目的：探讨再生障碍性贫血（AA）患儿外周血 CD4+ CD25int/high CD127low 调节性 T 细胞（Treg）的表达特点及其与 Hb、WBC 和 血小板（Plt）数量的相关性。方法：采用流式细胞术检测 15 例正常儿童（对照组）和 22 例治疗有效的AA患儿治疗前后外周血 CD4+ CD25int/high CD127low Treg百分比的变化，分析 CD4+CD25high-CD127low Treg与 Hb、WBC 和 Plt 数量的相关性。结果：AA 组急性期外周血 CD4+CD25+、CD4+CD25high、CD4+CD25+CD127low、CD4+CD25highCD127low Treg占 CD4+ T 细胞的百分比均较对照组显著降低（P﹤0.05）；恢复期的表达则高于急性期（P﹤0.05），而与对照组比较差异无统计学意义（P﹥0.05）。AA组急性期、恢复期及对照组的 CD4+CD25highCD127low Treg表达率与外周血中 Hb、WBC 和 Plt 数量均呈正相关（r分别为 0.499、0.526、0.540，P<0.05）。结论：CD4+CD25int/highCD127low Treg 减低可能与 AA 发病有关，为其作为判断 AA 病情变化的指标提供了可能的理论依据，并可以作为免疫治疗的靶点之一进行深入研究。
Abstract
OBJECTIVE: To study the expression of CD4+ CD25int/high CD127low regulatory T cells in peripheral blood (PB) and its relation to the quantity of Hb, WBC and platelet (Plt) in children with aplastic anemia (AA). METHODS: Expression of CD4+ CD25int/high CD127low in PB was detected by flow cytometry in 22 children with AA before and after treatment and in 15 healthy controls. The relationships between CD4+CD25highCD127low and the quantity of Hb, WBC and Plt were evaluated. RESULTS: Compared to controls, the percentages of CD4+ CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ in PB of AA patients decreased markedly at the active phase (P﹤0.05) . By the recovery phase, the percentages of CD4+CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ increased significantly to the levels similar to the controls. There were significant positive relationships between the expression of CD4+CD25highCD127low cells and the quantity of Hb, WBC and Plt (r=0.499, 0.526, 0.540 respectively; P﹤0.05). CONCLUSIONS: The decrease of the percentage of CD4+CD25int/highCD127low regulatory T cells might be associated with the development of pediatric AA. The CD4+CD25int/highCD127low regulatory T cells can serve as a marker for the evaluation of disease severity as well as a target of further study on immune treatment of AA.
关键词
再生障碍性贫血 /
调节性 T 细胞 /
儿童
Key words
Aplastic anemia /
Regulatory T cell /
Child
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］Young NS. Hematopoietic cell destruction by immune mechanism in acquired aplastic anemia［J］. Semin Hematol, 2000, 37(1): 3-14.
［2］Stephens LA, Barclay AN, Mason D. Phenotypic characterization of regulatory CD4+CD25+ T cells in rats［J］. Int Immunol, 2004, 16(2): 365-375.
［3］中华医学会儿科分会血液组,中华儿科杂志编委会.小儿再生障碍性贫血的诊疗建议［J］.中华儿科杂志,2001,39(7):422-423.
［4］Lutsiak ME, Semnani RT, De Pascalis R, Kashmiri SV, Schlom J, Sabzevari H. Inhibition of CD4+25+ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide［J］.Blood, 2005, 105(7): 2862-2868.
［5］Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, et al. Cord blood CD4+CD25+ -derived T regulatory cells lines express FOXP3 protein and manifest potent suppressor function［J］.Blood, 2005, 105(2):750-758.
［6］Toda A, Piccirillo CA. Development and function of naturally occurring CD4+CD25+ regulatory T cells［J］.J Leukoc Biol, 2006, 80(3): 458-470.
［7］Kamikozuru K, Fukunaga K, Hirota S, Hida N, Ohda Y, Yoshida K, et al. The expression profile of functional regulatory T cells,CD4+CD25high+/forkhead box protein P3+, in patients with ulcerative colitis during active and quiescent disease［J］. Clin Exp Immunol, 2009, 156(2): 320-327.
［8］王盈，周磊明，宫菊丽，周明奎，沈方伟，郑晓虹，等.中国健康人外周血中具有CD4+CD25int/highCD127low特征的调节性T细胞频率［J］.细胞与分子免疫学杂志, 2007, 23(9): 816-818.
［9］王西阁,王晓格,胡姬婷. 调节性T细胞及Foxp3基因在再生障碍性贫血患儿外周血中的变化及意义［J］．中国当代儿科杂志，2010，12（4）：241-243.
［10］Hartigan-O'Connor DJ, Poon C, Sinclair E, McCune JM. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127),allowing consistent identification and sorting of live cells［J］. J Immunal Methods, 2007, 319(1-2): 41-52.
［11］韩文敏，邱国强，周民，任敏，谢晓宝. CD4+CD25highCD127low Tregs 及 TGF-β 在多发性骨髓瘤患者外周血的表达分析［J］.细胞与分子免疫学杂志,2008,24(10):996-998.
［12］Moniuszko M, Bodzenta-Lukaszyk A, Dabrowska M. Effects of oral glucocorticoid therapy on CD4+CD25+CD127- and CD4+CD25high T cell levels in asthmatic patients［J］. Inflammation, 2010, 33(6): 415-420.
PDF(943 KB)
