Abstract：OBJECTIVE: To study the levels of CD4+CD25+CD127- and CD3+CD4-CD8- regulatory T (Treg) cells in peripheral blood of children with idiopathic thrombocytopenic purpura (ITP). METHODS: The flow cytometry was used to detect the expression of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells in peripheral blood of 33 children with ITP and 21 healthy children. RESULTS: The expression levels of CD4+CD25+CD127-［(2.7±1.7)% vs (4.8±1.6)%; P<0.01］ and CD3+CD4-CD8-［(5.2±3.1)% vs (8.1±3.5)%; P<0.01］Treg cells in children with ITP were significantly lower than in the controls. CONCLUSIONS: The expression levels of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells decrease in children with ITP, suggesting that CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells might play a role in the pathogenesis of ITP.
WANG Xue-Ping,QIU Yun-Shu,HAO Guo-Ping et al. Levels of regulatory T cells in peripheral blood of children with idiopathic thrombocytopenic purpura[J]. CJCP, 2011, 13(4): 282-284.
［4］Fontenot JD, Rudensky AY. A well adapted regulatory contriveance : regulatory T cell development and the forkhead family transcription factor Foxp3［J］.NAT Immunol, 2005, 6(4): 331337.
［5］Liu W, Putnam AL, Xu-Yu Z, Szot GL, Lee MR, Zhu S, et al. CDl27 expression inversely correlates with FoxP3 and suppressive function of human CD4+T reg cells［J］.J Exp Med, 2006, 203（7）: 1701-1711.
［7］Sakakura M, Wada H, Tawara I, Nobori T, Sugiyama T, Sagawa N, et al. Reduced Cd4+Cd25+ T cells in patients with idiopathic thrombocytopenic purpura［J］. Thromb Res, 2007, 120(2): 187-193.
［9］Young KJ, DuTemple B, Phillips MJ, Zhang L. Inhibition of graft-versus-host disease by double-negative regulatory T cells［J］. J Immunol, 2003, 171(1): 134-141.
［10］Hodak E, David M, Maron L, Aviram A, Kaganovsky E, Feinmesser M. CD4/CD8 double-negative epidermotropic cutaneous T-cell lymphoma: an immunohistochemical variant of mycosis fungoides［J］.J Am Acad Dermatol, 2006, 55(2): 276-284.
［11］Chen W, Ford MS, Young KJ, Zhang L. The role and mechanisms of double negative regulatory T cells in the suppression of immune rsponses［J］．Cell Mol Immunol, 2004, 1(5): 328-335.
［12］Yonezawa A, Onaka T, Imada K. Cytomegalovirus-associated infectious mononucleosis-like syndrome accompanied by transient monoclonal expansion of CD8+T-cells［J］. Rinsho Ketsueki, 2009, 50(8): 652-657.
