哮喘患儿ORMDL3基因多态性与溶血卵磷脂及载脂蛋白B的关系

王冰洁; 王桂兰; 陈德晖; 王文祥; 黄娟; 容嘉妍; 刘翔腾; 杨赛

doi:10.7499/j.issn.1008-8830.2015.03.008

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中国当代儿科杂志 ›› 2015, Vol. 17 ›› Issue (3) : 241-244. DOI: 10.7499/j.issn.1008-8830.2015.03.008

论著·临床研究

哮喘患儿ORMDL3基因多态性与溶血卵磷脂及载脂蛋白B的关系

王冰洁¹, 王桂兰¹, 陈德晖², 王文祥³, 黄娟¹, 容嘉妍¹, 刘翔腾¹, 杨赛¹

作者信息 +

Association of ORMDL3 single nucleotide polymorphisms with lysophosphatidylcholine and apolipoprotein B levels in children with asthma

WANG Bing-Jie¹, WANG Gui-Lan¹, CHEN De-Hui², WANG Wen-Xiang³, HUANG Juan¹, RONG Jia-Yan¹, LIU Xiang-Teng¹, YANG Sai¹

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文章历史 +

摘要

目的探讨哮喘患儿ORMDL3 基因多态性与溶血卵磷脂(Lyso-PC)及载脂蛋白B(apoB)的关系。方法选取2010 年1 月至2012 年12 月诊断为支气管哮喘的300 例患儿为哮喘组,另选取同期诊断为上呼吸道感染的298 例患儿为对照组。ELISA 法检测两组患儿血清中Lyso-PC 和apoB 水平;TaqMan 探针法进行基因型分析。结果哮喘组Lyso-PC、apoB 水平明显高于对照组(P<0.01);哮喘组ORMDL3 基因rs12603332 位点不同基因型患儿Lyso-PC、apoB 水平亦显著高于对照组(P<0.01);CC 基因型哮喘患儿Lyso-PC、apoB 水平明显高于CT和TT 基因型患儿(P<0.01)。结论 Lyso-PC 及apoB 可能干预哮喘发生的病理过程;血清中高Lyso-PC、apoB水平可能与哮喘易感基因ORMDL3 多态性rs12603332 位点共同作用导致儿童哮喘的发生。

Abstract

Objective To study the association of ORMDL3 single nucleotide polymorphisms (SNP) with lysophosphatidylcholine (LysoPC) and apolipoprotein B (apoB) levels. Methods A total of 300 children diagnosed with bronchial asthma between January 2010 and December 2012 were selected for the asthma group, and 298 children diagnosed with upper respiratory tract infection in the same period were selected for the control group. Serum LysoPC and apoB levels were measured using enzyme-linked immunosorbent assay. Genotype analysis was performed using the TaqMan probe. Results LysoPC and apoB levels were significantly higher in the asthma group than in the control group (P<0.01). Among children with various genotypes of ORMDL3 gene at locus rs12603332, the asthma group had significantly higher LysoPC and apoB levels than the control group (P<0.01). Among the children with asthma, those with CC genotype had significantly higher LysoPC and apoB levels than those with CT and TT genotypes (P<0.01). Conclusions LysoPC and apoB may intervene in the pathological process of asthma. Pro-inflammatory gene ORMDL3 SNP rs12603332 may be associated with high LysoPC and apoB levels, which leads to the occurrence of childhood asthma.

导出引用

王冰洁, 王桂兰, 陈德晖, 王文祥, 黄娟, 容嘉妍, 刘翔腾, 杨赛. 哮喘患儿ORMDL3基因多态性与溶血卵磷脂及载脂蛋白B的关系[J]. 中国当代儿科杂志. 2015, 17(3): 241-244 https://doi.org/10.7499/j.issn.1008-8830.2015.03.008

WANG Bing-Jie, WANG Gui-Lan, CHEN De-Hui, WANG Wen-Xiang, HUANG Juan, RONG Jia-Yan, LIU Xiang-Teng, YANG Sai. Association of ORMDL3 single nucleotide polymorphisms with lysophosphatidylcholine and apolipoprotein B levels in children with asthma[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(3): 241-244 https://doi.org/10.7499/j.issn.1008-8830.2015.03.008

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