Abstract：Objective To explore the relationship between polymorphisms of interleukin-17A (IL-17A) gene promoter (-197G/A and -692C/T) and the susceptibility to childhood asthma, to further identify the candidate genes for asthma, and to provide a basis for early prevention of asthma in high-risk children. Methods Sixty-five outpatients or inpatients with childhood asthma between August 2013 and August 2015 were assigned to asthma group. Seventy healthy children within the same period were assigned to control group. Using peripheral venous blood from the two groups, PCR with sequence-specific primers was carried out to determine single nucleotide polymorphisms at positions -197G/A and -692C/T in IL-17A gene promoter. A statistical analysis was used to evaluate differences in genotype and allele frequencies between the two groups. Results Compared with the control group, the asthma group had significantly higher frequencies of TT genotype (29% vs 16%;P=0.012) and T allele (52% vs 42%;P=0.039) at position -692C/T of IL-17A gene. Children with T allele had 1.413-fold higher risk of childhood asthma than those with C allele (OR=1.413, 95%CI:1.015-1.917). There were no significant differences in genotype and allele frequencies at position -197G/A in IL-17A gene between the two groups (P > 0.05). Conclusions Polymorphisms at position -692C/T in IL-17A gene promoter is associated with the susceptibility to childhood asthma. Children with -692T allele are more susceptible to childhood asthma. There is no significant relationship between polymorphisms at position -197G/A in IL-17A gene promoter and the susceptibility to childhood asthma.
ZHONG Fang-Fang,ZOU Yan,LIU Chun-Yan et al. Relationship between interleukin-17A gene polymorphisms and the susceptibility to childhood asthma[J]. CJCP, 2016, 18(12): 1264-1268.
Akdis CA, Akdis M, Bieber T, et al. Diagnosis and treatment of atopic dermatitis in children and adults:European Academy of Allergology and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL Consensus Report[J]. J Allergy Clin Immunol, 2006, 118(1):152-169.
[12]
Vogt H, Bråbäck L, Zetterström O, et al. Asthma heredity, cord blood IgE and asthma-related symptoms and medication in adulthood:a long-term follow-up in a Swedish birth cohort[J]. PLoS One, 2013, 8(6):e66777.
[13]
Zhou H, Hong X, Jiang S, et al. Analyses of associations between three positionally cloned asthma candidate genes and asthma or asthma-related phenotypes in a Chinese population[J]. BMC Med Genet, 2009, 10:123.
[14]
Li X, Wills-Karp M, Ewart S. Investigating Gata3 as a positional candidate gene for allergic asthma in a murine model[J]. Int J Immunogenet, 2006, 33(5):333-337.
[15]
Meyer DM, Jesson MI, Li X, et al. Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690, 550, in rat adjuvant-induced arthritis[J]. J Inflamm (Lond), 2010, 7:41.
[16]
Wang H, Zhang Y, Liu Z, et al. The IL-17A G-197A and IL-17F 7488T/C polymorphisms are associated with increased risk of cancer in Asians:a meta-analysis[J]. Drug Des Devel Ther, 2015, 9:5159-5168.
[17]
Long ZW, Yu HM, Wang YN, et al. Association of IL-17 polymorphisms with gastric cancer risk in Asian populations[J]. World J Gastroenterol, 2015, 21(18):5707-5718.
[18]
Maalmi H, Beraies A, Charad R, et al. IL-17A and IL-17F genes variants and susceptibility to childhood asthma in Tunisia[J]. J Asthma, 2014, 51(4):348-354.
Vargas-Alarcón G, Angeles-Martínez J, Villarreal-Molina T, et al. Interleukin-17A gene haplotypes are associated with risk of premature coronary artery disease in Mexican patients from the Genetics of Atherosclerotic Disease (GEA) study[J]. PLoS One, 2015, 10(1):e0114943.
