长链非编码RNA linc00467在儿童急性髓系白血病中的表达及耐药中的作用研究

饶春宝; 雒东; 林子添; 谢明玉; 胡媛; 彭琪; 江华; 张振洪; 陆小梅

doi:10.7499/j.issn.1008-8830.2002007

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中国当代儿科杂志 ›› 2020, Vol. 22 ›› Issue (7) : 734-738. DOI: 10.7499/j.issn.1008-8830.2002007

论著·临床研究

长链非编码RNA linc00467在儿童急性髓系白血病中的表达及耐药中的作用研究

饶春宝¹, 雒东¹, 林子添¹, 谢明玉², 胡媛², 彭琪², 江华³, 张振洪², 陆小梅^1,2

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Expression of long non-coding RNA linc00467 in childhood acute myeloid leukemia and its role in drug resistance

RAO Chun-Bao¹, LUO Dong¹, LIN Zi-Tian¹, XIE Ming-Yu², HU Yuan², PENG Qi², JIANG Hua³, ZHANG Zhen-Hong², LU Xiao-Mei^1,2

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摘要

目的研究长链非编码RNA linc00467在儿童急性髓系白血病（AML）中的表达及功能。方法采集2016年5月至2018年6月确诊的5例儿童AML病例的骨髓标本，以进行骨髓检查提示为正常的3例儿童骨髓标本作为对照。通过实时荧光定量PCR检测并比较linc00467在两组样本中的表达量。通过慢病毒系统在AML细胞（HL-60）中过表达linc00467（linc00467过表达组），以表达绿色荧光蛋白（GFP）的空载体转入AML细胞作为对照（GFP对照组）；通过慢病毒系统将干扰序列插入AML细胞中（sh-linc00467干扰组），以插入随机序列作为对照（sh-NC对照组）。检测各组细胞增殖情况及对阿霉素耐药性的影响。结果与对照组儿童相比，linc00467在儿童AML中表达上调。过表达与干扰linc00467表达对细胞增殖无明显影响（P > 0.05）。相比于GFP对照组，过表达linc00467在0.1、0.2、0.3、0.4、0.5 μg/mL阿霉素浓度作用下均可增强HL-60细胞活性（P < 0.05）。相比于sh-NC对照组，干扰linc00467的表达在0.1、0.2、0.3、0.4、0.5 μg/mL阿霉素浓度作用下均可降低HL-60细胞活性（P < 0.05）。与未处理组相比，阿霉素处理HL-60细胞后linc00467表达量升高（P < 0.05）。结论 linc00467有促进AML细胞对阿霉素耐药的生物学功能，为AML新的治疗药物开发提供了依据。

Abstract

Objective To study the expression and function of long non-coding RNA linc00467 in childhood acute myeloid leukemia (AML). Methods Bone marrow samples were collected from 5 children with AML who were diagnosed from May 2016 to June 2018. Normal bone marrow samples based on bone marrow examination were collected from 3 children as controls. Quantitative real-time PCR was used to measure the expression of linc00467 in the two groups. A lentivirus system was used to achieve overexpression of linc00467 in AML cells (HL-60) (linc00467 overexpression group), and empty vector expressing green fluorescent protein (GFP) was transfected into AML cells to establish a GFP control group. A lentivirus system was used to insert an interfering sequence into AML cells (sh-linc00467 interfering group), and a random sequence was inserted to establish an sh-NC control group. Cell proliferation and resistance to doxorubicin were observed for all groups. Results Compared with the normal control group, the children with AML had a significant increase in linc00467 (P=0.018). Overexpression and interference with linc00467 expression had no significant effect on cell proliferation. Compared with the GFP control group, the linc00467 overexpression group had a significant increase in the viability of HL-60 cells at the adriamycin concentrations of 0.1, 0.2, 0.3, 0.4, and 0.5 μg/mL (P < 0.05). Compared with the sh-NC control group, the sh-linc00467 interfering group had a significant reduction in the viability of HL-60 cells at the adriamycin concentrations of 0.1, 0.2, 0.3, 0.4, and 0.5 μg/mL (P < 0.05). Compared with the untreated group, the adriamycin treatment group had a significant increase in the expression of linc00467 in HL-60 cells (P < 0.05). Conclusions This study reveals the biological function of linc00467 to promote the resistance to adriamycin in AML, which provides a basis for developing new therapeutic drugs for AML.

导出引用

饶春宝, 雒东, 林子添, 谢明玉, 胡媛, 彭琪, 江华, 张振洪, 陆小梅. 长链非编码RNA linc00467在儿童急性髓系白血病中的表达及耐药中的作用研究[J]. 中国当代儿科杂志. 2020, 22(7): 734-738 https://doi.org/10.7499/j.issn.1008-8830.2002007

RAO Chun-Bao, LUO Dong, LIN Zi-Tian, XIE Ming-Yu, HU Yuan, PENG Qi, JIANG Hua, ZHANG Zhen-Hong, LU Xiao-Mei. Expression of long non-coding RNA linc00467 in childhood acute myeloid leukemia and its role in drug resistance[J]. Chinese Journal of Contemporary Pediatrics. 2020, 22(7): 734-738 https://doi.org/10.7499/j.issn.1008-8830.2002007

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