Abstract：OBJECTIVE: To investigate whether P-selectin gene -2123 polymorphism is associated with the pathogenesis of Henoch-Schonlein purpura (HSP) in children. METHODS: Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) is used to identify the distribution of allele and genotype frequencies of P-selectin gene promoter -2123 polymorphism in 86 children with HSP (including 40 cases of purpura nephritis) and 70 healthy controls. RESULTS: Compared with the healthy controls, the frequencies of GG genotype and G allele of P-selectin promoter -2123 in children with HSP increased significantly (P<0.05). There were no significant differences in P-selectin promoter -2123 genotype and allele frequencies between the patients with and without nephritis. CONCLUSIONS: P-selectin gene promoter -2123 polymorphism appears to be associated with the pathogenesis of HSP in children.
［3］Gok F, Ugur Y, Ozen S, Dagdeviren A. Pathogenesis-related adhesion molecules in Henoch-Schonlein vasculitis［J］. Rheumatol Int, 2008, 28(4): 313-316.
［7］McEver RP, Beckstead JH, Moore KL, Marshall-Carlson L, Bainton DF. GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies［J］.J Clin lnvest, 1989, 84(l): 92-99.
［8］Dole VS, Bergmeier W, Mitchell HA, Eichenberger SC, Wagner DD. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin［J］.Blood, 2005, 106（7）: 2334-2339.
［10］Turkoz Y, Evereklioglu C, Ozkiris A, Mistik S, Borlu M, Ozerol IH, et al. Serum levels of soluble P-selectin are increased and associated with disease activity in patients with Behcet's syndrome［J］. Mediators Inflamm, 2005（4）: 237-241.
［11］Segawa C, Wada T, Takaeda M, Furuichi K, Matsuda I, Hisada Y,et al. In situ expression and soluble from of P-selectin in human glomerulone phriric［J］. Kidney Int, 1997, 52（4）:1054-1063.
［12］Zingarelli B, Salzman AL, Szabo C. Genetic disruption of poly (ADP ribose) synthetase inhibits the expression of P-selectin and intercellular adhesion molecule-1 in myocardial ischemia/reperfusion injury［J］.Circ Res, 1998, 83(l): 85-90.
