO on Morphology of Myocardial Tissues in Neonatal Rats with Endotoxic Shock[J]. CJCP, 2003, 5(5): 403-406.
