目的：研究CD4+ CD25+ Foxp3+ 调节性T细胞（Treg细胞）在癫癎患儿外周血的表达水平，探讨其在癫癎发病机制中的作用。方法：采用流式细胞术分别检测41例癫癎患儿和38例健康儿童外周血Treg细胞占CD4+ T细胞的比例，及CD4+ T细胞、CD8+ T细胞、自然杀伤细胞（NK）、B细胞分别占淋巴细胞的比例。结果：癫癎组患儿外周血Treg细胞比例、CD4+ T细胞比例、CD4+ /CD8+ 比值分别为（2.4±0.5）%、（35±5）%、1.32±0.24，较对照组的（6.1±1.2）%、（38±4）%、1.60±0.24显著降低（P<0.05）；而癫癎组CD8+ T细胞比例、NK细胞比例、B淋巴细胞比例分别为（27±3）%、（11.1±5.1）%、（24±9）%，较对照组的（24±3）%、（8.5±1.9）%、(16±5)%显著升高（P<0.05）。结论：癫癎患儿外周血中存在 Treg 细胞比例异常，提示免疫机制可能参与了癫癎的发生和发展。
Abstract
OBJECTIVE: To study the levels of CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) in the peripheral blood of children with epilepsy and the roles of Tregs in the pathogenesis of epilepsy. METHODS: Forty-one children with epilepsy and thirty-eight healthy children were enrolled. The percentage of CD4+ CD25+ Foxp3+ Tregs in CD4+ T cells and the percentages of CD4+ T cells, CD8+ T cells, nature killer (NK) cells and B cells in lymphocytes were evaluated by flow cytometry. RESULTS: The percentages of peripheral blood CD4+ CD25+ Foxp3+ Tregs and CD4+ T cells and the ratio of CD4+ T cells to CD8+ T cells in epileptic children were (2.4±0.5)%, (35±5)% and 1.32±0.24 respectively, which were significantly lower than those in healthy children ［(6.1±1.2)%, (38±4)% and 1.60±0.24 respectively; P<0.05］. In contrast, the percentages of CD8+ T cells, NK cells and B cells in lymphocytes in epileptic children were significantly higher than those in healthy children［(27±3)% vs (24±3)%, (11.1±5.1)% vs (8.5±1.9)% and (24±9)% vs (16±5)% respectively; P<0.05］. CONCLUSIONS: The abnormal percentage of CD4+ CD25+ Foxp3+ Tregs in the peripheral blood may be involved in the pathogenesis of epilepsy.
关键词
癫癎 /
CD4+ CD25+ Foxp3+ /
调节性T细胞 /
流式细胞术 /
儿童
Key words
Epilepsy /
CD4+ CD25+ Foxp3+ /
Regulatory T cell /
Flow cytometry /
Child
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参考文献
［1］Marson A, Kretschmer K, Frampton GM, Jacobsen ES, Polansky JK, MacIsaac KD, et al. Foxp3 occupancy and regulation of key target genes during T-cell stimulation［J］. Nature, 2007, 445(7130): 931-935.
［2］Lehtim-ki KA, Ker-men T, Palmio J, M-kinen R, Hurme M, Honkaniemi J, et al. Increased plasma levels of cytokines after seizures in localization-related epilepsy［J］．Acta Neurol Scand, 2007, 116(4)：226-230.
［3］Sakaguchi S. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and nonself［J］. Nat Immunol, 2005, 6（4）: 345-352.
［4］Engel J. International League Against Epilepsy (ILAE). A proposed diagnostic scheme for people with epileptic seizures and with epilepsy: report of the ILAE Task Force on Classification and Terminology［J］. Epilepsia, 2001, 42(6): 796-803.
［5］EI Andaloussi A, Lesniak MS. An increase in CD4+CD25+Foxp3+ regulatory T cells in tumor-infiltrating lymphocytes of human glioblastoma multiforme［J］. Neuro Oncol, 2006, 8(3): 234-243.
［6］Bauer S, K-ller M, Cepok S, Todorova-Rudolph A, Nowak M, Nockher WA, et al. NK and CD4+ T cell changes in blood after seizures in temporal lobe epilepsy［J］. Exp Neurol, 2008, 211(2): 370-377.
［7］Montelli TC, Soares AM, Pera-oli MT. Immunologic aspects of West syndrome and evidence of plasma inhibitory effects on T cell function［J］. Arq Europsiquiatr, 2003, 61(3B): 731-737.
［8］蓝菊红，吴日荷，吕卓超.热性惊厥患儿CD4+、CD8+、CD4+/CD8+的变化及意义［J］.中国当代儿科杂志，2009, 11(6):492-493.
［9］Bhatt R, Bhatt S, Hameed M, Rameshwar P, Siegel A. Amygdaloid kindled seizures can induce functional and pathological changes in thymus of rat: role of the sympathetic nervous system［J］. Neurobiol Dis, 2006, 21（1）：127-137.
［10］陈英才，穆莉芳，梁淑芬，吴春燕，赵红立，谢丹.儿童癫癎的细胞免疫研究［J］. 中国当代儿科杂志，2005, 7(4):374-375.
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