目的：了解兰州地区回族与汉族儿童维生素D受体(VDR)基因多态性分布,探讨不同种族之间VDR基因型频率的分布。方法：利用限制性内切酶Bsm Ⅰ,采用聚合酶链反应限制性片段长度多态性技术(PCR-RFLP),对兰州地区回族健康儿童81例和汉族健康儿童169例VDR基因多态性进行检测。结果：发现在回族81例中,bb型57例(70.4％),Bb型22例(27.2％),BB型2例(2.5％);在汉族169例中,bb型154例(91.1％),Bb型12例(7.1％),BB型3例(1.8％)。结论：兰州地区回族VDR基因多态性分布与汉族相比存在显著性差异。

OBJECTIVE: To investigate the distribution of vitamin D receptor (VDR) gene polymorphism in Hui and Han ethnic children in Lanzhou region and to study the difference between children of the two different ethnic groups. METHODS: VDR genotypes were assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 81 healthy Hui ethnic children and in 169 healthy Han ethnic children. RESULTS: Frequencies of bb, Bb and BB genotype were 70. 4% , 27. 2% and 2.5% respectively in the 81 Hui ethnic children and they were 91.1 % , 7.1% and 1.8% respectively in the 169 Han ethnic children. CONCLUSIONS: There is a significant difference in the frequency distribution of VDR genotype between Hui and Han ethnic children. [Chin J Contemp Pediatr, 2003, 5(1): 37-38]