OBJECTIVE: To study the dose-effect and time-effect of heat shock protein-70 (HSP70) expression induced by curcumin, an antioxidant compound extracted from the spice tumeric, and the protective effect of curcumin on infectious brain edema in rats. METHODS: Dose-effect and time-effect: 24 SD rats were randomly assigned into four groups: control group, dimethyl sulfaxide (DMSO) group, heat shock group and curcumin group. The rats in the curcumin group were subdivided into groups receiving 80, 40, 20 and 10 mg of curcumin, respectively. Another 24 SD rats injected with 40 mg curcumin were divided into groups sacrificed at 0 h, 2 h, 4 h, 6 h, 12 h, 16 h, 24 h, and 48 h. HSP70 expression was detected by the Western blotting analysis. Protective effect of curcumin on infectious brain edema: 52 SD rats were assigned into five groups: normal control group, infectious brain edema group, DMSO pretreatmentgroup (DMSO group), heat shock pretreatment group (HS group), and curcumin pretreatment group (CUR group). Water content and Na+ and K+ contents in brain tissues were measured. RESULTS: Compared with the control group, HSP70 expressions of the heat shock group, 40 mg curcumin group and 80 mg curcumin group significantly increased, especially in the 40 mg group ( P <0.01). HSP70 expression gradually increased with the time after the injection of curcumin, peaking at 16 h and reaching a plateau at 24 h and 48 h. The contents of water and Na+ in brain tissues significantly decreased in the HS and CUR groups compared with the un-treated infectious brain edema group. CONCLUSIONS: HSP70 expression can be induced by pretreatment with curcumin, and there are dose-effects and time-effects. The protective effect of curcumin against infectious brain edema may be a consequence of increased HSP70 expression in rats."/>
姜黄素诱导HSP70及对大鼠感染性脑水肿保护作用的研究
Abstract：OBJECTIVE: To study the dose-effect and time-effect of heat shock protein-70 (HSP70) expression induced by curcumin, an antioxidant compound extracted from the spice tumeric, and the protective effect of curcumin on infectious brain edema in rats. METHODS: Dose-effect and time-effect: 24 SD rats were randomly assigned into four groups: control group, dimethyl sulfaxide (DMSO) group, heat shock group and curcumin group. The rats in the curcumin group were subdivided into groups receiving 80, 40, 20 and 10 mg of curcumin, respectively. Another 24 SD rats injected with 40 mg curcumin were divided into groups sacrificed at 0 h, 2 h, 4 h, 6 h, 12 h, 16 h, 24 h, and 48 h. HSP70 expression was detected by the Western blotting analysis. Protective effect of curcumin on infectious brain edema: 52 SD rats were assigned into five groups: normal control group, infectious brain edema group, DMSO pretreatmentgroup (DMSO group), heat shock pretreatment group (HS group), and curcumin pretreatment group (CUR group). Water content and Na+ and K+ contents in brain tissues were measured. RESULTS: Compared with the control group, HSP70 expressions of the heat shock group, 40 mg curcumin group and 80 mg curcumin group significantly increased, especially in the 40 mg group ( P <0.01). HSP70 expression gradually increased with the time after the injection of curcumin, peaking at 16 h and reaching a plateau at 24 h and 48 h. The contents of water and Na+ in brain tissues significantly decreased in the HS and CUR groups compared with the un-treated infectious brain edema group. CONCLUSIONS: HSP70 expression can be induced by pretreatment with curcumin, and there are dose-effects and time-effects. The protective effect of curcumin against infectious brain edema may be a consequence of increased HSP70 expression in rats.
HUANG Rong,LUO Fang,YU Xiao-He et al. Heat shock protein-70 expression induced by curcumin and the protective effect of curcumin on infections brain edema in rats[J]. CJCP, 2003, 5(2): 109-112.
