Abstract：Energy metabolism of mitochondria plays an important role in hypoxic ischemic encephalopathy (HIE). This study aims to investigate the changes of energy metabolism following hypoxic ischemic brain damage (HIBD) in cortical neurons of newborn rats. Methods Seven day old Wistar rats were randomly assigned into a Sham operation control group (n=6) and a Cerebral hypoxia ischemia (HI) group. The HI group was divided into 10 subgroups of 6 animals, sacrificed respectively at 0, 2, 4, 6, 8, 10, 12, 24, 48 and 72 hrs after HI. The rats in the Sham operation control group were sacrificed at 4 hrs post operatively. The concentrations of PCr, ATP, ADP and AMP in cortical neurons were measured with high performance liquid chromatography (HPLC). Results The concentrations of PCr, ATP and ATP+ADP+AMP decreased immediately after HI and reached a nadir 0-6 hrs after HI ( 51% , 71% and 50% of controls, respectively), returning almost to normal values by 8-12 hrs after HI. Twelve hrs after HI the values again decreased reaching a second nadir by 24-48 hrs post HI ( 58% , 61% , 33% of controls, respectively; P< 0.05 ). Conclusions There is a secondary decrease in high energy phosphates 24-48 hrs after cerebral HI in newborn rats. Changes of energy metabolism may serve as indicators in evaluating the efficacy of intervention for HIBD.
LI Shu-Juan,MAO Jian. Changes of energy metabol ism in the cortical neurons of newborn rats with cerebral hypoxia2ischemia[J]. CJCP, 2004, 6(2): 101-104.
