OBJECTIVE: To study the relationship between the change of interleukin 5 (IL 5) level in induced sputum and degrees of asthma attack in children with acute asthma, and to study the role of IL 5 in pathogenesis of asthma. METHODS: Sixty five cases of acute asthma were divided into 3 groups according to the degrees of asthma attack: mild, moderate and severe groups. Thirty four healthy children were served as the control group. Ultrasonically nebulized hypertonic saline was used to induce sputum. The IL 5 level in induced sputum was determined using ELISA. The forced expiratory ratio (FEV 1) and the eosinephils (EOS) counting in the sputum were measured. RESULTS: The EOS counting and IL 5 level in the induced sputum of children with acute asthma attack increased compared with those in the control group, whereas the FEV 1 decreased in the former (P<0.01 ). As asthma state became worse, EOS counting in induced sputum increased, but no significant difference was noted among different degrees of athma attack. The IL 5 level in the induced sputum was obviously different among the mild, moderate and severe cases [( 8.8 ± 4.9 ), ( 82.7 ± 173.5 ) and ( 225.1 ± 228.9 ) pg/ml, respectively] (P< 0.05 ). The FEV 1 in the severe group was significantly lower than those in the mild and moderate groups (P< 0.05 ). The IL 5 level in induced sputum was significantly positively correlated with EOS counting (r= 0.482 ,P< 0.05 ) and significantly negatively correlated with FEV 1 (r= -0.647 ,P< 0.01 ). CONCLUSIONS: Compared with EOS counting, the IL 5 level in induced sputum can more accurately reflect the degrees of asthma attack, indicating that it may be used as a sensitive index for evaluating the asthmatic state of children and the therepeutic effectiveness of drugs."/>
急性发作期哮喘患儿诱导痰中白细胞介素-5水平变化及其临床意义
Abstract：OBJECTIVE: To study the relationship between the change of interleukin 5 (IL 5) level in induced sputum and degrees of asthma attack in children with acute asthma, and to study the role of IL 5 in pathogenesis of asthma. METHODS: Sixty five cases of acute asthma were divided into 3 groups according to the degrees of asthma attack: mild, moderate and severe groups. Thirty four healthy children were served as the control group. Ultrasonically nebulized hypertonic saline was used to induce sputum. The IL 5 level in induced sputum was determined using ELISA. The forced expiratory ratio (FEV 1) and the eosinephils (EOS) counting in the sputum were measured. RESULTS: The EOS counting and IL 5 level in the induced sputum of children with acute asthma attack increased compared with those in the control group, whereas the FEV 1 decreased in the former (P<0.01 ). As asthma state became worse, EOS counting in induced sputum increased, but no significant difference was noted among different degrees of athma attack. The IL 5 level in the induced sputum was obviously different among the mild, moderate and severe cases [( 8.8 ± 4.9 ), ( 82.7 ± 173.5 ) and ( 225.1 ± 228.9 ) pg/ml, respectively] (P< 0.05 ). The FEV 1 in the severe group was significantly lower than those in the mild and moderate groups (P< 0.05 ). The IL 5 level in induced sputum was significantly positively correlated with EOS counting (r= 0.482 ,P< 0.05 ) and significantly negatively correlated with FEV 1 (r= -0.647 ,P< 0.01 ). CONCLUSIONS: Compared with EOS counting, the IL 5 level in induced sputum can more accurately reflect the degrees of asthma attack, indicating that it may be used as a sensitive index for evaluating the asthmatic state of children and the therepeutic effectiveness of drugs.
LU Zheng-Rong,WANG Xiao-Lei,WANG Ci-Lin et al. Change of the Interleukin-5 Level in Induced Sputum of Children with Acute[J]. CJCP, 2003, 5(4): 322-324.
