Abstract：Objective To study the interactive regulatory effect of histone acetylation and methylation on cardiomyogenesis, and to provide a theoretical basis for the prevention and treatment of congenital heart disease. Methods A total of 24 Kunming mice were randomly divided into embryo day 14.5 (ED 14.5) group, embryo day 16.5 (ED 16.5) group, postnatal day 0.5 (PND 0.5) group, and postnatal day 7 (PND 7) group, with 6 mice in each group, and the heart tissue of fetal and neonatal mice was collected. Colorimetry was used to measure the activities of histone acetylases (HATs) and histone methyltransferases (HMTs) in the myocardium. Western blot was used to measure the expression of H3K9ac and H3K9me3 in the myocardium. Results Colorimetry showed that the activities of HATs and HMTs were higher before birth and were lower after birth. There was a significant difference in the activity of HATs in the myocardium between the PND 0.5 and PND 7 groups and the ED 14.5 group (P < 0.05), as well as between the PND 7 group and the ED 16.5 group (P < 0.05). There was also a significant difference in the activity of HMTs in the myocardium between the PND 7 group and the ED 14.5 and ED 16.5 groups (P < 0.05). Western blot showed higher expression of H3K9ac and H3K9me3 before birth and lower expression of H3K9ac and H3K9me3 after birth, and there were significant differences in the expression H3K9ac and H3K9me3 in the myocardium between the PND 0.5 and PND 7 groups and the ED 14.5 and ED 16.5 groups (P < 0.05). Conclusions The dynamic expression of HATs, HMTs, H3K9ac, and H3K9me3 is observed during cardiomyogenesis, suggesting that histone H3K9ac acetylation and histone H3K9me3 methylation mediated by HATs and HMTs may play a role in interactive regulation during cardiomyogenesis.
LI Shuo,LUO Xiao-Mei,PENG Bo-Hui et al. Interactive regulatory effect of histone H3K9ac acetylation and histone H3K9me3 methylation on cardiomyogenesis in mice[J]. CJCP, 2018, 20(11): 950-954.
Zhu H. Forkhead box transcription factors in embryonic heart development and congenital heart disease[J]. Life Sci, 2016, 144:194-201.
[2]
Dellborg M, Björk A, Pirouzi Fard MN, et al. High mortality and morbidity among adults with congenital heart disease and type 2 diabetes[J]. Scand Cardiovasc J, 2015, 49(6):344-350.
[3]
Freud LR, Tworetzky W. Fetal interventions for congenital heart disease[J]. Curr Opin Pediatr, 2016, 28(2):156-162.
[4]
Begic E, Begic Z, Jahic D, et al. Tetralogy of fallot-clinical course and treatment as a mirror of contemporary cardiology/cardiac surgery development in correction of congenital heart disease in the adults[J]. Med Arch, 2018, 72(3):224-226.
[5]
Madeira M, Ranchordas S, Oliveira P, et al. Pulmonary hypertension in valvular heart disease surgery:risk and prognosis[J]. Rev Port Cir Cardiotorac Vasc, 2017, 24(3-4):117.
[6]
Coppola A, Romito A, Borel C, et al. Cardiomyogenesis is controlled by the miR-99a/let-7c cluster and epigenetic modifications[J]. Stem Cell Res, 2014, 12(2):323-337.
[7]
Zhao W, Liu L, Pan B, et al. Epigenetic regulation of cardiac myofibril gene expression during heart development[J]. Cardiovasc Toxicol, 2015, 15(3):203-209.
Vallaster M, Vallaster CD, Wu SM. Epigenetic mechanisms in cardiac development and disease[J]. Acta Biochim Biophys Sin (Shanghai), 2012, 44(1):92-102.
[12]
Zhang QJ, Liu ZP. Histone methylations in heart development, congenital and adult heart diseases[J]. Epigenomics, 2015, 7(2):321-330.
[13]
Zhao W, Liu L, Pan B, et al. Epigenetic regulation of cardiac myofibril gene expression during heart development[J]. Cardiovasc Toxicol, 2015, 15(3):203-209.
[14]
Ooi JY, Tuano NK, Rafehi H, et al. HDAC inhibition attenuates cardiac hypertrophy by acetylation and deacetylation of target genes[J]. Epigenetics, 2015, 10(5):418-430.
[15]
Greco CM, Condorelli G. Epigenetic modifications and noncoding RNAs in cardiac hypertrophy and failure[J]. Nat Rev Cardiol, 2015, 12(8):488-497.
[16]
Zhang QJ, Liu ZP. Histone methylations in heart development, congenital and adult heart diseases[J]. Epigenomics, 2015, 7(2):321-330.
[17]
Chaturvedi P, Kalani A, Givvimani S, et al. Differential regulation of DNA methylation versus histone acetylation in cardiomyocytes during HHcy in vitro and in vivo:an epigenetic mechanism[J]. Physiol Genomics, 2014, 46(7):245-255.
