CJCP
  中文版
  English Version
 
CJCP  2017, Vol. 19 Issue (6): 652-657    DOI: 10.7499/j.issn.1008-8830.2017.06.008
CLINICAL RESEARCH Current Issue| Next Issue| Archive| Adv Search  | 
NKX2.5 and TBX5 gene mutations in in vitro fertilization children with congenital heart disease
YANG Jing-Hui1,2, XU Xiao-Yan1,2, MI Hong-Ying1,2, JIANG Yan1,2, MA Xin-Mei1,2, LI Li1,2
Department of Pediatrics, First People's Hospital of Yunnan Province, Kunming 650032, China
Download: PDF (1502 KB)   HTML(114)
Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract  Objective To explore the differences of NKX2.5 and TBX5 gene mutations between in vitro fertilization (IVF) children with congenital heart disease (CHD) and naturally conceived children with CHD. Methods Blood samples from 68 IVF children with CHD and 98 naturally conceived children with CHD were collected. The mutations in coding regions 1 and 2 of the NKX2.5 gene, and coding regions 4, 5, and 8 of the TBX5 gene were examined by polymerase chain reaction (PCR) and DNA sequencing. Results An A-to-G mutation at nucleotide 63 (c.63A > G) in coding region 1 of the NKX2.5 gene was found in both IVF and naturally conceived children with CHD. There were no significant differences in genotype and allele frequencies at c.63A > G locus of the NKX2.5 gene between the two groups. No mutations were detected in coding region 2 of the NKX2.5 gene and coding regions 4, 5 and 8 of the TBX5 gene. Conclusions There is no difference in NKX2.5 and TBX5 gene mutations between IVF and naturally conceived children with CHD. Therefore, it is presumed that assisted reproductive technology may not lead to mutations in the NKX2.5 and TBX5 genes.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
YANG Jing-Hui
XU Xiao-Yan
MI Hong-Ying
JIANG Yan
MA Xin-Mei
LI Li
Key wordsCongenital heart disease      NKX2.5 gene      TBX5 gene      Gene mutation      Assisted reproductive technology      Child     
Received: 06 February 2017     
Cite this article:   
YANG Jing-Hui,XU Xiao-Yan,MI Hong-Ying et al. NKX2.5 and TBX5 gene mutations in in vitro fertilization children with congenital heart disease[J]. CJCP, 2017, 19(6): 652-657.
YANG Jing-Hui,XU Xiao-Yan,MI Hong-Ying et al. NKX2.5 and TBX5 gene mutations in in vitro fertilization children with congenital heart disease[J]. CJCP, 2017, 19(6): 652-657.
URL:  
http://www.zgddek.com/EN/10.7499/j.issn.1008-8830.2017.06.008     OR     http://www.zgddek.com/EN/Y2017/V19/I6/652
Copyright © 2004-2005 Chinese Journal Of Contemporary Pediatrics All rights reserved.
Addr: 87 Xiangya Road, Changsha, Hunan, 410008, PRC
Tel: 0731-84327402 Fax: 0731-84327922 E-mail: cjcp1999@csu.edu.cn