OBJECTIVE: To study the significance of the micro blood sample for surface antibody of red blood cells (RBC) in the diagnosis of childhood autoimmune hemolytic anemia (AIHA). METHODS: The RBC surface antibody was measured in 145 children with suspected AIHA using the micro blood sample. Of them, 30 cases, besides using the micro blood sample, were measured using the routine method -- the direct Coombs test by taking venous blood of defibrinogen at the same time. RESULTS: Of the 145 patients 13 were diagnosed AIHA by the micro blood sample. The direct Coombs test was positive in the 13 cases. Seven of them had a positive result with univalent antibody IgG, 1 with IgG+C3, 3 with IgG+IgM+C3 and 2 with IgM+C3. In 30 cases with the two tests for the RBC surface antibody, 4 had a positive result with the direct Coombs test. The results of the two methods were consistent. In 26 negative cases, there was a false positive ( 3.85% ) of univalent antibody IgM by the minim finger blood test, and 2 false positive ( 7.69% ) of IgM using the routine method. There was no statistic difference between the two methods (P> 0.05 ). No difference was noted in the protein of supernatant fluid after centrifugation between the two methods (P> 0.05 ). CONCLUSIONS: The micro blood sample for RBC surface antibody in the diagnosis of childhood AIHA is reliable and convenient for re test."/>
微量血检测红细胞表面抗体诊断小儿自身免疫性溶血性贫血研究
Abstract：OBJECTIVE: To study the significance of the micro blood sample for surface antibody of red blood cells (RBC) in the diagnosis of childhood autoimmune hemolytic anemia (AIHA). METHODS: The RBC surface antibody was measured in 145 children with suspected AIHA using the micro blood sample. Of them, 30 cases, besides using the micro blood sample, were measured using the routine method -- the direct Coombs test by taking venous blood of defibrinogen at the same time. RESULTS: Of the 145 patients 13 were diagnosed AIHA by the micro blood sample. The direct Coombs test was positive in the 13 cases. Seven of them had a positive result with univalent antibody IgG, 1 with IgG+C3, 3 with IgG+IgM+C3 and 2 with IgM+C3. In 30 cases with the two tests for the RBC surface antibody, 4 had a positive result with the direct Coombs test. The results of the two methods were consistent. In 26 negative cases, there was a false positive ( 3.85% ) of univalent antibody IgM by the minim finger blood test, and 2 false positive ( 7.69% ) of IgM using the routine method. There was no statistic difference between the two methods (P> 0.05 ). No difference was noted in the protein of supernatant fluid after centrifugation between the two methods (P> 0.05 ). CONCLUSIONS: The micro blood sample for RBC surface antibody in the diagnosis of childhood AIHA is reliable and convenient for re test.
LI Yun,YI Hong-Ling,HE Xiang-Ling et al. Micro Blood Sample for RBC Surface Antibody in the Diagnosis of Childhood Autoimmune Hemolytic Anemia[J]. CJCP, 2002, 4(1): 15-17.
