目的：探讨血清S100B蛋白在早产儿脑损伤中的变化及意义。方法：47名早产儿纳入本研究，根据影像学MRI和头颅B超检查结果分为无脑损伤组（20 例）、非脑白质损伤组（14例）和脑白质损伤组（13例）。留取生后＜24 h、72 h及7 d的血清样本，采用酶联免疫吸附测定法（ELISA）双抗体夹心法测定S100B蛋白水平。结果：脑白质损伤组和非脑白质损伤组出生后24 h、72 h及7 d血清S100B蛋白水平均明显高于无脑损伤组（P＜0.05），且脑白质损伤组血清S100B蛋白水平在各监测时间点明显高于非脑白质损伤组（P＜0.05）。结论：脑损伤早产儿生后7 d内血清S100B蛋白水平增高，提示血清S100B蛋白可以作为一种脑损伤的早期敏感标志物，对于临床早产儿脑损伤特别是脑白质损伤的诊断更有意义。
Abstract
OBJECTIVE: To study changes of serum S100B protein level in preterm infants with brain damage and its role. METHODS: Forty-seven preterm infants were classified into 3 groups based on the results of brain ultrasound and MRI: brain white matter damage (WMD; n=13), brain but not white matter damage (non-WMD; n=14) and control (no brain damage; n=20). Blood samples were collected within 24 hrs, 72 hrs and 7 days after birth. S100B protein level was measured using ELISA. RESULTS: Serum levels of S100B in the WMD and non-WMD groups were significantly higher than in the control group within 24 hours, 72 hours and 7 days after birth (P<0.05). More increased serum S100B levels were observed in the WMD group compared with the non-WMD group (P<0.05). CONCLUSIONS: Serum S100B protein level increases in preterm infants with brain damage within 7 days after birth, suggesting that it may be used as an early sensitive marker for the diagnosis of brain damage, especially WMD.
关键词
血清S100B /
脑白质损伤 /
早产儿
Key words
S100B protein /
Brain white matter damage /
Preterm infant
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］Paneth N, Rudelli R, Kazam E, Monte W. Brain damage in preterm infant［M］. London:Mac Keith Press, 1994: 99-118.
［2］Argyropoulou MI. Brain lesions in preterm infants: initial diagnosis and followup［J］. Pediatr Radiol, 2010, 40(6): 811-818.
［3］Neil JJ, Inder TE. Imaging perinatal brain injury in premature infants［J］. Semin Perinatol, 2004, 28(6): 433-443.
［4］周丛乐.影像学检查在早产儿脑损伤诊断中意义［J］.临床儿科杂志，2006，24（3）：170-172.
［5］Debillon T, N′Guyen S, Muet A, Quere MP, Moussaly F, Roze JC. Limitations of ultrasonography for diagnosing white matter damage in preterm infants［J］. Arch Dis Child Fetal Neonatal Ed, 2003, 88(4): F275-F279.
［6］Schafer BW, Heizmann CW. The S100 family of EF-hand calcium-binding protein: functions and pathology［J］. Trends Biochem Sci, 1996, 21(4): 134-140.
［7］Nagdyman N, Grimmer I, Scholz T, Muller C, Obladen M. Predictive value of brain-specific proteins in serum for neurodevelopmental outcome after birth asphyxia［J］. Pediatr Res, 2003, 54(2): 270-275.
［8］王庆红，宋健辉，王霞，刘沉涛，毕丹东.血清S100B蛋白在新生儿窒息后脑损伤中的临床意义［J］. 中国当代儿科杂志，2005，7（4）：318-320.
［9］朱阿瑾，余增渊，李天慧，方霞.血清S100B蛋白在缺氧缺血性脑病新生儿脑损伤中的诊断价值［J］.实用临床儿科杂志，2010，25（11）：853-855.
PDF(1302 KB)
