上海地区部分儿童幽门螺杆菌cagA、vacA、iceA基因型别分析

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摘要目的：了解上海部分地区儿童感染幽门螺杆菌（Hp）的cagA、vacA、iceA的基因亚型，探讨其与儿童上消化道疾病的关系。方法：收集2007年5月至2008年1月在我院行胃镜检查确诊Hp感染59例患者的胃黏膜组织，分别进行聚合酶链反应（PCR）检测cagA、vacA和iceA基因；病理检查胃窦黏膜炎症程度；酶联免疫吸附试验（ELISA）检测胃窦黏膜IFN-γ 和IL-4的含量。结果：cagA基因单独检出率为65%（37/57），vacAs1/m1单独检出率为19%（11/57）,vacAs1/m2单独检出率为40%（23/57），iceA1单独检出率为63%（36/57），iceA2单独检出率为19%（11/57），9%（5/57）的菌株iceA1 和iceA2均阳性。不同基因型菌株在慢性胃炎和消化性溃疡中的检出率差异无统计学意义（P>0.05）。不同基因型菌株与胃窦黏膜炎症的严重程度无关（P>0.05）。不同基因型菌株感染的胃窦黏膜IFN-γ、IL-4的含量差异亦无统计学意义（P>0.05）。结论：cagA/vacAs1/m2/iceA1为上海部分地区儿童中Hp的优势基因型。除了菌株因素外，宿主基因多态性、环境因素对于疾病的发生、发展也发挥了重要作用。［中国当代儿科杂志，2010，12（4）：267-271］

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关键词 ：幽门螺杆菌, cagA, vacA, iceA, 儿童

Abstract：OBJECTIVE: To investigate cagA, vacA and iceA genotypes of Helicobacter pylori (H. pylori) isolated from children suffering from gastric and duodenal diseases in Shanghai and to explore a possible genotype-phenotype correlation. METHODS: From May 2007 to January 2008, 59 children were confirmed with Hp infection by gastroscopy. Biopsied specimens were taken from the gastric antrum. cagA, vacA and iceA genes were determined by PCR. The histological changes in the gastric mucosa were evaluated. The levels of IFN-γ and IL-4 in the gastric mucosa were measured using ELISA. RESULTS: cagA, vacAs1/m1, vacAs1/m2, iceA1 and iceA2 were found in 65%, 19%, 40%, 63% and 19% of H. pylori strains, respectively. Both iceA1 and iceA2 were detected in 9% of strains. There were no statistical differences in the distribution of various genotypes between the children with chronic gastritis and peptic ulcer. No association was observed between the genotypes and the degree of inflammation of gastric mucosa. There were no significant differences in levels of IFN-γ and IL-4 in the gastric mucosa infected by different genotypes of H. pylori strains. CONCLUSIONS: cagA/vacAs1/m2/iceA1 may be the commonest genotype combination of H.pylori in children from Shanghai. That there was no association between H.pylori genotypes and clinical variables suggests the potential role of host and environment factors in the development of clinical diseases at a later life.［Chin J Contemp Pediatr, 2010, 12 (4):267-271］

Key words： Helicobacter pylori cagA vacA iceA Child

PACS:

R725.7

引用本文:

周颖,黄瑛,邵彩虹等. 上海地区部分儿童幽门螺杆菌cagA、vacA、iceA基因型别分析[J]. 中国当代儿科杂志, 2010, 12(04): 267-271.

ZHOU Ying,HUANG Ying,SHAO Cai-Hong et al. cagA, vacA and iceA genotypes of Helicobacter pylori isolated from children in Shanghai[J]. CJCP, 2010, 12(04): 267-271.

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http://www.zgddek.com/CN/ 或 http://www.zgddek.com/CN/Y2010/V12/I04/267

	［1］Go MF. Review article: natural history and epidemiology of Helicobacter pylori infection［J］. Aliment Pharmacol Ther, 2002, 16(Suppl 1):3-15.
	［2］Correa P. Newstrategies for the prevention of gastric cancer: Helicobacter pylori and genetic susceptibility ［J］. J Surg Oncol, 2005, 90(3):134-138.
	［3］Figueiredo C, Van Doorn LJ, Nogueira C, Soares JM, Figueira P, Quint WG, et al. Helicobacter pylori genotypes are associated with clinical outcome in Portuguese patients and reveal a high prevalence of infections with multiple strains［J］. Scand J Gastroenterol, 2001, 36(2):128-135.
	［4］Oliviera AM, Queiroz DM, Rocha GA, Mendes EN. Seroprevalence of Helicobacter pylori in children of low socioeconomic level in Belo Horizonte, Brazil［J］. Am J Gastroenterol, 1994, 89(12):2201-2204.
	［5］Zambon CF, Basso D, Navaglia F, Mazza S, Razetti M, Fogar P, et al. Non-invasive diagnosis of Helicobacter pylori infection: simplified 13Curea breath test, stool antigen testing or DNA PCR in human faeces in a clinical laboratory setting［J］. Clin Biochem, 2004, 37(4): 261-267.
	［6］中华医学会消化病学分会.中国慢性胃炎共识意见(上)(2006,上海)［J］.中华消化杂志, 2007, 27(1):49-50.
	［7］Van Doorn LJ, Figueiredo C, Rossau R, Jannes G, van Asbroek M, Sousa JC, et al. Typing of Helicobacter pylori vacA gene by PCR and reverse hybridization［J］. J Clin Microbiol, 1998, 36(5):1271-1276.
	［8］Yamaoka Y, Kodama T, Gutierrez O, Kim JG, Kashima K, Graham DY. Relationship between Helicobacter pylori iceA cagA and vacA status and clinical outcome: studies in four different countries［J］. J Clin Microbiol, 1999, 37(7):2274-2279.
	［9］Peek RM Jr, Thompson SA, Donohue JP, Tham KT, Atherton JC, Blaser MJ, et al. Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome［J］. Proc Assoc Am Phys, 1998, 110(6):531-544.
	［10］Elviss NC, Owen RJ, Xerry J, Walker AM, Davies K. Helicobacter pylori antibiotic resistance patterns and genotypes in adult dyspeptic patients from a regional population in North Wales［J］. J Antimicrob Chemother, 2004, 54(2):435-440.
	［11］Russo F, Berloco P, Cuomo R, Caruso ML, Di Matteo G, Giorgio P, et al. Helicobacter pylori strains and histologically-related lesions affect the outcome of triple eradication therapy:a study from southern Italy［J］. Aliment Pharmacol Ther, 2003, 17(3):421-428.
	［12］Ito Y, Azuma T, Ito S, Miyaji H, Hirai M, Yamazaki Y, et al. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan［J］.J Clin Microbiol, 1997, 35(7):1710-1714.
	［13］杜奕奇，许国铭，纪徐淮，丁华，张洪富，满晓华，等.细胞毒素相关抗原（cagA）基因在中国人幽门螺杆菌中的分布及其临床意义［J］.中华消化杂志， 1999，19（3）：165-167.
	［14］许春娣, 郑洁, 奚容平, 陈舜年，徐家裕. 具有cagA、vacA基因的幽门螺杆菌感染及其与胃十二指肠疾病的关系［J］. 中华儿科杂志, 2002, 40(3):144-147.
	［15］林燕芬, 龚四堂, 区文玑, 潘瑞芳, 黄海, 何婉儿, 等. 广州地区患儿感染幽门螺杆菌vacA、cagA及iceA基因研究［J］. 中华儿科杂志, 2007, 45(9):703-707.
	［16］Benenson S, Halle D, Rudensky B, Faber J, Schlesinger Y, Branski D, et al. Helicobacter pylori genotypes in Israeli children:the significance of geography［J］. J Pediatr Gastroenterol Nutr, 2002, 35(5):680-684.
	［17］Perng CL, Lin HJ, Sun IC, Tseng GY, Facg. Helicobacter pylori cagA, iceA and vacA status in Taiwanese patients with peptic ulcer and gastritis［J］. J Gastroenterol Hepatol, 2003, 18(11):1244-1249.
	［18］陈晶, 方平楚, 陶然, 尤建飞, 茅海燕. 浙江地区幽门螺杆菌优势基因型研究［J］.浙江预防医学, 2006, 18(3):1-3.
	［19］Costa Lopes AI, Palha A, Monteriro L, Olcastro M, Pelerito A, Fernandes A. Helicobacter pylori genotypes in children from a population at high gastric cancer risk: no association with gastroduodenal histopathology［J］. Am J Gastroenterol, 2006, 101(9):2113-2122.
	［20］Leanza AG, Matteo MJ, Crespo O, Antelo P, Olmos J, Catalano M, et al. Genetic characterization of Helicobacter pylori isolates from an Argentinean adult population based on cag pathogenicity island right-end motifs, lspA-glmM polymorphism and iceA and vacA genotypes［J］. Clin Microbiol Infect, 2004, 10(9):811-819.
	［21］Ko JS, Kim KM, Oh YL, Seo JK. cagA, vacA, and iceA genotypes of Helicobacter pylori in Korean children［J］. Pediatr Int, 2008, 50(5):628-631.
	［22］林燕芬, 龚四堂, 区文玑, 潘瑞芳, 王凤华, 周荣, 等. 广州地区儿童感染幽门螺杆菌iceA基因亚型与胃炎的相关性研究［J］. 广东医学, 2007, 28(2):213-215.
	［23］Dzierzanowska-Fangrat K, Crabtree JE, Rozynek E, Dura W, Celinska-Cedro D, Wojda U, et al. Helicobacter pylori cagA genotype and density of colonization in relation to gastric inflammation in children［J］. Eur J Gastroenterol Hepatol, 2002, 14(12):1303-1307.
	［24］Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of helicobacter pylori infection［J］. Clin Microbiol Rev, 2006, 19(3):449-490.
	［25］Shimizu T, Haruna H, Ohtsuka Y, Kaneko K, Gupta R, Yamashiro Y. Cytokines in the gastric mucosa of children with Helicobacter pylori infection［J］. Acta Paediatr, 2004, 93(3):322-326.
	［26］Bontems P, Robert F, Van Gossum A, Cadranel S, Mascart F. Helicobacter pylori of gastric and duodenal mucosal T cell cytokine secretions in children compared with adults［J］. Helicobacter, 2003, 8(3):216-226.
	［27］Straubinger RK, Greiter A, Mcdonough SP, Gerold A, Scanziani E, Soldati S, et al. Quantitative evaluation of inflammatory and immune responses in the early stages of chronic Helicobacter pylori infection［J］. Infect Immun, 2003, 71(5):2693-2703.
	［28］Orsini B, Ottanelli B, Amedei A, Surrenti E, Capanni M, Del Prete G, et al. Helicobacter pylori cag pathogenicity island is associated with reduced expression of interleukin-4(IL-4)mRNA and modulation of the IL-4 delta2 mRNA isoform in human gastric mucosa［J］. Infect Immun, 2003, 71(11): 6664-6667.