Abstract:OBJECTIVE: To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-γ and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP. METHODS: Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-γ and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls. RESULTS: Blood T-bet mRNA expression and IFN-γ levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (P<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (P<0.01). CONCLUSIONS: The high expression of T-bet and IFN-γ and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.[Chin J Contemp Pediatr, 2010, 12 (1):29-31]
REN Shao-Min,ZHANG Chun-Xia,LI Wei-Cai. Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura[J]. CJCP, 2010, 12(1): 29-31.
[6]Lametschwandtner G, Biedermann T, Schw-rzler C, Günther C, Kund J, Fassl S, et al. Sustained T-bet expression confers polarized human TH2 cells with TH1-like cytokine production and migratory capacities[J]. J Allergy Clin Immunol, 2004, 113(5): 987-994.
[7]Su RC, Becker AB, Kozyrskyj AL, Hayglass KT.Epigenetic regulation of established human type 1 versus type 2 cytokine responses[J]. J Allergy Clin Immunol, 2008,121(1):57-63.
[8]Inman D, Kawana K, Schust D, Lininger R, Young S. Cyclic regulation of T-Bet and GATA-3 in human endometrium[J]. Reprod Sci, 2008,15(1):83-90.
[11]Chakir H, Wang H, Lefebvre DE, Webb J, Scott FW.T-bet/GATA3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3[J]. J Immunol Meth, 2003, 278(1-2):157-169.