OBJECTIVE: To study the protective effect of estradiol on immature myocardium of rabbits with ischemia reperfusion injury. METHODS: The isolated perfused heart model was developed in thirty two 3 to 4 week old female Japanese rabbits. The rabbits were randomly assigned into four groups according to the perfusion solutions for cardiac arrest (n=8): the control group, estradiol group, blocker group and estradiol+blocker group. In the control group, only St Thomasll solution was used; 10 -6 mol/L of 17 β estradiol was added in the St ThomasII solution in the estradiol group; the blocker group was perfused the St Thomasll solution containing 10 -6 mol/L of 4 Hydroxy Tamoxifen (the blocker of estradiol); and the last group, both 10 -6 mol/L of estradiol and 4 Hydroxy Tamoxifen were added in St Thomas II solution. Sixty minutes after cardiac arrested, reperfusion began. The coronary flow (CF), left ventricular developed pressure (LVDP), ±dp/dt max , mycocardial water content, adenosine triphosphate (ATP) and malondialdehyde (MDA) contents of myocardium, creatine phosphokinase (CK) and lactic dehydrogenase (LDH) and glutamic oxalacetic transaminase (GOT) leakage of coronary effluent and the pathologic changes of ultrastructure of myocardium in the four groups were measured at 10, 20, 40, 60 and 90 min after reperfusion. RESULTS: The recovery rate of CF and the recovery rate of LVDP and ±dp/dt max in the estradiol group from 60 min and 40 min respectively after reperfusion were higher than those of the other three groups (P< 0.01 or 0.05 ). The contents of water, MDA, and CK, LDH, GOT leakage of coronary effluent in myocardium of the estradiol group were lower compared with those of the other three groups. The ATP content of myocardium in the estradiol group was higher than that of the control group and blocker group. The ultrastructural pathologic changes of myocardium in the estradiol group were slightest in the four groups. CONCLUSIONS: Estradiol has the protective effect on the immature myocardium of rabbits and the mediator is the estrogen receptor."/>
雌二醇对幼兔未成熟心肌缺血-再灌注损伤的保护作用
Abstract：OBJECTIVE: To study the protective effect of estradiol on immature myocardium of rabbits with ischemia reperfusion injury. METHODS: The isolated perfused heart model was developed in thirty two 3 to 4 week old female Japanese rabbits. The rabbits were randomly assigned into four groups according to the perfusion solutions for cardiac arrest (n=8): the control group, estradiol group, blocker group and estradiol+blocker group. In the control group, only St Thomasll solution was used; 10 -6 mol/L of 17 β estradiol was added in the St ThomasII solution in the estradiol group; the blocker group was perfused the St Thomasll solution containing 10 -6 mol/L of 4 Hydroxy Tamoxifen (the blocker of estradiol); and the last group, both 10 -6 mol/L of estradiol and 4 Hydroxy Tamoxifen were added in St Thomas II solution. Sixty minutes after cardiac arrested, reperfusion began. The coronary flow (CF), left ventricular developed pressure (LVDP), ±dp/dt max , mycocardial water content, adenosine triphosphate (ATP) and malondialdehyde (MDA) contents of myocardium, creatine phosphokinase (CK) and lactic dehydrogenase (LDH) and glutamic oxalacetic transaminase (GOT) leakage of coronary effluent and the pathologic changes of ultrastructure of myocardium in the four groups were measured at 10, 20, 40, 60 and 90 min after reperfusion. RESULTS: The recovery rate of CF and the recovery rate of LVDP and ±dp/dt max in the estradiol group from 60 min and 40 min respectively after reperfusion were higher than those of the other three groups (P< 0.01 or 0.05 ). The contents of water, MDA, and CK, LDH, GOT leakage of coronary effluent in myocardium of the estradiol group were lower compared with those of the other three groups. The ATP content of myocardium in the estradiol group was higher than that of the control group and blocker group. The ultrastructural pathologic changes of myocardium in the estradiol group were slightest in the four groups. CONCLUSIONS: Estradiol has the protective effect on the immature myocardium of rabbits and the mediator is the estrogen receptor.
TAN Jiang-Ning,LONG Cun,HAN Ling. Protective Effect of Estradiol on lmmature Myocardium of Rabbits with IschemiaReperfusion Injury[J]. CJCP, 2003, 5(3): 205-0.
