长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用

赵凤艳; 唐军; 张莉; 李世平; 冯艺; 刘海婷; 屈艺; 母得志

doi:10.7499/j.issn.1008-8830.2015.12.018

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中国当代儿科杂志 ›› 2015, Vol. 17 ›› Issue (12) : 1348-1353. DOI: 10.7499/j.issn.1008-8830.2015.12.018

论著·实验研究

长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用

赵凤艳, 唐军, 张莉, 李世平, 冯艺, 刘海婷, 屈艺, 母得志

作者信息 +

Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells

ZHAO Feng-Yan, TANG Jun, ZHANG Li, LI Shi-Ping, FENG Yi, LIU Hai-Ting, QU Yi, MU De-Zhi

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文章历史 +

摘要

目的探讨长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用。方法将大鼠肾上腺嗜铬细胞瘤(PC12)细胞分为常氧组、氧糖剥夺处理组(OGD组)、小分子RNA常氧组(siRNA组)及siRNA OGD组, n=3。以无葡萄糖无血清DMEM培养液于37℃、1%O2+99% N₂/CO₂条件下培养细胞6 h,建立缺氧缺血体外模型。采用实时定量PCR法检测BC088414、肾上腺素受体β2(Adrb2)和半胱氨酸蛋白酶6(Casp6) mRNA的表达;利用小分子干扰RNA(siRNA)抑制PC12细胞中BC088414的表达;采用TUNEL法检测细胞凋亡情况。结果 OGD组细胞凋亡指数明显高于常氧组(P<0.01),抑制BC088414表达后,PC12细胞的凋亡指数明显低于OGD组(P<0.05)。经OGD处理后,PC12细胞中BC088414、Adrb2和Casp6 mRNA表达水平较常氧组均明显增加(P<0.05);抑制BC088414表达,可导致PC12细胞中Adrb2和Casp6 mRNA表达水平较常氧组降低,且可抑制OGD诱导的Adrb2和Casp6 mRNA表达上调(P<0.05)。结论长链非编码RNABC088414可能通过Adrb2和Casp6促进凋亡,加重缺氧缺血引起的神经细胞损伤。

Abstract

Objective To investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells. Methods Rat adrenal pheochromocytoma (PC12) cells were divided into four groups:normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure mRNA expression of lncRNA BC088414, β2-adrenoceptor (Adrb2), and caspase-6(CASP6). siRNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis. Results The OGD group had a significantly higher cell apoptotic index than the normoxic group (P<0.01). After inhibition of BC088414 expression, the OGD group had a significantly reduced apoptotic index (P<0.05). The OGD group had significantly higher mRNA expression levels of lncRNA BC088414, Adrb2, and CASP6 compared with the normoxic group (P<0.05). The siRNA -normoxic group had significantly lower mRNA expression levels of Adrb2 and CASP6 than the normoxic group (P<0.05), and the siRNA-OGD group also had significantly lower mRNA expression levels of Adrb2 and CASP6 than the OGD group (P<0.05). Conclusions LncRNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.

导出引用

赵凤艳, 唐军, 张莉, 李世平, 冯艺, 刘海婷, 屈艺, 母得志. 长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用[J]. 中国当代儿科杂志. 2015, 17(12): 1348-1353 https://doi.org/10.7499/j.issn.1008-8830.2015.12.018

ZHAO Feng-Yan, TANG Jun, ZHANG Li, LI Shi-Ping, FENG Yi, LIU Hai-Ting, QU Yi, MU De-Zhi. Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells[J]. Chinese Journal of Contemporary Pediatrics. 2015, 17(12): 1348-1353 https://doi.org/10.7499/j.issn.1008-8830.2015.12.018

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