Abstract：Objective To study the role of circulating microRNAs (miRNA) in the pathogenesis of idiopathic short stature (ISS) through detecting miRNA expression profile in plasma of children with ISS. Methods Plasma miRNA expression was determined by microarray in 20 children with ISS and 20 healthy children. Altered microRNAs were verified by real-time PCR. The online miRNA target gene prediction software was used to predict and screen miRNA differentially expressed target genes. Results According to the microarray, there were 40 differentially expressed miRNAs in the ISS group compared with the control group, including 24 up-regulated miRNAs and 16 down-regulated miRNAs. Real-time PCR verified two up-regulated (miR-185 and miR-574-5p) and two down-regulated miRNAs (miR-497 and miR-15a) and confirmed that plasma miR-185 expresson was significantly up-regulated (P<0.05) and miR-497 expression was significantly down-regulated (P<0.05) in children with ISS. Conclusions Plasma miRNA expression levels in children with ISS are significantly different from healthy controls, suggesting that plasma miRNA is associated with the pathogenesis of ISS.
Cohen P, Rogol AD, Deal CL, Saenger P, Reiter EO, Ross JL, et al. Consensus statement on the diognosis and treatment of children with idiopathic short stature: a summary of the growth hormone research society, the lawson wilkinspediatric endocrine society, and the European society for paediatric endocrinology workshop[J]. J Clin Endocrinol Metab, 2008, 93(11): 4210-4217.
Brase JC, Wuttig D, Kuner R, Sultmann H. Serum microRNAs as non-invasive biomarkers for cancer[J]. Molecular Cancer, 2010, 9:306.
[4]
Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, et al. Serum microRNAs are promising novel biomarkers[J]. PLoS One, 2008, 3(9): 3148.
[5]
Gui J, Tian Y, Wen X, Zhang W, Zhang P, Gao J, et al. Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies[J]. Clin Sci(Lond), 2011, 120(5): 183-193.
[6]
Ma Y, Wang X, Jin H. Methylated DNA and microRNA in Body Fluids as Biomarkers for Cancer Detection[J]. Int J Mol Sci, 2013, 4(5): 10307-10331.
[7]
Ji X, Takahashi R, Hiura Y, Hirokawa G, Fukushima Y, Iwai N. Plasma miR-208 as a biomarker of myocardial injury[J]. Clin Chem, 2009, 55(11): 1944-1949.
[8]
Wang K, Zhang S, Marzolf B, Troisch P, Brightman A, Hu Z, et al. Circulating microRNAs, potential biomarkers for drug-induced liver injury[J]. Proc Natl Acad Sci USA, 2009, 106(11): 4402-4407.
[9]
Vasilescu C, Rossi S, Shimizu M, Tudor S, Veronese A, Ferracin M, et al. MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis[J]. PLoS One, 2009, 4(10): e7405.
[10]
Kirschner MB, Edelman JJ, Kao SC, Vallely MP, van Zandwijk N, Reid G. The impact of hemolysis on cell-Free microRNA biomarkers[J]. Front Genet, 2013, 4: 94.
Lettre G. Genetic regulation of adult stature[J]. Curr Opin Pediatr, 2009, 21(4): 515-522.
[16]
Klammt J, Kiess W, Pfaffle R. IGF1R mutations as cause of SGA[J]. Best Pract Res Clin Endocrinol Metab, 2011, 25(1): 191.
[17]
Papaconstantinou J. Insulin/IGF-1 and ROS signaling pathway cross talk in aging and longevity determination[J]. Mol Cell Endocrirwl, 2009, 299(1): 89-100.
[18]
Hwa V, Little B, Adiyaman P, Kofoed EM, Pratt KL, Ocal G, et al. Severe growth hormone insensitivity resulting from total absence of signal transducer and activator of transcription 5b[J]. J Clin Endocrinol Metab, 2005, 90(70): 4260-4266.
