孟鲁司特对幼年哮喘气道重塑大鼠感觉神经肽受体NK1R表达的影响

doi:10.7499/j.issn.1008-8830.2013.04.013

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摘要目的：探讨孟鲁司特对幼年哮喘气道重塑大鼠中感觉神经肽受体NK1R表达的影响。方法：24只Sprague-Dawley（SD）大鼠随机分为对照组、哮喘组和孟鲁司特组，每组8只。应用卵白蛋白（OVA）雾化吸入法建立哮喘大鼠模型，对照组大鼠应用生理盐水代替致敏液和1%OVA，孟鲁司特组于每次激发前2 h以孟鲁司特（15 mg/kg）灌胃，共持续8 周。采用免疫组化、real-time PCR和Western blot等方法，从mRNA和蛋白水平动态观察NK1R在哮喘大鼠气道重塑中的表达水平及孟鲁司特对其表达的影响。结果：哮喘组NK1R mRNA及蛋白的表达水平均较对照组明显增加，差异有统计学意义(P<0.01)；孟鲁司特干预后NK1R mRNA及蛋白表达水平较哮喘组减少(P<0.05)，但仍高于对照组(P<0.01)。结论哮喘：诱发大鼠气道NK1R的表达增加，孟鲁司特能够下调哮喘大鼠气道重塑中NK1R的表达水平。

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	魏兵
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关键词 ：哮喘, 气道重塑, 感觉神经肽受体, 孟鲁司特, 大鼠

Abstract：OBJECTIVE: To investigate the effect of montelukast on the expression of sensory neuropeptide (neurokinin-1) receptor (NK1R) in young asthmatic rats with airway remodeling. METHODS: Twenty-four Sprague-Dawley rats were randomly divided into control group (n=8), asthma (n=8), and montelukast groups (n=8). A rat model of asthma was induced by ovalbumin (OVA) inhalation. Normal saline was used instead of sensitizing solution and 1% OVA in the control group. Each rat in the montelukast group was given montelukast (15 mg/kg) by gavage 2 h before OVA inhalation. All rats received their respective treatments for 8 weeks. Immunohistochemistry, real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NK1R in asthmatic airway remolding and to evaluate the effect of montelukast on NK1R expression. RESULTS: The asthma group showed significantly higher mRNA and protein expression levels of NK1R than the control group (P<0.01). The mRNA and protein expression levels of NK1R in the montelukast group were significantly lower than in the asthma group (P<0.05), but significantly higher than in the control group (P<0.01). CONCLUSIONS: Rats with induced asthma have upregulated NK1R expression in the airway, and montelukast can downregulate NK1R expression during airway remodeling.

Key words： Asthma Airway remodeling Sensory neuropeptide receptor Montelukast Rats

引用本文:

魏兵,尚云晓,李淼等. 孟鲁司特对幼年哮喘气道重塑大鼠感觉神经肽受体NK1R表达的影响[J]. 中国当代儿科杂志, 2013, 15(4): 298-301.

WEI Bing,SHANG Yun-Xiao,LI Miao et al. Effect of montelukast on the expression of neurokinin-1 receptor in young asthmatic rats with airway remodeling[J]. CJCP, 2013, 15(4): 298-301.

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http://www.zgddek.com/CN/10.7499/j.issn.1008-8830.2013.04.013 或 http://www.zgddek.com/CN/Y2013/V15/I4/298

	［1］Tagaya E, Tamaoki J. Mechanisms of airway remodeling in asthma［J］. Allergol Int， 2007,56(4):331-340.
	［2］尚云晓,蔡栩栩,韩晓华,赵淑琴,孔淑卿,魏克伦.神经激肽A在哮喘儿童血浆含量变化的动态研究［J］.中华儿科杂志，2003,41(6):457-459.
	［3］尚云晓,韩晓华,赵淑琴, 蔡栩栩.神经激肽B在支气管哮喘中的含量变化［J］. 中国医科大学学报，2003,32(6):481-482.
	［4］尚云晓,王艳,蔡栩栩,赵淑琴,魏克伦.烟雾暴露对哮喘豚鼠肺组织中P物质含量及基因表达的影响［J］.中华结核和呼吸杂志，2004,27(4):279-280.
	［5］尚云晓,张晗,韩晓华,赵淑琴.糖皮质激素对哮喘豚鼠血浆、肺组织中神经激肽A含量的影响及其分子机制［J］.中华儿科杂志，2005,43(6):418-420.
	［6］李淼,尚云晓.哮喘豚鼠气道内NK1R mRNA表达及其含量的研究［J］. 中国医科大学学报, 2005，34(6): 502-504.
	［7］梁亚峰,张维溪,李昌崇,王小明,革丽莎.哮喘大鼠气道重塑中尾加压素2Ⅱ的表达变化［J］. 中国当代儿科杂志，2010,12 (4):287-289.
	［8］栾斌,冯晓霞,杨玉霞,张志英.FIZZ1及NOTCH1在哮喘中的作用［J］. 中国当代儿科杂志，2011,13 (3):219-222.
	［9］魏兵,尚云晓,李淼,张晗,李娜.吸入布地奈德对幼年大鼠气道重塑的影响［J］.中国美容医学，2011，20(6):76-78.
	［10］魏兵,尚云晓,李淼,张晗,张克,马明.孟鲁司特对幼年大鼠气道重塑的影响［J］.儿科药学杂志，2012,18(3):5-7.
	［11］Vanacker NJ, Palmans E, Kips JC, Pauwels RA. Fluticasone inhibits but does not reverse allergen induced structural airway changes［J］. Am J Respir Crit Care Med, 2001, 163 (3 Pt 1): 674-679.
	［12］Mehrotra AK, Henderson WR Jr. The role of leukotrienes in airway remodeling［J］. Curr Mol Med, 2009, 9(3): 383-391.
	［13］Bossé Y, Stankova J, Rola-Pleszczynski M. Cysteinyl leukotrienes in asthmatic airway smooth muscle cell hyperplasia［J］. Ann Allergy Asthma Immunol, 2009, 102(1): 16-21.
	［14］Potter-Perigo S, Baker C, Tsoi C, Braun KR, Isenhath S, Altman GM, et al. Regulation of proteoglycan synthesis by leukotriene d4 and epidermal growth factor in bronchial smooth muscle cells［J］. Am J Respir Cell Mol Biol, 2004, 30(1): 101-108.
	［15］Henderson WR Jr, Tang LO, Chu SJ, Tsao SM, Chiang GK, Jones F, et al. A role for cysteinyl leukotrienes in airway remodeling in a mouse asthma model［J］. Am J Respir Crit Care Med, 2002, 165(1): 108-116.
	［16］Muz MH, Deveci F, Bulut Y. Ilhan N, Yekeler H, Turgut T. The effects of low dose leukotriene receptor antagonist therapy on airway remodeling and cysteinyl leukotriene expression in a mouse asthma model［J］. Exp Mol Med, 2006, 38(2): 109-118.