左旋精氨酸对低出生体重仔鼠PI3K和PKB的影响

doi:10.7499/j.issn.1008-8830.2013.08.018

摘要
图/表
参考文献(30)
相关文章 (15)
点击分布统计
下载分布统计

全文: PDF (735 KB)
输出: BibTeX | EndNote (RIS) 背景资料

摘要目的：生命早期应用左旋精氨酸（L-Arg）干预低出生体重仔鼠，检测其肝脏磷脂酰肌醇-3-激酶（PI3K）和蛋白激酶B(PKB)的表达，探讨L-Arg对改善胰岛素抵抗的影响。方法：以孕期低蛋白饮食法建立低出生体重仔鼠模型，18只孕鼠随机分为对照组、模型组和干预组，每组6只。对照组孕期给予21%正常蛋白饲料建立正常出生体重仔鼠模型，模型组及干预组孕期给予10%低蛋白饲料建立低出生体重仔鼠模型，3组孕鼠所生仔鼠分别纳入仔鼠对照组、模型组、干预组。生后21 d 仔鼠的哺乳期内，3组孕鼠均给予21%正常蛋白饲料喂养，对照组及模型组给予正常饮用水喂养，干预组给予富含L-Arg（200 mg/kg.d）的饮用水喂养。断乳后，3组仔鼠均给予21%正常蛋白饲料及正常饮用水喂养。于仔鼠生后1 周、3 周、8 周时，分别留取3组仔鼠的肝脏标本，用Western blot法检测肝脏PI3K和PKB的蛋白表达。结果：1 周时干预组仔鼠肝脏PI3K的蛋白表达高于模型组（P=0.045），且与正常组相比差异无统计学意义（P=0.503）。8 周时干预组仔鼠肝脏PKB蛋白表达明显高于模型组（P=0.039），且与正常组比较差异无统计学意义（P>0.05）。结论：生命早期补充L-Arg可促进蛋白质的合成，增加肝脏PI3K及PKB的蛋白表达，促进胰岛素信号传导，改善胰岛素抵抗。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	罗开菊
	陈平洋
	谢宗德
	李雯
	李素萍
	贺鸣凤

关键词 ：左旋精氨酸, 低出生体重, 肝脏, 胰岛素信号传导, 胰岛素抵抗, 大鼠

Abstract：OBJECTIVE: To measure the expression of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (PKB) in liver tissue among low-birth-weight newborn rats treated with L-arginine (L-Arg) in early life, and to investigate the effect of L-Arg on insulin resistance. METHODS: Eighteen pregnant rats were randomly divided into three groups: control, model and intervention (n=6 each). The control group was fed with normal protein feed (protein content=21%) during pregnancy to establish a normal-birth-weight newborn rat model, and the model and intervention groups were fed with low-protein feed (protein content=10%) during pregnancy to establish a low-birth-weight newborn rat model. Newborn rats from the three pregnant rat groups were also assigned to control, model and intervention groups. During 21 days of lactation, maternal rats in the control and model groups were fed with normal protein feed and normal drinking water, while maternal rats in the intervention group were fed with normal protein feed and drinking water rich in L-Arg (200 mg/kg·d). After ablactation, the three groups of newborn rats were fed with normal protein feed and normal drinking water. Liver tissue samples were collected from these newborn rats at 1, 3 and 8 weeks after birth. Protein expression of PI3K and PKB in liver tissue was measured by Western blot. RESULTS: At 1 week after birth, the newborn rats in the intervention group had significantly higher protein expression of PI3K than in the model group (P=0.045), but there was no significant difference when compared with the control group (P=0.503). At 8 weeks after birth, the newborn rats in the intervention group had significantly higher protein expression of PKB than the model group (P=0.039), but there was no significant difference when compared with the control group (P>0.05). CONCLUSIONS: A supplement of L-Arg in early life can boost protein synthesis, increase protein expression of PI3K and PKB in liver tissue, promote insulin signaling and reduce insulin resistance.

Key words： L-arginine Low birth weight Liver Insulin signaling Insulin resistance Rats

引用本文:

罗开菊,陈平洋,谢宗德等. 左旋精氨酸对低出生体重仔鼠PI3K和PKB的影响[J]. 中国当代儿科杂志, 2013, 15(8): 682-685.

LUO Kai-Ju,CHEN Ping-Yang,XIE Zong-De et al. Effects of L-Arg on expression of PI3K and PKB in liver among low-birth-weight newborn rats[J]. CJCP, 2013, 15(8): 682-685.

链接本文:

http://www.zgddek.com/CN/10.7499/j.issn.1008-8830.2013.08.018 或 http://www.zgddek.com/CN/Y2013/V15/I8/682

[5]	Hemmings BA, Restuccia DF. PI3K-PKB/Akt pathway[J]. Cold Spring Harb Perspect Biol, 2012, 4(9): a011189.
[1]	Rinaudo P, Wang E. Fetal programming and metabolic syndrome[J]. Annu Rev Physiol, 2012, 74: 107-130.
[2]	Schultze SM, Jensen J, Hemmings BA, Tschopp O, Niessen M. Promiscuous affairs of PKB/AKT isoforms in metabolism[J]. Arch Physiol Biochem, 2011, 117(2): 70-77.
[6]	Sun Y, Liu S, Ferguson S, Wang L, Klepcyk P, Yun JS, et al. Phosphoenolpyruvate carboxykinase overexpression selectively attenuates insulin signaling and hepatic insulin sensitivity in transgenic mice[J]. J Biol Chem, 2002, 277(26): 23301-23307.
[7]	李瑶, 辛颖. 胰岛素受体底物在宫内发育迟缓大鼠胰腺组织中的表达[J]. 中国当代儿科杂志, 2010, 12(12): 972-975.
[3]	Xie Z, Dong Q, Ge J, Chen P, Li W, Hu J. Effect of low birth weight on impaired renal development and function and hypertension in rat model[J]. Ren Fail, 2012, 34(6): 754-759.
[8]	Andreelli F, Laville M, Ducluzeau PH, Vega N, Vallier P, Khalfallah Y, et al. Defective regulation of phosphatidylinositol-3-kinase gene expression in skeletal muscle and adipose tissue of non-insulin-dependent diabetes mellitus patients[J]. Diabetologia, 1999, 42(3): 358-364.
[4]	Lucas A. Programming by early nutrition: an experimental approach[J]. J Nutr, 1998, 128(2 Suppl): 401S-406S.
[5]	Hemmings BA, Restuccia DF. PI3K-PKB/Akt pathway[J]. Cold Spring Harb Perspect Biol, 2012, 4(9): a011189.
[6]	Sun Y, Liu S, Ferguson S, Wang L, Klepcyk P, Yun JS, et al. Phosphoenolpyruvate carboxykinase overexpression selectively attenuates insulin signaling and hepatic insulin sensitivity in transgenic mice[J]. J Biol Chem, 2002, 277(26): 23301-23307.
[7]	李瑶, 辛颖. 胰岛素受体底物在宫内发育迟缓大鼠胰腺组织中的表达[J]. 中国当代儿科杂志, 2010, 12(12): 972-975.
[9]	王涤非, 蒋丽娟, 张锦, 刘国良. 2型糖尿病小鼠骨骼肌细胞受体后胰岛素信号转导蛋白的下降调节[J]. 山西医药杂志, 2006(1): 10-12.
[8]	Andreelli F, Laville M, Ducluzeau PH, Vega N, Vallier P, Khalfallah Y, et al. Defective regulation of phosphatidylinositol-3-kinase gene expression in skeletal muscle and adipose tissue of non-insulin-dependent diabetes mellitus patients[J]. Diabetologia, 1999, 42(3): 358-364.
[9]	王涤非, 蒋丽娟, 张锦, 刘国良. 2型糖尿病小鼠骨骼肌细胞受体后胰岛素信号转导蛋白的下降调节[J]. 山西医药杂志, 2006(1): 10-12.
[10]	Martin-Gronert MS, Ozanne SE. Mechanisms underlying the developmental origins of disease[J]. Rev Endocr Metab Disord, 2012, 13(2): 85-92.
[10]	Martin-Gronert MS, Ozanne SE. Mechanisms underlying the developmental origins of disease[J]. Rev Endocr Metab Disord, 2012, 13(2): 85-92.
[11]	Soumaya K. Molecular mechanisms of insulin resistance in diabetes[J]. Adv Exp Med Biol, 2012, 771: 240-251.
[12]	刘晓梅, 焦伊胜, 潘莉莉, 卢岩, 李书琴. 宫内生长受限大鼠肝脏磷酸肌醇-3-激酶信号通路分子的变化[J]. 中国当代儿科杂志, 2009, 3(3): 221-224.
[11]	Soumaya K. Molecular mechanisms of insulin resistance in diabetes[J]. Adv Exp Med Biol, 2012, 771: 240-251.
[12]	刘晓梅, 焦伊胜, 潘莉莉, 卢岩, 李书琴. 宫内生长受限大鼠肝脏磷酸肌醇-3-激酶信号通路分子的变化[J]. 中国当代儿科杂志, 2009, 3(3): 221-224.
[13]	Escudero A, Petzold G, Moreno J, Gonzalez M, Junod J, Aguayo C, et al. Supplementation with apple enriched with L-arginine may improve metabolic control and survival rate in alloxan-induced diabetic rats[J]. Biofactors, 2013[Epub ahead of print].
[13]	Escudero A, Petzold G, Moreno J, Gonzalez M, Junod J, Aguayo C, et al. Supplementation with apple enriched with L-arginine may improve metabolic control and survival rate in alloxan-induced diabetic rats[J]. Biofactors, 2013[Epub ahead of print].
[14]	Marchetti P, Bugliani M, Boggi U, Masini M, Marselli L. The pancreatic beta cells in human type 2 diabetes[J]. Adv Exp Med Biol, 2012, 771: 288-309.
[15]	卢岩，刘晓梅，李书琴. L-精氨酸对宫内发育迟缓胎鼠胰岛素样生长因子及其结合蛋白表达的影响[J]. 中国当代儿科杂志, 2006, 7(4): 319-322.
[16]	Fu WJ, Haynes TE, Kohli R, Hu J, Shi W, Spencer TE, et al. Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats[J]. J Nutr, 2005, 135(4): 714-721.
[14]	Marchetti P, Bugliani M, Boggi U, Masini M, Marselli L. The pancreatic beta cells in human type 2 diabetes[J]. Adv Exp Med Biol, 2012, 771: 288-309.
[15]	卢岩，刘晓梅，李书琴. L-精氨酸对宫内发育迟缓胎鼠胰岛素样生长因子及其结合蛋白表达的影响[J]. 中国当代儿科杂志, 2006, 7(4): 319-322.
[16]	Fu WJ, Haynes TE, Kohli R, Hu J, Shi W, Spencer TE, et al. Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats[J]. J Nutr, 2005, 135(4): 714-721.
[17]	Feng Y, Li J, Yang J, Yang Q, Lv Q, Gao Y, et al. Synergistic effects of taurine and L-arginine on attenuating insulin resistance hypertension[J]. Adv Exp Med Biol, 2013, 775: 427-435.
[17]	Feng Y, Li J, Yang J, Yang Q, Lv Q, Gao Y, et al. Synergistic effects of taurine and L-arginine on attenuating insulin resistance hypertension[J]. Adv Exp Med Biol, 2013, 775: 427-435.