Abstract：Objective To study the expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma, as well as their roles in the pathogenesis of asthma. Methods Thirty children with acute exacerbation of bronchial asthma were enrolled as the asthma group, and 20 healthy children were enrolled as the control group. RT-PCR and Western blot were used to measure the mRNA and protein expression levels of PI3K, NF-κB, and STAT1 in PBMCs. A spirometer was used to compare the pulmonary function between the two groups. The correlations between the mRNA expression of PI3K, NF-κB, and STAT1 and pulmonary function in children with bronchial asthma were analyzed. Results The asthma group had significantly higher mRNA and protein expression levels of PI3K, NF-κB, and STAT1 than the control group (P<0.05). Compared with the control group, the asthma group showed significant reductions in pulmonary function indices such as FEV1%, FEV1/FVC, and PEF% (P<0.05). In children with bronchial asthma, the mRNA expression levels of PI3K, NF-κB, and STAT1 were negatively correlated with FEV1%, FEV1/FVC, and PEF% (P<0.05). Conclusions The expression levels of PI3K, NF-κB, and STAT1 increase in children with asthma, and are negatively correlated with pulmonary function indices, suggesting that PI3K, NF-κB and STAT1 are involved in the development and progression of bronchial asthma in children.
SHI Hui-Ling,LIU Jie-Bo,LU Ai-Pin. Expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells in children with bronchial asthma[J]. CJCP, 2016, 18(7): 614-617.
Pacifico L, Osborn JF, Tromba V, et al. Helicobacter pylori infection and extragastric disorders in children: a critical update[J]. World J Gastroenterol, 2014, 20(6): 1379-1401.
[3]
Hasosah M, Satti M, Shehzad A, et al. Prevalence and risk factors of Helicobacter pylori infection in Saudi children: a three-year prospective controlled study[J]. Helicobacter, 2015, 20(1): 56-63.
[4]
Braga LL, Oliveira MA, Gonçalves MH, et al. CagA phosphorylation EPIYA-C motifs and the vacA i genotype in Helicobacter pylori strains of asymptomatic children from a high-risk gastric cancer area in northeastern Brazil[J]. Mem Inst Oswaldo Cruz, 2014, 109(8): 1045-1049.
[5]
Iwanczak B, Laszewicz W, Iwanczak F, et al. Genotypic and clinical differences of seropositive Helicobacter pylori children and adults in the Polish population[J]. J Physiol Pharmacol, 2014, 65(6): 801-807.
De Gusmão VR, Nogueira Mendes E, De Magalhães Queiroz DM, et al. vacA genotypes in Helicobacter pylori strains isolated from children with and without duodenal ulcer in Brazil[J]. J Clin Microbiol, 2000, 38(8): 2853-2857.
[8]
Yamaoka Y, Kodama T, Gutierrez O, et al. Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries[J]. J Clin Microbiol, 1999, 37(7): 2274-2279.
[9]
Ito Y, Azuma T, Ito S, et al. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan[J]. J Clin Microbiol, 1997, 35(7): 1710-1714.
[10]
Reyes-Leon A, Atherton JC, Argent RH, et al. Heterogeneity in the activity of Mexican Helicobacter pylori strains in gastric epithelial cells and its association with diversity in the cagA gene[J]. Infect Immun, 2007, 75(7): 3445-3454.
Shiota S, Suzuki R, Yamaoka Y. The significance of virulence factors in Helicobacter pylori[J]. J Dig Dis, 2013, 14(7): 341- 349.
[13]
Kim JY, Kim N, Nam RH, et al. Association of polymorphisms in virulence factor of Helicobacter pylori and gastroduodenal diseases in South Korea[J]. J Gastroenterol Hepatol, 2014, 29(5): 984-991.
[14]
Kolaylı F, Karadenizli A, Bingöl R, et al. Differences of vacA alleles and cagA gene positivity of Helicobacter pylori strains isolated from two different countries: Turkey and Germany[J]. Mikrobiyol Bul, 2012, 46(2): 332-334.
[15]
Mendoza-Elizalde S, Cortés-Márquez AC, Giono-Cerezo S, et al. Analysis of the genotypic diversity of strains of Helicobacter pylori isolated from pediatric patients in Mexico[J]. Infect Genet Evol, 2015, 29: 68-74.
Khan A, Farooqui A, Raza Y, et al. Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan[J]. J Infect Dev Ctries, 2013, 7(3): 220-228.
[18]
Biernat MM, Gościniak G, Iwańczak B. Prevalence of Helicobacter pylori cagA, vacA, iceA, babA2 genotypes in Polish children and adolescents with gastroduodenal disease[J]. Postepy Hig Med Dosw (Online), 2014, 68: 1015-1021.
[19]
Goncalves MH, Silva CI, Braga-Neto MB, et al. Helicobacter pylori virulence genes detected by string PCR in children from an urban community in northeastern Brazil[J]. J Clin Microbiol, 2013, 51(3): 988-989.
Ko JS, Kim KM, Oh YL, et al. cagA, vacA, and iceA genotypes of Helicobacter pylori in Korean children[J]. Pediatr Int, 2008, 50(5): 628-631.
[22]
Boyanova L, Yordanov D, Gergova G, et al. Association of iceA and babA genotypes in Helicobacter pylori strains with patient and strain characteristics[J]. Antonie Van Leeuwenhoek, 2010, 98(3): 343-350.
[23]
Ozbey G, Dogan Y, Demiroren K. Prevalence of Helicobacter pylori virulence genotypes among children in Eastern Turkey[J]. World J Gastroenterol, 2013, 19(39): 6585-6589.
[24]
Tanih NF, McMillan M, Naidoo N, et al. Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes in South African patients with upper gastrointestinal diseases[J]. Acta Trop, 2010, 116(1): 68-73.
[25]
Homan M, Šterbenc A, Kocjan BJ, et al. Prevalence of the Helicobacter pylori babA2 gene and correlation with the degree of gastritis in infected Slovenian children[J]. Antonie Van Leeuwenhoek, 2014, 106(4): 637-645.
[26]
Almeida N, Donato MM, Romãozinho JM, et al. Correlation of Helicobacter pylori genotypes with gastric histopathology in the central region of a South-European country[J]. Dig Dis Sc, 2015, 60(1): 74-85.
[27]
Vaziri F, Najar Peerayeh S, Alebouyeh M, et al. Diversity of Helicobacter pylori genotypes in Iranian patients with different gastroduodenal disorders[J]. World J Gastroenterol, 2013, 19(34): 5685-5692.
[28]
Sugimoto M, Zali MR, Yamaoka Y. The association of vacA genotypes and Helicobacter pylori-related gastroduodenal diseases in the Middle East[J]. Eur J Clin Microbiol Infect Dis, 2009, 28(10): 1227-1236.
[29]
Falsafi T, Khani A, Mahjoub F, et al. Analysis of vacA/cagA genotypes/status in Helicobacter pylori isolates from Iranian children and their association with clinical outcome[J]. Turk J Med Sci, 2015, 45(1): 170-177.
[30]
Homan M, Luzar B, Kocjan BJ, et al. Prevalence and clinical relevance of cagA, vacA, and iceA genotypes of Helicobacter pylori isolated from Slovenian children[J]. J Pediatr Gastroenterol Nutr, 2009, 49(3): 289-296.
[31]
Sedaghat H, Moniri R, Jamali R, et al. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA, babA2, and oipA genotypes in patients with upper gastrointestinal diseases[J]. Iran J Microbiol, 2014, 6(1): 14-21.
