IL-17A基因多态性与儿童哮喘易感性的相关性研究

钟芳芳; 邹艳; 刘春艳; 刘文君

doi:10.7499/j.issn.1008-8830.2016.12.013

PDF(1418 KB)

HTML

中国当代儿科杂志 ›› 2016, Vol. 18 ›› Issue (12) : 1264-1268. DOI: 10.7499/j.issn.1008-8830.2016.12.013

论著·临床研究

IL-17A基因多态性与儿童哮喘易感性的相关性研究

钟芳芳, 邹艳, 刘春艳, 刘文君

作者信息 +

Relationship between interleukin-17A gene polymorphisms and the susceptibility to childhood asthma

ZHONG Fang-Fang, ZOU Yan, LIU Chun-Yan, LIU Wen-Jun

Author information +

文章历史 +

摘要

目的探讨儿童IL-17A启动子区域（-197G/A和-692C/T）基因多态性与儿童哮喘易感性的关系，为能进一步寻找到哮喘的候选基因从而为患病高风险儿童早期预防奠定基础。方法选取2013年8月至2015年8月门诊随访或住院的哮喘患儿65例为哮喘组，另选取同期行健康体检儿童70例为健康对照组，采集两组儿童外周静脉血，应用序列特异性引物聚合酶链反应（SSP-PCR）法检测IL-17A基因-197G/A和-692C/T两个位点的单核苷酸多态性（SNP），统计分析两组间基因型及等位基因分布频率的差异。结果 IL-17A基因-692C/T位点哮喘组患儿TT基因型的分布频率（29%）显著高于健康对照组（16%）（P=0.012）；哮喘组-692T等位基因分布频率（52%）显著高于健康对照组（42%）（P=0.039）；罹患儿童哮喘的风险T等位基因携带者是C等位基因携带者的1.413倍（OR=1.413，95%CI：1.015~1.917）；而IL-17A基因-197G/A位点基因型及等位基因分布频率在哮喘组和健康对照组间比较差异无统计学意义（P > 0.05）。结论 IL-17A基因启动子区域-692C/T位点基因多态性与儿童哮喘的易感性相关，-692T等位基因携带者更易罹患儿童哮喘，而IL-17A-197G/A位点多态性与儿童哮喘的易感性无显著相关。

Abstract

Objective To explore the relationship between polymorphisms of interleukin-17A (IL-17A) gene promoter (-197G/A and -692C/T) and the susceptibility to childhood asthma, to further identify the candidate genes for asthma, and to provide a basis for early prevention of asthma in high-risk children. Methods Sixty-five outpatients or inpatients with childhood asthma between August 2013 and August 2015 were assigned to asthma group. Seventy healthy children within the same period were assigned to control group. Using peripheral venous blood from the two groups, PCR with sequence-specific primers was carried out to determine single nucleotide polymorphisms at positions -197G/A and -692C/T in IL-17A gene promoter. A statistical analysis was used to evaluate differences in genotype and allele frequencies between the two groups. Results Compared with the control group, the asthma group had significantly higher frequencies of TT genotype (29% vs 16%;P=0.012) and T allele (52% vs 42%;P=0.039) at position -692C/T of IL-17A gene. Children with T allele had 1.413-fold higher risk of childhood asthma than those with C allele (OR=1.413, 95%CI:1.015-1.917). There were no significant differences in genotype and allele frequencies at position -197G/A in IL-17A gene between the two groups (P > 0.05). Conclusions Polymorphisms at position -692C/T in IL-17A gene promoter is associated with the susceptibility to childhood asthma. Children with -692T allele are more susceptible to childhood asthma. There is no significant relationship between polymorphisms at position -197G/A in IL-17A gene promoter and the susceptibility to childhood asthma.

导出引用

钟芳芳, 邹艳, 刘春艳, 刘文君. IL-17A基因多态性与儿童哮喘易感性的相关性研究[J]. 中国当代儿科杂志. 2016, 18(12): 1264-1268 https://doi.org/10.7499/j.issn.1008-8830.2016.12.013

ZHONG Fang-Fang, ZOU Yan, LIU Chun-Yan, LIU Wen-Jun. Relationship between interleukin-17A gene polymorphisms and the susceptibility to childhood asthma[J]. Chinese Journal of Contemporary Pediatrics. 2016, 18(12): 1264-1268 https://doi.org/10.7499/j.issn.1008-8830.2016.12.013

参考文献

[1] 吴红梅. 血清IL-17在支气管哮喘患儿中的表达意义[J]. 中国医药指南, 2014, 12(23):49-50.
[2] Li J, Tian H, Jiang HJ, et al. Interleukin-17 SNPs and serum levels increase ulcerative colitis risk:a meta-analysis[J]. World J Gastroenterol, 2014, 20(42):15899-15909.
[3] 陈育智. 支气管哮喘[M]//胡亚美, 江载芳. 诸福棠实用儿科学. 第7版. 北京:人民卫生出版社, 2002:635.
[4] 宋妙丽, 杨晓春, 申咏梅, 等. PCR-SSP法和基因测序检测人胰腺癌细胞株Patu 8988 K-ras基因第12位密码子点突变及其方式[J]. 苏州大学学报(医学版), 2008, 28(4):545-548.
[5] 朱春燕, 聂咏梅, 周豪杰. PCR-SSP法检测HPA-15基因频率的可靠性分析[J]. 热带医学杂志, 2015, 15(7):897-898.
[6] Losappio L, Heffier E, Contento F, et al. Thunderstorm-related asthma epidemic owing to Olea Europaea pollen sensitization[J]. Allergy, 2011, 66(11):1510-1511.
[7] Kuschner WG. The asthma epidemic[J]. N Engl J Med, 2007, 356(10):1073.
[8] Shafazand S, Colice G. Asthma:the epidemic has ended, or has it?[J]. Chest, 2004, 125(6):1969-1970.
[9] Chen ZH, Wang PL, Shen HH. Asthma research in China:a five-year review[J]. Respirology, 2013, 18 Suppl 3:10-19.
[10] 全国儿科哮喘协作组, 中国疾病预防控制中心环境与健康相关产品安全所. 第三次中国城市儿童哮喘流行病学调查[J]. 中华儿科杂志, 2013, 51(10):729-735.
[11] Akdis CA, Akdis M, Bieber T, et al. Diagnosis and treatment of atopic dermatitis in children and adults:European Academy of Allergology and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL Consensus Report[J]. J Allergy Clin Immunol, 2006, 118(1):152-169.
[12] Vogt H, Bråbäck L, Zetterström O, et al. Asthma heredity, cord blood IgE and asthma-related symptoms and medication in adulthood:a long-term follow-up in a Swedish birth cohort[J]. PLoS One, 2013, 8(6):e66777.
[13] Zhou H, Hong X, Jiang S, et al. Analyses of associations between three positionally cloned asthma candidate genes and asthma or asthma-related phenotypes in a Chinese population[J]. BMC Med Genet, 2009, 10:123.
[14] Li X, Wills-Karp M, Ewart S. Investigating Gata3 as a positional candidate gene for allergic asthma in a murine model[J]. Int J Immunogenet, 2006, 33(5):333-337.
[15] Meyer DM, Jesson MI, Li X, et al. Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690, 550, in rat adjuvant-induced arthritis[J]. J Inflamm (Lond), 2010, 7:41.
[16] Wang H, Zhang Y, Liu Z, et al. The IL-17A G-197A and IL-17F 7488T/C polymorphisms are associated with increased risk of cancer in Asians:a meta-analysis[J]. Drug Des Devel Ther, 2015, 9:5159-5168.
[17] Long ZW, Yu HM, Wang YN, et al. Association of IL-17 polymorphisms with gastric cancer risk in Asian populations[J]. World J Gastroenterol, 2015, 21(18):5707-5718.
[18] Maalmi H, Beraies A, Charad R, et al. IL-17A and IL-17F genes variants and susceptibility to childhood asthma in Tunisia[J]. J Asthma, 2014, 51(4):348-354.
[19] 王娟, 周娟, 蔺丽慧, 等. IL-17基因启动子区单核苷酸多态性与儿童哮喘的关系[J]. 第二军医大学学报, 2011, 32(5):481-484.
[20] Vargas-Alarcón G, Angeles-Martínez J, Villarreal-Molina T, et al. Interleukin-17A gene haplotypes are associated with risk of premature coronary artery disease in Mexican patients from the Genetics of Atherosclerotic Disease (GEA) study[J]. PLoS One, 2015, 10(1):e0114943.