Abstract：OBJECTIVE: To study the value of eosinophils (EOS) and interleukin-17 (IL-17) in nasopharyngeal secretions in the evaluation of progress of wheezing in children under 5 years old. METHODS: Fifty-three children under five years old who had recurrent wheezing were classified into two groups: wheezing group I with atopic body (n=27) and wheezing group II without atopic body (n=26). Twenty pre-surgical children with non-infectious disease were used as the control group. Nasopharyngeal secretions were collected. Inflammatory cells in nasopharyngeal secretions were counted under the microscope. IL-17 levels in supernatants were measured using ELISA. RESULTS: EOS counts in nasopharyngeal secretions in wheezing group I were significantly higher than those in wheezing group II and the control group (P<0.05, P<0.01, respectively). There were no significant differences in EOS counts between wheezing II and the control groups. The IL-17 levels in both wheezing groups were significantly higher than those in the control group (P<0.01), and the wheezing group I had increased IL-17 levels than wheezing group II (1 474±974 pg/mL vs 788±132 pg/mL; P<0.05). The IL-17 level was positively correlated with the EOS counts in wheezing group I (r=0.62, P<0.05). CONCLUSIONS: EOS counts and IL-17 levels in nasopharyngeal secretions may be used as indices for identifying the tendency to develop asthma in children under 5 years old with wheezing.［Chin J Contemp Pediatr, 2010, 12 (2):113-116］
WANG Xiu-Fang,YANG Jin-Ling,QIAO Jun-Ying et al. Measurement of eosinophils and interleukin-17 in nasopharyngeal secretions of children under 5 years old with wheezing[J]. CJCP, 2010, 12(2): 113-116.
［2］Amado MC, Portnoy JM. Diagnosing in asthma in young children［J］. Curr Opin Allergy Clin Immunol, 2006, 6(2):101-105.
［3］Moore WC, Peters SP. Severe asthma: an overview［J］.J Allergy Clin Immunol, 2006, 117(3):487-494.
［4］Gibson PG, Henry RL, Thomas P. Noninvasive assessment of airway inflammation in children: induced sputun, exhaled nitric oxide, and breath condensate［J］. Eur Respir J, 2000, 16(5)：1008-1015．
［5］Wilson NM, Bridge P, Spanevello A, Silverman M. Induced sputun in children: feasibility, repeatability and relation of findings to asthma severity［J］. Thorax, 2000, 55(9): 768-774.
［7］Henness S, van Thoor E, Ge Q, Armour CL, Hughes JM, Ammit AJ. IL-17A act via p38 MAPK to increase stability of TNF-alpa-induced Il-8 mRNA in human ASM［J］.Am J Physiol Lung cell Mol Physiol, 2006, 290(6):L1283-1290.
［8］Murphy KR, Berger WE. The variability of asthma［J］.Curr Med Res Opin, 2005, 21(10):1519-1526.
［9］Nagayama Y, Tsubaki T, Toba T, Nakayama S, Kiyofumi O. Analysis of sputun taken from wheezy and asthmatic infants and children, with special reference to respiratory infections［J］．Pediatr Allergy Immunol, 2001, 12(6):318-326.
［10］Castro-Rodriguez JA, Ramirez AM, Toche P, Pavon D, Perez MA, Girardi G, et al. Clinical, functional, and epidemiological differences between atopic and nonatopic asthmatic children from a tertiary care hospital in a developing country［J］. Ann Allergy Asthma Immunol, 2007, 98(3):239-244.
［11］Molet S, Hamid Q, Davoine F, Nutku E, Taha R, Page N, et al. IL-17 is increased in asthmatic airways and induces human bronchial fibroblasts to produce cytokines［J］.J Allergy Clin Immunol, 2001, 108(3):430-438.
［12］Yasui K, Kobayashi N, Yamazaki T, Koike K, Fukushima K, Taniuchi S, et al. Neutrophilis inflammation in childhood bronchial asthma［J］. Thorax, 2005, 60(8):704-707.
［13］Jarjour NN, Gern JE, Kelly EA, Swenson CA, Dick CR, Busse WW. The effect of an experimental rhinovirus 16 infection on bronchial lavage neutrophils［J］. J Allergy Clin Immunol, 2000, 105(6 Pt 1):1169-1177.
