Abstract：OBJECTIVE: To investigate the significance of soluble DLL1 (Delta-like-1) levels of cerebrospinal fluid (CSF) and serum in the diagnosis of intracranial infection in children. METHODS: Fifty children with intracranial infection, including 20 cases of tuberculous meningitis (TM), 20 cases of viral meningitis (VM) and 10 cases of purulent meningitis (PM), and 20 children without intracranial infection (control group) were enrolled. The levels of soluble DLL1 in CSF and serum were measured using ELISA. RESULTS: The level of CSF soluble DLL1 in the TM group was significantly higher than that in the VM, PM and control groups (2.89±1.72 ng/mL vs 0.14±0.14 ng/mL, 0.27±0.21 ng/mL, 0.13±0.12 ng/mL; P<0.01). The level of serum soluble DLL1 in the TM group was also significantly higher than that in the VM, PM and control groups (12.61±6.45 ng/mL vs 2.28±2.27 ng/mL, 2.38±1.79 ng/mL, 2.26±2.10 ng/mL; P<0.01). The levels of soluble DLL1 in the CSF and serum in the VM and PM groups were not significantly different from those in the control group. CONCLUSIONS: Soluble DLL1 as a novel indicator might have potentially important value in the diagnosis of TM.
LI Jin-Yi,JIA Yan-Jie,JIA Yong-Lin et al. Significance of soluble DLL1 in diagnosis of intracranial infectious diseases in children[J]. CJCP, 2011, 13(3): 205-207.
［2］Carballeira NM. New advances in fatty acids as antimalarial, antimycobacterial and antifungal agents［J］. Prog Lipid Res, 2008, 47(1): 50-61.
［3］Murata A, Okuyama K, Sakano S, Kajiki M, Hirata T, Yagita H, et al. A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells［J］. J Immunol, 2010, 185(7): 3905-3912.
［4］Nickoloff BJ, Osborne BA, Miele L. Notch signaling as a therapeutic target in cancer: a new approach to the development of cell fate modifying agents［J］. Oncogene, 2003, 22(42): 6598-6608.
［5］Conboy IM, Conboy MJ, Smythe GM, Rando TA. Notchmediated restoration of regeneration potential to aged muscle［J］. Science, 2003, 302(5650): 1575-1577.
［6］Han W, Ye Q, Moore MA. A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells［J］. Blood, 2000, 95(5): 1616-1625.
［7］Hozumi K, Negishi N, Suzuki D, Abe N, Sotomaru Y, Tamaoki N, et al. Delta-like 1 is necessary for the generation of marginal zone B cell but not T cells in vivo［J］. Nature Immunol, 2004, 5(6): 638-644.
［8］Liu RZ, Mita R, Beaulieu M, Gao ZH, Godbout R. Fatty acid binding proteins in brain development and disease［J］. Int J Dev Biol, 2010, 54(8): 1229-1239.
［9］Kapoor N, Narayana Y, Patil SA, Balaji KN. Nitric oxide is involved in Mycobacterium bovis bacillus Calmette-Guérin-activated Jagged1 and Notch1 signaling［J］. J Immunol, 2010, 184(6): 3117-3126.
［10］Visan I, Yuan JS, Tan JB, Cretegny K, Guidos CJ. Regulation of intrathymic T-cell development by Lunatic Fringe-Notch1 interactions［J］. Immunol Rev, 2006, 209: 76-94.
［11］Wilson A, Ferrero I, MacDonald HR, Radtke F. Cutting edge: an essential role for Notch-1 in the development of both thymus-independent and-dependent T cells in the gut［J］. J Immunol, 2000, 165(10): 53975400.
［12］Dorsch M, Zheng G, Yowe D, Rao P, Wang Y, Shen Q, et al. Ectopic expression of Delta4 impairs hematopoietic development and leads to lymphoproliferative disease［J］. Blood, 2002, 100(6): 2046-2055.
［13］Wilson A, MacDonald HR, Radtke F. Notch 1-deficient common lymphoid precursors adopt a B cell fate in the thymus［J］. J Exp Med, 2001, 194(7): 1003-1012.
