Abstract:OBJECTIVE: To explore the viral etiology of acute low respiratory tract infection (ALRTI) among hospitalized children in Changsha of Hunan Province of China. METHODS: Nasopharyngeal aspirates were collected from 1165 hospitalized children with ALRTI in Changsha from September 2007 to August 2008. Respiratory syncytin virus (RSV), human rhinovirus (HRV), influenza virus A (IFVA), influenza virus B (IFVB), parainfluenza 1-3 (PIV 1-3), human metapneumovirus (hMPV), human coronaviruses NL63 (HCoV-NL63), and human coronaviruses HKU1 (HCoVHKU1) were detected by reverse transcription polymerase chain reaction (RT-PCR). Adenovirus (ADV) and human bocavirus (HBoV) were detected by standard polymerase chain reaction (PCR). WU polyomaviruses (WUPyV) and KI polyomaviruses(KIPyV) were detected by nested PCR. The positive samples further underwent genetic sequencing. RESULTS: Among the 1165 nasopharyngeal aspirates, viruses were detected in 871 samples (74.76%), among which RSV (27.03%) was the most common virus, followed by HRV (17.33%), PIV3(13.73%), HBoV (8.67%) and hMPV (6.52%). The overall positive rate of viral detection showed no significant differences between males and females (χ2=2.241, P=0.134), whereas the positive rates of PIV3, hMPV, and HBoV in males were higher than in females. The positive rate of viral detection showed significant differences among different age groups (χ2=10.934, P=0.027), and the highest positive rate was noted in the age group of 6 months to 1 year. Furthermore, the overall positive rate of viral detection showed a significant difference in term of seasonal distribution, with a peak prevalence in winter. CONCLUSIONS: Virues predominate in the etiology of pediatric ALRTI in Changsha, and RSV, HRV and PIV3 are the main viruses for ALRTI. HBoV and hMPV have become increasingly important. Viral infection-associated ALRTI shows a prevail in the age group of 6 months to 1 year as well as in winter.
[7]Zhang RF, Jin Y, Xie ZP, Liu N, Yan KL, Gao HC, et al. Human respiratory syncytial virus in children with acute respiratory tract infections in China[J]. J Clin Microbiol, 2010, 48(11): 4193-4199.
[11]Jin Y, Yuan XH, Xie ZP, Gao HC, Song JR, Zhang RF, et al. Prevalence and clinical characterization of the newly putative human rhinovirus C species in children with acute respiratory tract infection[J]. J Clin Microbiol, 2009, 47(9): 2895-2900.
[12]Henrickson KJ, Hoover S, Kehl KS, Hua W. National disease burden of respiratory viruses detected in children by polymerase chain reaction[J]. Pediatr Infect Dis,2004,23(1):11-18.
[14]Wubbel L, Muniz L, Ahmed A, Trujillo M, Carubelli C, McCoig C, et al. Etiology and treatment of community acquired pneumonia in ambulatory children[J]. Pediatr Infect Dis, 1999,18(2):98-104.
[15]Jeffrey S Kahn. Epidemiology of human Metapneumovirus[J]. J Clin Microbiol, 2006, 19(3): 546-557.
[19]Woo PC, Lau SK, Chu CM, Chan KH, Tsoi HW, Huang Y, et al.Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia[J]. J Virol, 2005, 79(2): 884-895.
[21]Lau SK, Woo PC, Yip CC, Tse H, Tsoi HW, Cheng VC. Coronavirus HKU1 and other Coronavirus Infections in Hong Kong[J]. J Clin Micr, 2006, 44(6): 2063-2071.
[22]Bialasiewicz S, Whiley DM, Lambert SB, Wang D, Nissen MD, Sloots TP. A newly reported human polyomavirus, KI virus, is present in the respiratory tract of Australian children[J]. J Clin Virol, 2007, 40(1): 15-18.
[23]Han TH, Chung JY, Koo JW, Kim SW, Hwang ES. WU polyomavirus in children with acute lower respiratory tract infections, South Korea[J]. Emerg Infect Dis, 2007,13(11): 1766-1768.
[26]Wattier RL, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, et al. Role of human polyomaviruses in respiratory tract disease in young children[J]. Emerg Infect Dis, 2008, 14(11): 1766-1768.
[27]Kesebir D, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, et al. Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus[J]. J Infect Dis, 2006,194(9): 1276-1282.
[28]Maggi F, Andreoli E, Pifferi M, Meschi S, Rocchi J, Bendinelli M. Human bocavirus in Italian patients with respiratory diseases[J]. J Clin Virol, 2007, 38(4): 321-325.
[29]Fry AM, Lu X, Chittaganpitch M, Peret T, Fischer J, Dowell SF, et al. Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand[J]. J Infect Dis, 2007, 195(7): 1038-1045.
[31]Zheng LS, Yuan XH, Xie ZP, Jin Y, Gao HC, Song JR, et al. Human bocavirus infection in young children with acute respiratory tract infection in Lanzhou, China[J]. J Med Virol, 2010, 82(2): 282-288.