Abstract：Objective To study the association of ORMDL3 single nucleotide polymorphisms (SNP) with lysophosphatidylcholine (LysoPC) and apolipoprotein B (apoB) levels. Methods A total of 300 children diagnosed with bronchial asthma between January 2010 and December 2012 were selected for the asthma group, and 298 children diagnosed with upper respiratory tract infection in the same period were selected for the control group. Serum LysoPC and apoB levels were measured using enzyme-linked immunosorbent assay. Genotype analysis was performed using the TaqMan probe. Results LysoPC and apoB levels were significantly higher in the asthma group than in the control group (P<0.01). Among children with various genotypes of ORMDL3 gene at locus rs12603332, the asthma group had significantly higher LysoPC and apoB levels than the control group (P<0.01). Among the children with asthma, those with CC genotype had significantly higher LysoPC and apoB levels than those with CT and TT genotypes (P<0.01). Conclusions LysoPC and apoB may intervene in the pathological process of asthma. Pro-inflammatory gene ORMDL3 SNP rs12603332 may be associated with high LysoPC and apoB levels, which leads to the occurrence of childhood asthma.
WANG Bing-Jie,WANG Gui-Lan,CHEN De-Hui et al. Association of ORMDL3 single nucleotide polymorphisms with lysophosphatidylcholine and apolipoprotein B levels in children with asthma[J]. CJCP, 2015, 17(3): 241-244.
Wood LG, Garg ML, Gibson PG. A high-fat challenge increases airway inflammation and impairs bronchodilator recovery in asthma[J]. Allergy Clin Immunol, 2010, 127(5): 1133-1139.
[2]
Soto-Ramírez N, Karmaus W, Zhang H, et al. Fatty acids in breast milk associated with asthma-like symptoms and atopy in infancy: a longitudinal study[J]. Asthma, 2012, 49(9): 926-934.
[3]
Kim JH, Ellwood PE, Asher MI. Diet and asthma: looking back, moving forward[J]. Respir Res, 2009, 10: 49.
[4]
Thiara G, Goldman RD. Milk consumption and mucus production in children with asthma[J]. Can Fam Physician, 2012, 58(2): 165-166.
[5]
Chauffe RJ, Wilensky RL, Mohler ER 3rd. Recent developments with lipoprotein-associated phospholipase A2 inhibitors[J]. Curr Atheroscler Rep, 2010, 12(1): 43-47.
[6]
Breslow DK, Collins SR, Bodenmiller B, et al. Orm family proteins mediate sphingolipid homeostasis[J]. Nature, 2010, 463(7284): 1048-1053.
Nagel G, Weiland SK, Rapp K, et al. Association of apolipoproteins with symptoms of asthma and atopy among school children[J]. Int Arch Allergy Immunol, 2009, 149(3): 259-266.
[9]
Kang MJ, Yu HS, Seo JH, et al. GSDMB/ORMDL3 variants contribute to asthma susceptibility and eosinophil-mediated bronchial hyperresponsiveness[J]. Hum Immunol, 2012, 73(9): 954-959.
[10]
Yang FF, Huang Y, Li QB, et al. Single nucleotide polymorphisms in the ORM1-like 3 gene associated with childhood asthma in a Chinese population[J]. Genet Mol Res, 2012, 11(4): 4646-4653.
[11]
Moffatt MF, Kabesch M, Liang L, et al. Genetic variants regulating ORMDL3 expression contribute to the risk of childhood asthma[J]. Nature, 2007, 448(7152): 470-473.
Black PN, Sharpe S. Dietary fat and asthma: is there a connection?[J]. Eur Respir, 1997, 10(1): 6-12.
[15]
Kume H, Ito S, Ito Y, et al. Role of lysophosphatidylcholine in the desensitization of β-adrenergic receptors by Ca2+ sensitization in tracheal smooth muscle[J]. Am J Respir Cell Mol Biol, 2001, 25(3): 291-298.
[16]
Nishi E, Kume N, Ueno YH, et al. Lyso-phosphatidylcholine enhances cytokine-induced interferon gamma expression in human T lymphocytes[J]. Circ Res, 1998, 83(5): 508-515.
[17]
Kume N, Gimbrone MA Jr. Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells[J]. J Clin Invest, 1994, 93(2): 907-911.
[18]
Thompson A, Danesh J. Associations between apolipoprotein B, apolipoprotein AI, the apolipoprotein B/AI ratio and coronary heart disease: a literature-based meta-analysis of prospective studies[J]. Intern Med, 2006, 259(5): 481-492.
[19]
Jin R, Xu HG, Yuan WX, et al. Mechanisms elevating ORMDL3 expression in recurrent wheeze patients: role of Ets-1, p300 and CREB[J]. Int J Biochem Cell Biol, 2012, 44(7): 1174-1183.
