血小板表面TLR2和TLR4在儿童特发性血小板减少性紫癜中的变化及意义

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摘要目的：探讨血小板表面Toll样受体TLR2和TLR4、淋巴细胞表面CD86和血清中IL-2，IFN-γ，IL-4，IL-10表达的变化及相关性在儿童特发性血小板减少性紫癜（ITP）发病中的意义。方法：流式细胞学技术检测24例急性特发性血小板减少性紫癜(AITP)患儿、21例慢性特发性血小板减少性紫癜（CITP）患儿和20例对照组儿童血小板表面TLR2和TLR4，CD86的表达水平；ABC-ELISA法检测血清中IL-2，IL-4，IL-10和IFN-γ的浓度。结果：①AITP组、CITP组患儿CD41+TLR2+和CD61+TLR4+表达显著低于对照组(P<0.01)；且AITP组显著低于CITP组（P<0.01)；②AITP组和CITP组CD86+表达较对照组显著增高（P<0.01），AITP组和CITP组间差异无显著性；③AITP组和CITP组血清IL-2，IL-4，IL-10及IFN-γ浓度均较对照组显著增高（P＜0.05），AITP组和CITP组间差异无显著性。④相关性分析：CD41+TLR2+表达与CD61+TLR4+表达呈正相关，二者均与CD86+表达及血清IL-2，IL-4，IL-10浓度呈负相关，与血清IFN-γ浓度无相关性。结论：联合检测血小板表面TLR2和TLR4、淋巴细胞表面CD86的表达和血清中IL-2，IFN-γ，IL-4，IL-10含量的变化及相关性对于研究儿童ITP的发病机制以及早期分型具有重要参考价值。［中国当代儿科杂志，2009，11（10）：797-801］

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	王春美
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	邹湘
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关键词 ：特发性血小板减少性紫癜, Toll样受体, CD86, 细胞因子, 儿童

Abstract：OBJECTIVE: To study the changes and significance of Toll-like receptor-2 (TLR2) and Toll-like receptor-4 (TLR4) on platelets, CD86 on lymphocytes and concentrations of IL-2, IFN-γ, IL-4 and IL-10 in serum in children with idiopathic thrombocytopenic purpura (ITP). METHODS: Peripheral blood samples were collected from 24 children with acute idiopathic thrombocytopenic purpura (AITP), 21 children with chronic idiopathic thrombocytopenic purpura (CITP) and 20 normal children (control group). Expression of TLR2 and TLR4 on platelets and CD86 on lymphocytes were detected by flow cytometry. Serum concentrations of IL-2, IL-4, IL-10 and IFN-γ were measured using ABC-ELISA. RESULTS: The expression of CD41+TLR2+ and CD61+TLR4+ in the AITP and the CITP groups were significantly lower than those in the control group (P<0.01). The AITP group had lower expression of CD41+TLR2+ and CD61+TLR4+ than the CITP group (P<0.01). The expression of CD86+ in the AITP and the CITP groups was significantly higher than that in the control group (P<0.01). The serum concentrations of IL-2, IL-4, IL-10 and IFN-γ in the AITP and the CITP groups were significantly higher than those in the control group (P<0.05). There was a positive correlation between CD41+TLR2+ and CD61+TLR4+ expression. CD41+TLR2+ and CD61+TLR4+ expression were negatively correlated with CD86+ expression and serum concentrations of IL-2, IL-4 and IL-10. CONCLUSIONS: The detections of TLR2 and TLR4 on platelets, CD86 on lymphocytes and serum concentrations of IL-2, IFN-γ, IL-4 and IL-10 are of great value in understanding the pathogenesis and predicting types of ITP in children.［Chin J Contemp Pediatr, 2009, 11 (10):797-801］

Key words： Idiopathic thrombocytopenic purpura Toll-like receptor CD86 Cytokine Child

PACS:

R558+.2

引用本文:

王春美,盛光耀,邹湘等. 血小板表面TLR2和TLR4在儿童特发性血小板减少性紫癜中的变化及意义[J]. 中国当代儿科杂志, 2009, 11(10): 797-801.

Wang-Chun-Mei,CHENG Guang-Yao,JU Xiang et al. Levels of Toll-like receptors-2,-4 on platelets in children with idiopathic thrombocytopenic purpura[J]. CJCP, 2009, 11(10): 797-801.

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http://www.zgddek.com/CN/ 或 http://www.zgddek.com/CN/Y2009/V11/I10/797

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