0~6岁儿童淋巴细胞免疫分型影响因素探讨

陈睿; 张修发; 周天祥; 陈滢; 钟文明; 马丽亚

doi:10.7499/j.issn.1008-8830.2014.11.008

PDF(1296 KB)

HTML

中国当代儿科杂志 ›› 2014, Vol. 16 ›› Issue (11) : 1114-1117. DOI: 10.7499/j.issn.1008-8830.2014.11.008

论著·临床研究

0~6岁儿童淋巴细胞免疫分型影响因素探讨

陈睿¹, 张修发², 周天祥², 陈滢³, 钟文明⁴, 马丽亚¹

作者信息 +

Influencing factors for lymphocyte subsets in children aged 0-6 years

CHEN Rui¹, ZHANG Xiu-Fa², ZHOU Tian-Xiang², CHEN Ying³, ZHONG Wen-Ming⁴, MA Li-Ya¹

Author information +

文章历史 +

摘要

目的探讨影响0~6 岁儿童淋巴细胞免疫分型的因素.方法采集45 例健康足月儿脐动脉血及79 例健康儿童静脉血,按不同生产方式将45 例足月儿分为顺产组(n=22)和剖宫产组(n=23);按不同年龄段将健康儿童分为28 d~ 组(n=25)、1 岁~ 组(n=26)和3~6 岁组(n=28),用流式细胞术检测淋巴细胞亚群值,分析生产方式、性别和年龄对淋巴细胞免疫分型的影响.结果顺产和剖宫产两组新生儿在T 和Ts 细胞计数、B 细胞百分比、NK 细胞百分比和绝对计数等指标上差异均有统计学意义(均P<0.05);各淋巴细胞免疫表型绝对计数和百分比在不同性别健康儿童之间比较差异均无统计学意义(均P>0.05);除Ts 和NK 细胞计数外,其余各亚群在不同年龄组儿童之间差异均有统计学意义(均P<0.05).结论儿童淋巴细胞亚群的分布受年龄因素的影响较大,新生儿出生时也受生产方式的影响;在建立儿童淋巴细胞免疫分型正常参考范围时应按年龄分段.

Abstract

Objective To investigate the influencing factors for lymphocyte subsets in children 0 to 6 years of age.Methods Umbilical artery blood samples from 45 healthy full-term infants and venous blood samples from 79 healthychildren between 0 and 6 years were collected. According to the methods of delivery, the full-term infants were dividedinto vaginal delivery group (n=22) and cesarean section group (n=23). Healthy children were divided into different agegroups:28 days to 12 months (n=25), 1-3 years (n=26), and 3-6 years (n=28). Lymphocyte subsets were examined byflow cytometry. The influencing factors including delivery method, sex, and age, which might have an effect on the lymphocyte subsets, were analyzed. Results There were significant differences in T and Ts cell counts, percentage of Bcells, and percentage and count of natural killer (NK) cells between the full-term infants of vaginal delivery and cesareansection (P<0.05). The absolute counts and percentages of different lymphocyte subsets showed no significant differences between males and females in healthy children (P>0.05). The counts of all lymphocyte subsets except Ts and NK cellsvaried significantly between different age groups (P<0.05). Conclusions Lymphocyte subsets in children under 6years of age are more profoundly affected by age. Delivery method is also a contributing factor in newborn infants. Thereference range of lymphocyte subsets in children should be established for different age groups.

导出引用

陈睿, 张修发, 周天祥, 陈滢, 钟文明, 马丽亚. 0~6岁儿童淋巴细胞免疫分型影响因素探讨[J]. 中国当代儿科杂志. 2014, 16(11): 1114-1117 https://doi.org/10.7499/j.issn.1008-8830.2014.11.008

CHEN Rui, ZHANG Xiu-Fa, ZHOU Tian-Xiang, CHEN Ying, ZHONG Wen-Ming, MA Li-Ya. Influencing factors for lymphocyte subsets in children aged 0-6 years[J]. Chinese Journal of Contemporary Pediatrics. 2014, 16(11): 1114-1117 https://doi.org/10.7499/j.issn.1008-8830.2014.11.008

参考文献

[1] Schatorje EJ, Gemen EF, Driessen GJ, et al. Paediatric reference values for the peripheral T cell compartment[J]. Scand J Immunol, 2012, 75(4):436-444.
[2] 刘义红, 熊丽萍, 沈红元. 毛细支气管炎患儿红细胞免疫及 T 细胞亚群变化的研究[J]. 中国当代儿科杂志, 2011, 13(3):202-204.
[3] 康妍萌, 丁明杰, 韩玉玲, 等. 重症肺炎支原体肺炎患儿肺泡灌洗液中Th1/Th2 细胞免疫应答状况的研究[J]. 中国当代儿科杂志, 2011, 13(3):188-190.
[4] 袁远宏, 肖政辉, 张慧, 等. 小儿脓毒症T 细胞亚群变化分析及临床意义[J]. 中国当代儿科杂志, 2013, 15(3):216-218.
[5] Huenecke S, Behl M, Fadler C, et al. Age-matched lymphocyte subpopulation reference values in childhood and adolescence:application of exponential regression analysis[J]. Eur J Haematol, 2008, 80(6):532-539.
[6] Garcia-Dabrio MC, Pujol-Moix N, Martinez-Perez A, et al. Influence of age, gender and lifestyle in lymphocyte subsets:report from the Spanish Gait-2 Study[J]. Acta Haematol, 2012, 127(4):244-249.
[7] van Gent R, van Tilburg CM, Nibbelke EE, et al. Refined characterization and reference values of the pediatric T-and B-cell compartments[J]. Clin Immunol, 2009, 133(1):95-107.
[8] 班开斌, 赵艳丽, 黄友敏, 等. 不同分娩方式对脐血抵抗素及新生儿免疫功能的影响[J]. 医学检验与临床, 2008, 19(5):18-20.
[9] 刘克芹, 刘晨, 尹卫东, 等. 不同分娩方式不同性别新生儿脐血的免疫功能检测[J]. 中国组织工程研究, 2012, 16(27):5092-5096.
[10] Perez A, Gurbindo MD, Resino S, et al. NK cell increase in neonates from the preterm to the full-term period of gestation[J]. Neonatology, 2007, 92(3):158-163.
[11] Sagnia B, Ateba Ndongo F, Ndiang Moyo Tetang S, et al. Reference values of lymphocyte subsets in healthy, HIVnegative children in Cameroon[J]. Clin Vaccine Immunol, 2011, 18(5):790-795.
[12] Fuentes-Arderiu X, Mestre M. Description of flow cytometry examinations related to human cell differentiation molecules in clinical immunology[J]. Cytometry B Clin Cytom, 2009, 76(4):291-293.
[13] Schatorje EJ, Gemen EF, Driessen GJ, et al. Paediatric reference values for the peripheral T cell compartment[J]. Scand J Immunol, 2012, 75(4):436-444.
[14] 肖婧, 申丹, 刘永革, 等. 以流式细胞术建立中国汉族健康儿童外周血淋巴细胞亚群相对计数的正常参考值[J]. 中国循证儿科杂志, 2010, 5(4):245-250.
[15] Ashman M, Sachdeva N, Davila L, et al. Influence of 4-and 6-color flow cytometers and acquisition/analysis softwares on the determination of lymphocyte subsets in HIV infection[J]. Cytometry B Clin Cytom, 2007, 72(5):380-386.