目的：分析长沙市区儿童脂联素基因+45 位点单核苷酸多态性（SNP）基因型的分布频率，探讨其与儿童肥胖症的相关性。方法：随机抽取147例肥胖儿童及 118 例健康儿童，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测脂联素基因 SNP+45 多态性位点、双抗体夹心 ELISA 测定血清脂联素。同时进行腰围（WC）、腰臀比（WHR）、体脂百分比（%BF）、收缩压（SBP）、舒张压（DBP）、空腹血糖（FPG）、血清甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹胰岛素水平（FINS）、稳态模型胰岛素抵抗指数（HOMA-IR）的测定。结果：肥胖组和正常组的SNP + 45 多态性发生频率分别为 40.5% 和 25.4%，两组间基因型分布与等位基因频率差异均有统计学意义（P<0.05）。肥胖儿童中，TT 基因型与 TG、GG 型相比，其脂联素水平高，而 %BF、HOMA-IR、TC 和 LDL-C 水平低，差异有统计学意义（P<0.05）。结论：脂联素基因SNP+45 变异可能与儿童单纯性肥胖相关，T→G 突变可增加儿童肥胖症的发生，同时使脂联素水平下降。
Abstract
OBJECTIVE: To study the distribution characteristics of adiponectin gene +45 single nucleotide polymorphisms (SNP) in Chinese children, and to determine the role of adiponectin gene +45 polymorphisms in the pathogenesis of childhood obesity. METHODS: A total of 147 Chinese obese and 118 healthy children were randomly selected and enrolled to identify adiponectin gene SNP+45 polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Plasma adiponectin levels were determined using ELISA. Waist circumference (WC), waist to hip ratio (WHR), percentage of body fat (%BF), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), serum triglycerides (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), plasma fasting insulin (FINS), and homeostasis model assessment of insulin resistance (HOMA-IR) were measured. RESULTS: The allelic frequency of adiponectin gene SNP+45 in children with obesity and healthy controls were 40.5% and 25.4%, respectively. There were significant differences in the distribution of genotypes and the allelic frequency between the two groups (P<0.05). The plasma adiponectin levels were significantly higher, in contrast, %BF, HOMA-IR, TC and LDL-C levels were significantly lower in obese children with TT genotype than those in obese children with TG or GG genotype. CONCLUSIONS: The adiponectin gene SNP+45 polymorphism may be associated with pathogenesis of obesity in children. T→G variance may be associated an increased risk of childhood obesity and result in a decreased level of adiponectin.
关键词
脂联素 /
单核苷酸多态性 /
肥胖症 /
儿童
Key words
Adiponectin /
Single nucleotide polymorphisms /
Obesity /
Child
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参考文献
［1］郑云郎.儿童肥胖的危害及应对措施［J］.中国健康教育, 2004, 20(11): 1054-1056.
［2］颜伟健,吴静,莫娟,黄超文,彭烈武,徐丽. 肥胖儿童血浆脂联素与肿瘤坏死因子-α的变化及意义［J］.中国当代儿科杂志,2009,11(1):47-50.
［3］Vionnet N, Hani EH, Dupont S,Gallina S, Francke S, Dotte S, et al. Genomewide search for type 2 diabetes-susceptibility genes in French whites: evidence for a novel susceptibility locus for early-onset diabetes on chromosome 3q27-qter and independent replication of a type 2-diabetes locus on chromosome 1q21-q24［J］. Am J Hum Genet, 2000, 67(6):1470-1480.
［4］Francke S, Manraj M, Lacquemant C, Lecoeur C, Leprêtre F, Passa P, et al. A genome-wide scan for coronary heart disease suggests in Indo-Mauritians a susceptibility locus on chromosome 16p13 and replicates linkage with the metabolic syndrome on 3q27［J］. Hum Mol Genet, 2001, 10（24）: 2751-2765.
［5］Chung HK, Chae JS, Hyun YJ, Paik JK, Kim JY, Jang Y, et al. Influence of adiponectin gene polymorphisms on adiponectin level and insulin resistance index in response to dietary intervention in overweight-obese patients with impaired fasting glucose or newly diagnosed type 2 diabetes［J］. Diabetes Care, 2009, 32(4): 552-558.
［6］中国肥胖问题工作组.中国学龄儿童青少年超重、肥胖筛查体质指数值分类标准［J］.中华流行病学杂志,2004,25(2):97-102.
［7］莫娟，吴静，孙志香，杨浩波，雷闽湘，刘玮芳.PTP1B基因多态性与儿童单纯性肥胖的关系（英文）［J］.中南大学学报（医学版）, 2010, 14(9): 1498-1504.
［8］Petrone A, Zavarella S, Caiazzo A, Leto G, Spoletini M, Potenziani S, et al. The promoter region of the adiponectin gene is a determinant in modulating insulin sensitivity in childhood obesity［J］. Obesity (Silver Spring), 2006, 14(9): 1498-1504.
［9］Bouatia-Naji N, Meyre D, Lobbens S. Séron K, Fumeron F, Balkau B, et al. ACDC/adiponectin polymorphisms are associated with severe childhood and adult obesity［J］. Diabetes, 2006, 55(2): 545-550.
［10］魏颖丽，霍正浩，赵巍. 我国西部人群脂联素基因+45位点多态性与肥胖、胰岛素抵抗及2型糖尿病的相关性［J］. 中国糖尿病杂志，2007（10）：583-585.
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