Etiology of nonspecific chronic cough in children and relationship between TRPV1 gene polymorphisms and nonspecific chronic cough
ZHANG Xiao-Ning, YANG Juan, LUO Zheng-Xiu, LUO Jian, REN Luo, LI Bo, CHEN Kun-Hua, FU Zhou, LU Quan, LIU En-Mei
Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing 400014, China. emliu186@hotmail.com
Abstract：OBJECTIVE: To explore the causes of nonspecific chronic cough in children and relationship between transient receptor potential vanilloid 1 (TRPV1) gene polymorphisms and nonspecific chronic cough. METHODS: A total of 195 children with chronic cough were followed up half a month, one month and three months after their first visit to hospital. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine polymorphisms of the TRPV1 gene in the children. A total of 205 healthy or surgical children without chronic cough served as the control group. RESULTS: The etiologic distribution of the 195 children with chronic cough was as follows: 96 (49.2%) cases of cough variant asthma (CVA), 48 (24.6%) cases of CVA complicated by upper airway cough syndrome (UACS), 34 (17.4%) cases of post-infectious cough, and 17 (8.7%) cases of UACS. Three genotypes were identified in both groups at positions rs222747 (CC, GC and GG), rs222748 (CC, TC and TT) and rs8065080 (CC, TC and TT). The frequencies of genotype and allele at position rs222747 did not accord with the law of Hardy-Weinberg. There was no significant difference in frequencies of genotype and allele at positions rs222748 and rs8065080 between the two groups. CONCLUSIONS: CVA, UACS and post-infectious cough are common causes of nonspecific chronic cough in children. TRPV1 gene polymorphisms at positions rs222748 and rs8065080 may be unrelated to nonspecific chronic cough in children.
ZHANG Xiao-Ning,YANG Juan,LUO Zheng-Xiu et al. Etiology of nonspecific chronic cough in children and relationship between TRPV1 gene polymorphisms and nonspecific chronic cough[J]. CJCP, 2012, 14(07): 524-528.
［2］Leonardi GS, Houthuijs D, Nikiforov B, Volf J, Rudnai P, Zejda J, et al. Respiratory symptoms, bronchitis and asthma in children of Central and Eastern Europe［J］. Eur Respir J, 2002, 20(4): 890-898.
［3］Lee LY, Ni D, Hayes D Jr, Lin RL. TRPV1 as a cough sensor and its temperature-sensitive properties［J］. Pulm Pharmacol Ther, 2011, 24(3): 280-285.
［6］Cantero-Recasens G, Gonzalez JR, Fandos C, Duran-Tauleria E, Smit LA, Kauffmann F, et al. Loss of function of transient receptor potential vanilloid 1(TRPV1) genetic variant is associated with lower risk of active childhood asthma［J］. J Biol Chem, 2010, 285(36): 27532-27535.
［8］Lee LY, Pisarri T. Afferent properties and reflex functions of bronchopulmonary C-fibers［J］. Respir Physiol, 2001, 125(1-2): 47-65.
［9］Mitchell JE, Campbell AP, New NE, Sadofsky LR, Kastelik JA, Mulrennan SA, et al. Expression and characterisation of the intracellular vanilloid receptor (TRPV1) in bronchi from patients with chronic cough［J］. Exp Lung Res, 2005, 31(3): 295-306.
［10］Groneberg DA, Niimi A, Dinh QT, Cosio B, Hew M, Fischer A, et al. Increased expression of transient receptor potential vallinoid-1 in airway nerves of chronic cough［J］. Am J Respir Crit Care Med, 2004, 170(12):1276-1280.
［11］Veronesi B, Oortgiesen M. The TRPV1 receptor: target of toxicants and therapeutics［J］. Toxicol Sci, 2006, 89(1):1-3.
