cAMP反应元件结合蛋白/Bcl-2在小儿急性白血病骨髓细胞中的表达及意义

文川; 马夫天; 万伍卿

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中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (3) : 177-180.

论著·临床研究

cAMP反应元件结合蛋白/Bcl-2在小儿急性白血病骨髓细胞中的表达及意义

文川，马夫天，万伍卿

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Expression of CREB/Bcl-2 in bone marrow mononuclear cells of children with acute leukemia

WEN Chuan, MA Fu-Tian, WAN Wu-Qing

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摘要

目的：探讨cAMP反应元件结合蛋白(CREB)和Bcl-2在小儿急性白血病骨髓细胞中的表达及临床意义。方法：92例急性白血病患儿和30例非恶性血液病患儿（对照组）纳入研究。采用RT-PCR和Western blot检测CREB和Bcl-2在骨髓单个核细胞内的表达。结果：CREB和Bcl-2在急性白血病患儿骨髓中的表达明显高于对照组(P＜0.01)；CREB和Bcl-2在急性淋巴细胞白血病（ALL）和急性髓细胞白血病（AML）患儿骨髓中表达差异无统计学意义；髓外浸润组患儿骨髓CREB和Bcl-2的表达明显高于非髓外浸润组患儿(P＜0.05)；完全缓解组CREB和Bcl-2的表达显著低于非完全缓解组(P＜0.01)；急性白血病组患儿骨髓CREB和Bcl-2 mRNA的表达与外周血白细胞计数呈正相关，相关系数分别为0.62和0.71 (P＜0.05)；CREB和Bcl-2 mRNA 和蛋白表达分别呈正相关，相关系数分别为0.75和0.68(P＜0.05)。结论：CREB和Bcl-2表达可能与小儿白血病发病机制和临床预后存在相关性，但可能与小儿急性白血病类型无相关性。［中国当代儿科杂志，2010，12（3）：177-180］

Abstract

ObjectiveTo study the expression and role of cyclic-AMP response binding protein (CREB) and Bcl-2 in children with acute leukemia. METHODS: Ninety-two children with acute leukemia (leukemia group) and 30 children with non-hematologic malignancies (control group) were enrolled. The mRNA and protein expression of CREB and Bcl-2 in bone marrow mononuclear cells were measured by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot. RESULTS: The mRNA and protein expression of CREB and Bcl-2 in the leukemia group was significantly higher than that in the control group (P<0.01). There were no significant differences in the expression of CREB and Bcl-2 between acute lymphoblastic leukemia and acute myeloid leukemia subgroups. At the initial diagnosis, the mRNA and protein expression of CREB and Bcl-2 in children with extramedullary infiltration was higher than that in children without (P<0.05). In the leukemia group, the mRNA and protein expression of CREB and Bcl-2 in the complete remission subgroup was significantly lower than that in the non-complete remission subgroup (P<0.01). High mRNA expression of CREB and Bcl-2 in the leukemia group was positively correlated with peripheral blood leucocyte counts (r=0.62, 0.71 respectively, P<0.05). There was a positive correlation between mRNA and protein expression of CREB and Bcl-2 (r=0.75, 0.68 respectively; P<0.05). CONCLUSIONS: The expression of CREB and Bcl-2 may be correlated with the pathogenesis and clinical prognosis of childhood leukemia, however, their expression may not be associated with the classification of acute leukemia.［Chin J Contemp Pediatr, 2010, 12 (3):177-180］

导出引用

文川, 马夫天, 万伍卿. cAMP反应元件结合蛋白/Bcl-2在小儿急性白血病骨髓细胞中的表达及意义[J]. 中国当代儿科杂志. 2010, 12(3): 177-180

WEN Chuan, MA Fu-Tian, WAN Wu-Qing. Expression of CREB/Bcl-2 in bone marrow mononuclear cells of children with acute leukemia[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(3): 177-180

中图分类号： R733.71

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