ACE基因插入或缺失多态性与NICU新生儿左心室质量的关系

韩彤妍; 王新利; 崔蕴璞; 叶鸿瑁; 李在玲; 童笑梅; 朴梅花; 李松

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中国当代儿科杂志 ›› 2010, Vol. 12 ›› Issue (10) : 767-770.

论著·临床研究

ACE基因插入或缺失多态性与NICU新生儿左心室质量的关系

韩彤妍，王新利，崔蕴璞，叶鸿瑁，李在玲，童笑梅，朴梅花，李松

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Relationship between ACE gene insertion or deletion polymorphism and left ventricular mass in newborns admitted to the neonatal intensive care unit

HAN Tong-Yan, WANG Xin-Li, CUI Yun-Pu, YE Hong-Mao, LI Zai-Ling, TONG Xiao-Mei, PIAO Mei-Hua, LI Song

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摘要

目的：探讨新生儿重症监护病房（NICU）新生儿血管紧张素转化酶（ACE）基因型插入/缺失（I/D）多态现象与生后早期左心室质量的关系。方法：72例入住NICU的新生儿纳入研究。生后3 d取末梢血提取DNA，确定ACE基因型，生后1 d进行危重病例评分，并在生后1~3 d进行超声心动检查。结果：72例新生儿中，DD基因型11例，ID基因型31例，II基因型30例， 3基因型间临床特征、出生情况、体格测量及病情轻重差异均无统计学意义。DD基因型的左心室质量指数（LVMI）（29±4 g/m2）显著低于ID＋II基因型 (35±8 g/m2)（t＝2.383，P<0.05）。结论：ACE基因型不同LVMI有显著差异，表现为DD基因型携带者的LVMI显著低于ID＋II基因型携带者，提示D等位基因与左心室肌肉生长发育有相关性。［中国当代儿科杂志，2010，12（10）：767-770］

Abstract

OBJECTIVE: To study the relationship between angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and left ventricular mass (LVM) in newborns admitted to the neonatal intensive care unit (NICU). METHODS: Seventy-two newborns admitted to the NICU were enrolled. ACE genotypes were determined by genomic DNA which was isolated from heel-prick blood. Disease status of the newborns was evaluated by the Neonatal Critical Score (draft) on postnatal day 1. LVM and LVM index (LVMI) were evaluated by echocardiography on postnatal days 1-3. RESULTS: DD genotype was identified in 11 cases, ID genotype in 31 cases, and II genotype in 30 cases. There were no significant differences in clinical characteristics, critical score and body measurements in newborns with different genotypes. The DD genotype group showed significantly lower LVMI than the group with ID＋II genotypes (29±4 g/m2 vs 35±8 g/m2; P<0.05). CONCLUSIONS: ACE gene polymorphism is associated with the LVMI in newborns admitted to the NICU. The LVMI of DD genotype carriers is significantly lower than that of ID＋II genotypes carriers, which suggests that D allele may be associated with the growth and development of left ventricular.［Chin J Contemp Pediatr, 2010, 12 (10):767-770］

导出引用

韩彤妍, 王新利, 崔蕴璞, 叶鸿瑁, 李在玲, 童笑梅, 朴梅花, 李松. ACE基因插入或缺失多态性与NICU新生儿左心室质量的关系[J]. 中国当代儿科杂志. 2010, 12(10): 767-770

HAN Tong-Yan, WANG Xin-Li, CUI Yun-Pu, YE Hong-Mao, LI Zai-Ling, TONG Xiao-Mei, PIAO Mei-Hua, LI Song. Relationship between ACE gene insertion or deletion polymorphism and left ventricular mass in newborns admitted to the neonatal intensive care unit[J]. Chinese Journal of Contemporary Pediatrics. 2010, 12(10): 767-770

中图分类号： Q754

参考文献

［1］Silva GJ, Moreira ED, Pereira AC, Mill JG, Krieger EM, Krieger JE. ACE gene dosage modulates pressure-induced cardiac hypertrophy in mice and men［J］. Physiol Genomics, 2006, 27(3): 237-244.
［2］中华医学会急诊分会儿科学组，中华医学会儿科学分会急诊学组、新生儿学组. 新生儿危重病例评分法(草案)［J］. 中华儿科杂志, 2001, 39(1): 42-43.
［3］Devereux RB, Reichek N. Echocardiographic determination of left ventricular mass in man. Anatomic validation of the method［J］. Circulation, 1977, 55(4): 613-618.
［4］Meban C. The surface area and volume of the human fetus［J］. J Anat, 1983, 137(2): 271-278.
［5］Harding D, Dhamrait S, Marlow N, Whitelaw A, Gupta S, Humphries S, et al. Angiotensin converting enzyme DD genotype is associated with worse perinatal cardiorespiratory adaptation in preterm infants［J］. J Pediatr, 2003, 143(6): 746-749.
［6］云美玲, 钟江华, 金水晶, 张勇, 周代锋, 王红霞, 等.血管紧张素转化酶基因多态性在海南黎、汉族高血压人群的对比研究［J］. 临床心血管病杂志, 2007, 23(11):843-845.
［7］Uma R, Forsyth JS, Struthers AD, Fraser CG, Godfrey V, Murphy DJ. Correlation of angiotensin converting enzyme activity and the genotypes of the I/D polymorphism in the ACE gene with preterm birth and birth weight［J］. Eur J Obstet Gynecol Reprod Biol, 2008, 141(1): 27-30.
［8］Cooper AC, Robinson G, Vinson GP, Cheung WT, Broughton Pipkin F. The localization and expression of the rennin-angiotensin system in the human placenta throughout pregnancy［J］. Placenta, 1999, 20(5-6):467-474.
［9］Nielsen AH, Schauser KH, Poulsen K. Current topic: the uteroplacental renin-angiotensin system［J］. Placenta, 2000, 21(5-6): 468-477.
［10］Hindmarsh PC, Rodeck CH, Humphries SE. Polymorphisms in the angiotensin converting enzyme gene and growth in the first year of life［J］. Ann Hum Gene, 2007, 71(2):176-184.
［11］Barker DJ. Fetal origins of coronary heart disease［J］. BMJ, 1995, 311(6998):171-174.
［12］Schwartz ML, Goldberg SJ, Wilson N, Allen HD, Marx GR. Relation of Still′s murmur, small aortic diameter and high aortic velocity［J］. Am J Cardiol, 1986, 57(15):1344-1348.
［13］Jiang B, Godfrey KM, Martyn CN, Gale CR. Birth weight and cardiac structure in children［J］. Pediatrics, 2006, 117(2): e257-e261.
［14］Martyn CN, Greenwald SE. Impaired synthesis of elastin in walls of aorta and large conduit arteries during early development as an initiating event in pathogenesis of systemic hypertension［J］. Lancet, 1997, 350(9082):953-955.
［15］Reini SA, Wood CE, Keller-Wood M. The ontogeny of genes related to ovine fetal cardiac growth［J］. Gene Expr Patterns, 2009, 9(2): 122-128.
［16］Tian XL, Pinto YM, Costerousse O, Franz WM, Lippoldt A, Hoffmann S, et al. Over-expression of angiotensin converting enzyme-1 augments cardiac hypertrophy in transgenic rats［J］. Hum Mol Genet, 2004, 13(14): 1441-1450.
［17］Montgomery HE, Clarkson P, Dollery CM, Prasad K, Losi MA, Hemingway H, et al. Association of angiotensin-converting enzyme gene I/D polymorphism with change in left ventricular mass in response to physical training［J］. Circulation, 1997, 96(3): 741-747.
［18］Osono E, Kurihara S, Hayama N, Sakurai Y, Ohwada K, Onoda N, et al. Insertion/deletion polymorphism in intron 16 of the ACE gene and left ventricular hypertrophy in patients with end-stage renal disease［J］. Am J Kidney Dis, 1998, 32(5):725-730.
［19］Nakahara K, Matsushita S, Matsuoka H, Inamatsu T, Nishinaga M, Yonawa M, et al. Insertion/deletion polymorphism in the angiotensinconverting enzyme gene affects heart weight［J］. Circulation, 2000, 101(2): 148-151.